Studies on odorant and pheromone receptor as a chemosensor for the external environmen

• Project Area: Molecular interaction and modal shift of cellular sensors
• Project/Area Number: 18077001
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: TOUHARA Kazushige The University of Tokyo, 大学院・農学生命科学研究科, 教授 (00280925)
• Project Period (FY): 2006 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥78,100,000 (Direct Cost: ¥78,100,000); Fiscal Year 2010: ¥14,500,000 (Direct Cost: ¥14,500,000); Fiscal Year 2009: ¥17,300,000 (Direct Cost: ¥17,300,000); Fiscal Year 2008: ¥17,600,000 (Direct Cost: ¥17,600,000); Fiscal Year 2007: ¥13,900,000 (Direct Cost: ¥13,900,000); Fiscal Year 2006: ¥14,800,000 (Direct Cost: ¥14,800,000)
• Keywords: 匂い / フェロモン / 嗅覚 / センサー / 受容体 / チャネル / 昆虫 / マウス / カイコ / ショウジョウバエ / 嗅覚受容体 / 誘引

Research Abstract

Animals are attracted by general odorants derived from food or by pheromones released from the opposite sex. The chemical information is detected by seven-transmembrane chemosensory receptors expressed by peripheral olfactory sensory neurons. In this project, we revealed the molecular mechanisms underlying chemosensory reception and the neural circuitry activated by the chemosensory receptors for a pheromone or a biologically important odorant that is crucial for survival or mating in various animal species.

Research Products

• [Journal Article] Enzymatic conversion of odorants in nasal mucus affects olfactory glomerular activation patterns and odor perception (2010)
  • Author(s): Nagashima, A., Touhara, K.
  • Journal Title: J.Neurosci. 30 Pages: 16391-16398
  • Peer Reviewed
• [Journal Article] Adiponectin and AdipoR1 regulate PGC-1alpha and mitochondria by Ca(2+) and AMPK/SIRT1 (2010)
  • Author(s): Iwabu M, Yamauchi T, Okada-Iwabu M, Sato K, Nakagawa T, Funata M, Yamaguchi M, Namiki S, Nakayama R, Tabata M, Ogata H, Kubota N, Takamoto I, Hayashi YK, Yamauchi N, Waki H, Fukayama M, Nishino I, Tokuyama K, Ueki K, Oike Y, Ishii S, Hirose K, Shimizu T, Touhara K, Kadowaki T.
  • Journal Title: Nature 464 Pages: 1313-1319
  • Peer Reviewed
• [Journal Article] The male mouse pheromone ESP1 enhances female sexual receptive behavior through a specific vomeronasal receptor (2010)
  • Author(s): Haga, S., Hattori, T., Sato, T., Sato, K., Matsuda, S., Kobayakawa, R., Sakano, H., Yoshihara, Y., Kikusui, T., Touhara, K.
  • Journal Title: Nature 466 Pages: 118-122
  • Peer Reviewed
• [Journal Article] Adiponectin and AdipoR1 regulate PGC-1alpha and mitochondria by Ca(2+) and AMPK/SIRT1 (2010)
  • Author(s): Iwabu M, Yamauchi T, Okada-Iwabu M, Sato K, Nakagawa T, Funata M, Yamaguchi M, Namiki S, Nakayama R, Tabata M, Ogata H, Kubota N, Takamoto I, Hayashi YK, Yamauchi N, Waki H, Fukayama M, Nishino I, Tokuyama K, Ueki K, Oike Y, Ishii S, Hirose K, Shimizu T, Touhara K, Kadowaki T.
  • Journal Title: Nature Volume: 464 Pages: 1313-119
  • Peer Reviewed
• [Journal Article] The male mouse pheromone ESP1 enhances female sexual receptive behaviour through a specific vomeronasal receptor (2010)
  • Author(s): Haga, S., Hattori, T., Sato, T., Sato, K., Matsuda, S., Kobayakawa, R., Sakano, H., Yoshihara, Y., Kikusui, T., Touhara, K.
  • Journal Title: Nature Volume: 466 Pages: 118-122
  • Peer Reviewed
• [Journal Article] Enzymatic conversion of odorants in nasal mucus affects olfactory glomerular activation patterns and odor perception (2010)
  • Author(s): Nagashima, A., Touhara, K.
  • Journal Title: J.Neurosci. Volume: 30 Pages: 16391-16398
  • Peer Reviewed
• [Journal Article] Chemical Identity of a Rotting Animal-Like Odor Emitted from the Inflorescence of the Titan Arum (Amorphophallus titanum) (2010)
  • Author(s): Shirasu, M., Fujioka, K., Kakishima, S., Nagai, S., Tomizawa, Y., Tsukaya, H., Murata J., Manome, Y., Touhara, K.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 2550-2554
  • NAID: 10027728626
  • Peer Reviewed
• [Journal Article] Sensing odorants and pheromones with chemosensory receptors (2009)
  • Author(s): Touhara, K., Vosshall, L.B.
  • Journal Title: Annu.Rev.Physiol. 71 Pages: 307-332
• [Journal Article] Two chemoreceptors mediate the developmental effects of dauer pheromone in C.elegans (2009)
  • Author(s): Kim, K., Sato, K., Shibuya, M., Zeiger, D.M., Butcher, R.A., Ragains, J.R., Clardy, J., Touhara, K., Sengupta, P.
  • Journal Title: Science 326 Pages: 994-998
  • Peer Reviewed
• [Journal Article] Nasal airflow rate affects the sensitivity and pattern of glomerular odorant responses in the mouse olfactory bulb (2009)
  • Author(s): Oka, Y., Takai, Y., Touhara, K.
  • Journal Title: J.Neurosci. 29 Pages: 12070-12078
  • Peer Reviewed
• [Journal Article] Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile (2009)
  • Author(s): Tanaka, K., Uda, Y., Ono, Y., Nakagawa, T., Suwa, M., Yamaoka, R., Touhara, K.
  • Journal Title: Current Biology 19 Pages: 881-890
  • Peer Reviewed
• [Journal Article] Dimethyl trisulfide as a characteristic odor associated with fungating cancer wounds (2009)
  • Author(s): Shirasu, M., Nagai, S., Hayashi, R., Ochiai, A., Touhara, K.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 2117-2120
  • NAID: 10027546152
  • Peer Reviewed
• [Journal Article] Mammalian olfactory receptors : pharmacology, G protein-coupling and desensitization (2009)
  • Author(s): Kato, A., Touhara, K.
  • Journal Title: Cell.Mol.Life Sci. 66 Pages: 3743-3753
  • Peer Reviewed
• [Journal Article] Myr-Ric8A enhances Gaα15-mediated Ca2+ response of vertebrate olfactory receptors (2009)
  • Author(s): Yoshikawa, K., and Touhara, K.
  • Journal Title: Chem. Senses 34 Pages: 15-23
  • Peer Reviewed
• [Journal Article] Myr-Ric8A enhances Gal 5-mediated Ca^<2+> response of vertebrate olfactcry receptors (2009)
  • Author(s): Yoshikawa, K., and Touhara, K.
  • Journal Title: Chem. Senses 34 Pages: 15-23
  • Peer Reviewed
• [Journal Article] Insect olfactory receptors are heteromeric ligand-gated channels (2008)
  • Author(s): Sato, K., Pellegrino, M., Nakagawa, T., Nakagawa, T., Vosshall, L.B., Touhara, K.
  • Journal Title: Nature 452 Pages: 1002-1006
  • Peer Reviewed
• [Journal Article] Amino acids involved in conformational dynamics and G-protein coupling of an odorant receptor : targeting gain-of-function mutation (2008)
  • Author(s): Kato, A., Katada, S., Touhara, K.
  • Journal Title: J.Neurochem. 107 Pages: 1261-1270
  • Peer Reviewed
• [Journal Article] Amino acids involved in conformational dynamics and G-protein coupling of an odorant receptor: targeting gain-of-function mutation (2008)
  • Author(s): Kato, A., Katada, S., and Touhara, K.
  • Journal Title: J. Neurochem. 107 Pages: 1261-1270
  • Peer Reviewed
• [Journal Article] Insect olfactory receptors are heteromeric ligand-gated channels (2008)
  • Author(s): Sato, K., Pellegrino, M., Nakagawa, T., Nakagawa, T., Vosshall, L.B., and Touhara, K.
  • Journal Title: Nature 452 Pages: 1002-1006
  • Peer Reviewed
• [Journal Article] Amino acids involved in conformational dynamics and G-protein coupling of an odorant receptor : targeting gain-of-function mutation (2008)
  • Author(s): Kato, A., Katada, S., and Touhara, K.
  • Journal Title: J. Neurochem 107 Pages: 1261-1270
  • Peer Reviewed
• [Journal Article] Insect olfactory receptors are heteromeric ligand-gated channels (2008)
  • Author(s): Sato, K., Pellegrino, M., Nakagawa, T., Nakagawa, T., Vosshall, L.B and Touhara, K.
  • Journal Title: Nature 452 Pages: 1002-1006
  • Peer Reviewed
• [Journal Article] The mouse ESP family : sex and strain differences, and implications in the vomeronasal sensory system (2007)
  • Author(s): Kimoto, H., Sato, K., Nodari, F., Haga, S., Holy, T., Touhara, K.
  • Journal Title: Current Biology 17 Pages: 1879-1884
  • Peer Reviewed
• [Journal Article] The mouse ESP family: sex and strain differences, and implications in the vomeronasal sensory system (2007)
  • Author(s): Kimoto, H., Sato, K., Nodari, F., Haga, S., Holy, T., and Touhara, K.
  • Journal Title: Current Biology 17 Pages: 1879-1884
  • Peer Reviewed
• [Journal Article] 個体生存戦略としてのセンサー機能のモーダルシフト (2007)
  • Author(s): 東原和成
  • Journal Title: 細胞工学 26 Pages: 870-871
• [Journal Article] Deorphanizing vertebrate olfactory receptors : recent advances in odorant response assays (2007)
  • Author(s): Touhara K.
  • Journal Title: Neurochemistry Internatl. 51 Pages: 132-139
  • Peer Reviewed
• [Journal Article] 匂いの分子生物学 (2007)
  • Author(s): 岡勇輝、東原和成
  • Journal Title: Annual Review神経2007 Pages: 8-14
• [Journal Article] 細胞膜フェロモンセンサー:化学情報伝達の分子機構 (2007)
  • Author(s): 佐藤幸治、東原和成
  • Journal Title: Bionics 4 Pages: 30-35
• [Journal Article] Odorant receptor map in the mouse olfactory bulb : in vivo sensitivity and specificity of receptor-defined glomeruli (2006)
  • Author(s): Oka, Y., Katada, S., Omura, M., Suwa, M., Yoshihara, Y., Touhara, K.
  • Journal Title: Neuron 52 Pages: 857-869
  • Peer Reviewed
• [Journal Article] Odorant receptor map in the mouse olfactory bulb: in vivo sensitivity and specificity of receptor-defined glomeruli (2006)
  • Author(s): Oka, Y., Katada, S., Omura, M., Suwa, M., Yoshihara, Y. and Touhara, K.
  • Journal Title: Neuron 52 Pages: 857-869
  • Peer Reviewed
• [Journal Article] Odorant receptor map in the mouse olfactory bulb: in vivo sensitivity and specificity of receptor-defined glomeruli (2006)
  • Author(s): Oka, Y., Katada, S., Omura, M., Suwa, M., Yoshihara, Y., and Touhara, K.
  • Journal Title: Neuron 52 Pages: 856-869
• [Presentation] ショクダイオオコンニャクが放つ悪臭の成分分析 (2011)
  • Author(s): 白須未香、藤岡宏樹、柿嶋聡、長井俊治、冨澤康子、塚谷裕一、邑田仁、馬目佳信、東原和成
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大
  • Year and Date: 2011-03-26
• [Presentation] マウス嗅粘液中で起きる匂い物質変換反応は匂い知覚に影響を与える (2010)
  • Author(s): 永嶌鮎美、東原和成
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-07
• [Presentation] Enzymatic conversion of odorants in nasal mucus affects olfactory glomerular activation patterns and odor perception (2010)
  • Author(s): Nagashima A., Touhara K.
  • Organizer: The 8th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 九州大学
  • Year and Date: 2010-11-06
• [Presentation] Chemosensory receptor and behavior. (2010)
  • Author(s): Touhara, K.
  • Organizer: International Titisee Conferences
  • Place of Presentation: Titisee, Germany
  • Year and Date: 2010-10-14
• [Presentation] Enzymatic conversion of odorants in nasal mucus affects olfactory glomerular activation patterns and odor perception (2010)
  • Author(s): Nagashima, A., Touhara, K.
  • Organizer: Association for Chemoreception Sciences (AChemS) 32nd Annual Meeting
  • Place of Presentation: Tampa, U.S.A.
  • Year and Date: 2010-04-23
• [Presentation] Sensing odorant and pheromone. (2010)
  • Author(s): Touhara, K.
  • Organizer: Janelia Farm Conference
  • Place of Presentation: Janelia Farm, USA
• [Presentation] Insect chemosensation via ligand-gated cation channels (2009)
  • Author(s): Sato, K., Touhara, K.
  • Organizer: European Chemoreception Research Organization Congress
  • Place of Presentation: Villasimius, Italy
  • Year and Date: 2009-09-25
• [Presentation] Putative Amino Acids Involved in Ion Channel Activities of Insect Olfactory Receptors (2009)
  • Author(s): Nakagawa, T., Sato, K., Touhara, K.
  • Organizer: European Symposium of Insect Taste and Olfaction(ESITO)
  • Place of Presentation: Sardinia, Italy
  • Year and Date: 2009-09-18
• [Presentation] Chemistry and Biology Underlying Chemotaxis Behavior in Silkworms (2009)
  • Author(s): Touhara, K.
  • Organizer: 5th Japan-Korea Young Scientist Meeting on Bioorganic and Natural Products Chemistry
  • Place of Presentation: Kanagawa, Japan
  • Year and Date: 2009-08-28
• [Presentation] Chemosensory receptors for odors and pheromones (2009)
  • Author(s): Touhara, K.
  • Organizer: International Congress of Physiological Sciences(IUPS 2009)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-31
• [Presentation] Chemosensory receptor and behavior (2009)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: Tahoe City, CA, USA
  • Year and Date: 2009-03-17
• [Presentation] The ESP peptide family and its role in chemical communication. (2009)
  • Author(s): Touhara, K.
  • Organizer: European Chemoreception Research Organization Congress.
  • Place of Presentation: Sargenia, Italy
• [Presentation] Chemosensory receptors for odors and pheromones. (2009)
  • Author(s): Touhara, K.
  • Organizer: International Congress of Physiological Sciences.
  • Place of Presentation: Kyoto
• [Presentation] Vomeronasal reception of a sex peptide pheromone ESP1 in mice : the receptor, neural circuitry, and behavior. (2009)
  • Author(s): Touhara, K.
  • Organizer: 31st Annual meeting of association for chemoreception sciences
  • Place of Presentation: Florida, USA
• [Presentation] Chemosensory receptor and behavior. (2009)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: CA, USA
• [Presentation] Emerging view of insect olfactory receptor signaling (2008)
  • Author(s): Touhara, K.
  • Organizer: International Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: SFO, CA, USA
  • Year and Date: 2008-07-25
• [Presentation] A neural circuitry activated by a sex-specific peptide pheromone in mice : sexual communication via direct contact. (2008)
  • Author(s): Touhara, K.
  • Organizer: UK-Japan FoS symposium
  • Place of Presentation: France
• [Presentation] Emerging view of insect olfactory receptor signaling. (2008)
  • Author(s): Touhara, K.
  • Organizer: International. Symposium on Olfaction and Taste (ISOT)
  • Place of Presentation: CA, USA
• [Presentation] 昆虫の7回膜貫通型嗅覚受容体複合体は匂い活性型イオンチャネルである (2007)
  • Author(s): 佐藤幸治、東原成
  • Organizer: 日本分子生物学会・日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Molecular mchansims for odorant and pheromone reception (2007)
  • Author(s): Touhara, K.
  • Organizer: International symposium of National Institute of Biological Sciences in Beijing
  • Place of Presentation: Beijing, China
  • Year and Date: 2007-10-19
• [Presentation] Molecular mechanisms underlying sex pheromone reception (2007)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: Snowbard, Utah, USA
  • Year and Date: 2007-01-23
• [Presentation] Molecular mechanisms underlying sex pheromone reception. (2007)
  • Author(s): Touhara, K.
  • Organizer: Keystone Symposium on Chemical Senses
  • Place of Presentation: Utah, USA
• [Presentation] Pharmacology of mouse olfactory receptors (2006)
  • Author(s): Touhara, K.
  • Organizer: 28th Annual meeting for Association for Chemoreception Society
  • Place of Presentation: Sarasota, Florida, USA
  • Year and Date: 2006-04-26
• [Book] Encyclopedia of Neuroscience Vol.7. (2009)
  • Author(s): Touhara, K.
  • Publisher: Oxford, Academic Press
• [Book] Handbook of Neurochemistry and Molecular Neurobiology Vol.1 Signaling in Neural Development and Function (2009)
  • Author(s): Touhara, K.
  • Publisher: Springer
• [Book] Sensing odorants and pheromones with chemosensory receptors, Annu. Rev. Physiol. 71 (2009)
  • Author(s): Touhara, K. and Vosshall, L.B.
• [Book] The Senses : a comprehensive reference, Vol.4, Olfaction & Taste (2008)
  • Author(s): Touhara, K.
  • Publisher: San Diego, Academic Press
• [Book] Structure, Expression, and Function of Olfactory receptors, In: The Senses: a comprehensive reference, Vol. 4, Olfaction & Taste 4 (2008)
  • Author(s): Touhara, K.
• [Book] 香りを感知する嗅覚のメカニズム (2007)
  • Author(s): 東原和成
  • Total Pages: 66
  • Publisher: 八十八出版
• [Book] Molecular Biology of Peptide Pheromone Production and Reception in Mice, Advances in Genetics 59 (2007)
  • Author(s): Touhara, K.
• [Book] 香りを感知する嗅覚のメカニズム (2007)
  • Author(s): 東原和成
  • Publisher: 八十一出版
• [Book] G protein coupled receptors : structure, function, and ligand screening (2006)
  • Author(s): Touhara, K., Katada, S., Nakagawa, T., Oka, Y.
  • Publisher: CRC
• [Remarks] ホームページ等
  • URL: http://park.itc.u-tokyo.ac.jp/biological-chemistry/
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/biological-chemistry/
• [Remarks] 研究グループホームページアドレス
  • URL: http://park.itc.u-tokyo.ac.jp/molecular-recognition/touhara/kyukaku.html
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/molecular-recognition/touhara/kyukaku.html
• [Remarks]
  • URL: http://park.itc.u-tokyo.ac.jp/molecular-recognition/touhara/kyukaku.html
• [Patent(Industrial Property Rights)] マウスフェロモン (2009)
  • Inventor(s): 東原和成, 菊水健史, 寺沢宏明
  • Industrial Property Rights Holder: 東京大学、麻布大学、熊本大学
  • Filing Date: 2009-09-14