KAKEN — Research Projects | Researcher Number: 90309435

Search Results: 16 results / Researcher Number: 90309435

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1. ビタミンK変換酵素欠損マウスを用いた脂肪組織におけるビタミンKの新規機能解析

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
Research Institution	Kobe Gakuin University
Principal Investigator	中川公恵神戸学院大学, 薬学部, 教授
Project Period (FY)	2024-04-01 – 2027-03-31Granted
Keywords	ビタミンK / UBIAD1 / MK-4 / ノックアウトマウス / 脂肪組織 / menaquinone-4
Outline of Research at the Start	ヒトが摂取したビタミンKは、消化管から吸収されて体内で働くのみでなく、体内の各組織中でUbiA prenyltransferase domain containing protein 1（UBIAD1）により、ビタミンK2（メナキノン-4: MK-4）に変換され、多岐に渡る生理作用を発揮する。本研究 ...
Outline of Annual Research Achievements	ヒトが摂取したビタミンKは、消化管から吸収されて体内で働くのみでなく、体内の各組織中でUbiA prenyltransferase domain containing protein 1（UBIAD1）により、ビタミンK2（メナキノン-4: MK-4）に変換され、多岐に渡る生理作用を発揮する。申請者 ...
Current Status of Research Progress	2: Research has progressed on the whole more than it was originally planned.

Research Products of this project	Journal Article (2 results, of which Peer Reviewed: 2 results, Open Access: 1 results) Presentation (4 results)

2. Elucidation of novel physiological functions of vitamin K using time-tissue-specific vitamin K-converting enzyme-deficient mice

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
Research Institution	Kobe Gakuin University
Principal Investigator	Nakagawa Kimie 神戸学院大学, 薬学部, 教授
Project Period (FY)	2021-04-01 – 2024-03-31Completed
Keywords	ビタミンK / UBIAD1 / MK-4 / ノックアウトマウス / 骨形成 / 軟骨細胞 / 心臓 / 脂肪 / 骨 / 心血管 / 脳神経 / Menaquinone-4
Outline of Research at the Start	ヒトが主として摂取するビタミンKは植物由来のビタミンK1（フィロキノン, PK）を生体内でMK-4に変換する酵素であるUbiA prenyltransferase domain containing 1（UBIAD1）の全身欠損マウスは、個体発生に必須の機能を担う。UBIAD1は全身のあらゆる組織に ...
Outline of Final Research Achievements	The role of UbiA prenyltransferase domain containing protein 1 (UBIAD1), the enzyme that converts vitamin K to menaquinone-4 (MK-4) in the body, in os ...

Research Products of this project	Journal Article (3 results, of which Peer Reviewed: 2 results, Open Access: 2 results) Presentation (35 results, of which Int'l Joint Research: 2 results, Invited: 6 results)

3. Research on new vitamin K functions aimed at application to prevention and treatment of cardiovascular diseases

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Review Section	Basic Section 47030:Pharmaceutical hygiene and biochemistry-related
Research Institution	Kobe Gakuin University (2020) Kobe Pharmaceutical University (2018-2019)
Principal Investigator	Nakagawa Kimie 神戸学院大学, 薬学部, 教授
Project Period (FY)	2018-04-01 – 2021-03-31Completed
Keywords	ビタミンK / 生体内変換 / MK-4 / UBIAD1 / 心血管 / ノックアウトマウス / SM22α / コンディショナルノックアウトマウス / 心臓 / 心筋細胞 / 心血管疾患 / 血管平滑筋 / メナキノン-4
Outline of Final Research Achievements	Vitamin K is a fat-soluble vitamin that works on blood coagulation and bone formation. The vitamin K we take on a daily basis is mainly vitamin K1 con ...

Research Products of this project	Presentation (6 results, of which Invited: 2 results)

4. Construction of evaluation system for vitamin D analogs using genetically modified rats with genome editing

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Applied biochemistry
Research Institution	Toyama Prefectural University
Principal Investigator	SAKAKI TOSHIYUKI 富山県立大学, 工学部, 教授
Project Period (FY)	2016-04-01 – 2019-03-31Completed
Keywords	ビタミンD / ゲノム編集 / ビタミンD受容体 / シトクロムP450 / 遺伝子改変 / ビタミンD代謝酵素 / 作用メカニズム / 疾患モデル動物 / 遺伝子 / ゲノム / 酵素 / シグナル伝達 / 骨粗鬆症 / 遺伝子改変ラット / 癌
Outline of Final Research Achievements	The gene-deficient rats of CYP27B1, CYP24A1, or vitamin D receptor were successfully produced by the genome-editing method using the CRISPR/Cas9 syste ...

Research Products of this project	Journal Article (15 results, of which Int'l Joint Research: 4 results, Peer Reviewed: 13 results, Open Access: 3 results) Presentation (49 results, of which Int'l Joint Research: 3 results, Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

5. Generation and characterization of brain- specific UBIAD1-deficient mice using a nestin

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	Nakagawa Kimie 神戸薬科大学, 薬学部, 准教授
Project Period (FY)	2014-04-01 – 2017-03-31Completed
Keywords	ビタミンK / UBIAD1 / メナキノン-4 / Nestin-cre / 脳神経 / コンディショナルノックアウトマウス / Nestin / ノックアウトマウス / vitamin K / MK-4 / brain / Menaquinone-4 / コンディショナルノックアウト
Outline of Final Research Achievements	In this study, we created Nestin-cre Ubiad1-CKO mice which were Ubiad1 deficient from the time of neural stem cell. Nestin-cre Ubiad1-CKO was born nor ...

Research Products of this project	Journal Article (2 results, of which Peer Reviewed: 2 results, Open Access: 1 results, Acknowledgement Compliant: 2 results) Presentation (8 results, of which Int'l Joint Research: 1 results, Invited: 2 results) Book (1 results)

6. The vitamin K biosynthetic mechanism in brain and its relevance to neurodegenerative disease

Research Project

Research Category	Grant-in-Aid for Young Scientists (B)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	NAKAGAWA Kimie 神戸薬科大学, 薬学部, 准教授
Project Period (FY)	2011 – 2012Completed
Keywords	分子栄養学 / ビタミンK / メナキノン-4 / メナキノン-4生合成酵素 / アルツハイマー / 脳神経
Research Abstract	Vitamin K is a cofactor for γ-glutamyl carboxylase (GGCX), which catalyses the post-translational modifications of several vitamin K-dependent protein ...

Research Products of this project	Journal Article (3 results, of which Peer Reviewed: 1 results) Presentation (24 results) Book (2 results) Remarks (1 results)

7. Generation and characterization of genetically-altered mice lacking a novel vitamin K2 synthesis enzyme Ubiad1

Research Project

Research Category	Grant-in-Aid for Scientific Research (B)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	OKANO TOSHIO 神戸薬科大学, 薬学部, 教授
Project Period (FY)	2011-04-01 – 2014-03-31Completed
Keywords	遺伝子改変動物 / ビタミンK2 / 合成酵素 / UBIAD1 / 全身性ノック・アウト / コンデショナル・ノックアウト / 神経 / 骨 / ビタミンＫ2 / ＵＢＩＡＤ1 / 全身性ノックアウト / コンディショナル・ノックアウト / ヒト / 遺伝子欠損動物 / CAG-Creヘテロマウス / CAG-Flpヘテロマウス
Research Abstract	It was demonstrated that tissue vitamin K2 originates from oral vitamin K1 by a mechanism that involves the release of vitamin K3 from vitamin K1 in t ...

Research Products of this project	Journal Article (39 results, of which Peer Reviewed: 24 results) Presentation (98 results, of which Invited: 11 results) Book (13 results) Remarks (2 results)

8. 25-ヒドロキシビタミンD3のホルモン作用の解析

Research Project

Research Category	Grant-in-Aid for Challenging Exploratory Research
Research Field	General physiology
Research Institution	Toyama Prefectural University
Principal Investigator	榊利之富山県立大学, 工学部, 教授
Project Period (FY)	2010Completed
Keywords	ビタミンD / シトクロムP450 / 代謝 / 細胞増殖抑制 / ノックアウトマウス / DNAマイクロアレイ
Research Abstract	従来、25-ヒドロキシビタミンD_3(25D3)は1α位水酸化酵素CYP27B1により1α,25-ジヒドロキシビタミンD_3(1,25D3)に変換され生理作用を示すと考えられてきたが、組織によっては25D3が直接作用している可能性がある。ヒト前立腺由来培養細胞(PZ-HPV-7)を種々の濃度の1,2 ...

Research Products of this project	Journal Article (1 results, of which Peer Reviewed: 1 results) Presentation (5 results) Book (1 results)

9. Analysis of molecular nutritional function of vitamin D and vitamin K using targeted gene knockout cells and mice.

Research Project

Research Category	Grant-in-Aid for Young Scientists (B)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	NAKAGAWA Kimie Kobe Pharmaceutical University
Project Period (FY)	2008 – 2009Completed
Keywords	ビタミン / ビタミンD受容体 / CYP27B1 / 1α,25-D3 / ノックアウトマウス / ビタミンK / MK-4 / SXR / ビタミンD / 1α, 25-D3
Research Abstract	Vitamin D plays an important role in regulation of calciumhomeostasis and bone metabolism. Among vitamin D metabolites, 25-hydroxyvitamin D 25-OH-D) c ...

Research Products of this project	Journal Article (12 results, of which Peer Reviewed: 8 results) Presentation (15 results)

10. Construction of an arteriosclerosis assessment system using genetically modified cells and animals, and drug development

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	OKANO Toshio Kobe Pharmaceutical University
Project Period (FY)	2008 – 2010Completed
Keywords	分子生物学 / 遺伝子改変細胞 / 遺伝子改変動物 / 動脈硬化モデル / ビタミンD / ビタミンK / 生合成酵素 / 活性評価 / 活性型ビタミンD / 受容体 / 活性化酵素
Research Abstract	To elucidate the role of vitamin D in the prevention and treatment of arteriosclerosis, we have generated vitamin D receptor(VDR) gene knockout mice o ...

Research Products of this project	Journal Article (44 results, of which Peer Reviewed: 38 results) Presentation (55 results) Book (13 results) Remarks (1 results)

11. Construction of gene targeting animal models for evaluating preventive and therapeutic anti-cancer effectiveness of active vitamin D analogues

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	OKANO Toshio Kobe Pharmaceutical University, DEPARTMENT OF HYGIENIC SCIENCES, PROFESSOR
Project Period (FY)	2006 – 2007Completed
Keywords	VITAMIN D RECEPTOR / VITAMIND 1α HYDROXYLASE / GENE-TARGETING ANIMALS / METASTASIS OF CANCER CELLS / CANCER CELL GROWTH / GENE-TARGETING CELLS / CANCER CELL-INDUCED ANGIOGENESIS / NOVEL ACTIVE VITAMIN D ANALOGUES
Research Abstract	We have generated a line of animal model (1αOHase-/-) lacking the gene encoding vitamin D la-hydroxylase that is a key enzyme for activation of vitami ...

Research Products of this project	Journal Article (19 results, of which Peer Reviewed: 10 results) Presentation (15 results) Book (10 results)

12. Elucidation of the mechanism of cancer cell metastasis and permeation using active vitamin D as a molecular base and development of therapeutic agents

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	KOBE PHARMACEUTICAL UNIVERSITY
Principal Investigator	OKANO Toshio KOBE PHARMACEUTICAL UNIVERSITY, DEPARTMENT OF HYGIENIC SCIENCES, PROFESSOR
Project Period (FY)	2003 – 2005Completed
Keywords	ACTIVE VITAMIN D ANALOGS / VITAMIN D RECEPTOR / CHEMICAL STRUCTURE-ACTIVITY RELATIONSHIP / VITAMIN D METABOLISM / APOPTOSIS / CELL GROWTH AND DIFFERENTIATION / TUMOR INVASION AND METASTASIS / ANGIOGENESIS
Research Abstract	In the present study, we demonstrated from the in vitro and in vivo studies that 1α,25-dihydroxyvitamin D_3 (1α,25-D_3) and its derivatives are effect ...

Research Products of this project	Journal Article (40 results) Book (7 results) Publications (9 results)

13. がん治療薬ならびに転移抑制薬となる新規ビタミンD誘導体の開発

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas (C)
Review Section	Biological Sciences
Research Institution	Kobe Pharmaceutical University
Principal Investigator	中川公恵神戸薬科大学, 薬学部, 助手
Project Period (FY)	2001Completed
Keywords	活性型ビタミンD誘導体 / がん細胞 / 構造と活性 / 細胞の増殖・分化 / 遺伝子転写調節 / アポトーシス / 細胞周期 / ビタミンD受容体
Research Abstract	がん治療薬として臨床応用可能な活性型ビタミンD誘導体を創製することを目標に、新規活性型ビタミンD誘導体について細胞レベルで活性スクリーニング、作用メカニズムの解明を行った。まず、現在所有する数種の活性型ビタミンD誘導体および新規に開発した誘導体を用いて活性スクリーニングを行った結果、活性型ビタミンD ...

Research Products of this project	Publications (7 results)

14. DEVELOPMENT OF VITAMIN D ANALOGS POSSESSING STRUCTURAL SELECTIVE REGULATORY ACTIVITY TOWARDS CANCER CELL DIFFERENTIATION AND APOPTOSIS

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	KOBE PHARMACEUTICAL UNIVERSITY
Principal Investigator	OKANO Toshio KOBE PHARMACEUTICAL UNIVERSITY, DEPARTMENT OF HYGIENIC SCIENCES, PROFESSOR
Project Period (FY)	2000 – 2002Completed
Keywords	ACTIVE VITAMIN D ANALOGS / CANCER CELLS / STRUCTURE AND ACTIVITY / CELL DIFFERENTIATION - PROLIFERATION / GENE REGULATION / APOPTOSIS / CELL CYCLE / VITAMIN D RECEPTOR
Research Abstract	It has been well documented in vitro and in vivo that 1a, 25-dihydroxyvitamin D3 (1a, 25(OH)2D3), an active form of vitamin D3, exhibits a variety of ...

Research Products of this project	Publications (43 results)

15. がん治療を目的とした新規ビタミンD_3誘導体の開発研究

Research Project

Research Category	Grant-in-Aid for Scientific Research on Priority Areas (C)
Review Section	Biological Sciences
Research Institution	Kobe Pharmaceutical University
Principal Investigator	津川尚子神戸薬科大学, 薬学部, 講師
Project Period (FY)	2000Completed
Keywords	活性型ビタミンD誘導体 / がん治療 / 構造と活性 / 細胞の分化・増殖 / 遺伝子転写調節 / アポトーシス / p21 / ビタミンD受容体
Research Abstract	がん治療への活性型ビタミンDの応用を目的として、1)分化誘導能能及びアポトーシス誘導能が高く、カルシウム作用の低い誘導体を得ること、2)誘導体による細胞分化、アポトーシス誘導の作用機序を解明すること、3)多種の誘導体のスクリーニングから、構造と活性の相関性を見いだし、今後の誘導体設計に役立てること、 ...

Research Products of this project	Publications (9 results)

16. Structure-Activity Study of Vitamin D Analogues

Research Project

Research Category	Grant-in-Aid for Scientific Research (C)
Research Field	Biological pharmacy
Research Institution	Kobe Pharmaceutical University
Principal Investigator	OKANO Toshio Kobe Pharmaceutical University, Hygienic Sciences, Professor
Project Period (FY)	1997 – 1999Completed
Keywords	Active vitamin D analogues / Synthesis / Structure-Function / Cell differentiation and proliferation / Regulation of gene transcription / Apoptosis / Intracellular calcium / Osteoporosis
Research Abstract	To clarify the structure-function relationship of vitamin D analogues, we synthesized novel vitamin D analogues and evaluated their biological activit ...

Research Products of this project	Publications (56 results)