Elucidation of structural basis of dynamically-driven protein allostery by time-resolved resonance Raman spectroscopy

• Project Area: Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process
• Project/Area Number: 19056013
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Osaka University
• Principal Investigator: MIZUTANI Yasuhisa 大阪大学, 大学院・理学研究科, 教授 (60270469)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Haruto 大阪大学, 大学院・理学研究科, 講師 (40551338) ; MIZUNO Misao 大阪大学, 大学院・理学研究科, 助教 (10464257)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥69,200,000 (Direct Cost: ¥69,200,000); Fiscal Year 2011: ¥5,600,000 (Direct Cost: ¥5,600,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥9,100,000); Fiscal Year 2009: ¥8,000,000 (Direct Cost: ¥8,000,000); Fiscal Year 2008: ¥29,700,000 (Direct Cost: ¥29,700,000); Fiscal Year 2007: ¥16,800,000 (Direct Cost: ¥16,800,000)
• Keywords: ラマン分光法 / 生物物理学 / 時間分解分光学 / 共鳴ラマン分光法 / 時間分解分光法 / アロステリックタンパク質

Research Abstract

Protein dynamics highly relevant to biological function were studied with time-resolved resonance Raman spectroscopy. Our effort on developments on the measurement system enabled us to perform site-specific observation on structural changes of proteins in wide time range from picoseconds to milliseconds. We elucidated functionally-important dynamics of various allosteric proteins such as hemoglobins, heme-based gas sensors, and photoactive proteins with the high performance of the system.

Research Products

• [Journal Article] Differences between Protein Dynamics of Hemoglobin upon Dissociation of Oxygen and Carbon Monoxide (2012)
  • Author(s): Yuka Murakawa, Masako Nagai and Yasuhisa Mizutani
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Issue: 3 Pages: 1434-1437
  • DOI: 10.1021/ja209659w
  • Peer Reviewed
• [Journal Article] Protein Dynamics of Isolated Chains of Recombinant Human Hemoglobin Elucidated by Time-resolved Resonance Raman Spectroscopy (2012)
  • Author(s): Kenta Yamada, Haruto Ishikawa, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 116 Issue: 6 Pages: 1992-1998
  • DOI: 10.1021/jp2114303
  • Peer Reviewed
• [Journal Article] Ultrafast Protein Dynamics of Hemoglobin as Studied by Picosecond Time-resolved Resonance Raman Spectroscopy (2012)
  • Author(s): Yasuhisa Mizutani
  • Journal Title: Chem.Phys. Volume: 396 Pages: 45-52
  • DOI: 10.1016/j.chemphys.2011.05.012
  • Peer Reviewed
• [Journal Article] Direct observation of the structural change of Tyr174 in the primary reaction of sensory rhodopsin II (2011)
  • Author(s): Misao Mizuno, Yuki Sudo, Michio Homma and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 50 Issue: 15 Pages: 3170-3180
  • DOI: 10.1021/bi101817y
  • Peer Reviewed
• [Journal Article] Changes in the Hydrogen-Bond Network around the Chromophore of Photoactive Yellow Protein in the Ground and Excited States (2011)
  • Author(s): Misao Mizuno, Hironari Kamikubo, Mikio Kataoka and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 115 Issue: 29 Pages: 9306-9310
  • DOI: 10.1021/jp2029399
  • Peer Reviewed
• [Journal Article] Direct observation of vibrational energy flow in cytochrome c (2011)
  • Author(s): Naoki Fujii, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 115 Issue: 44 Pages: 13057-13064
  • DOI: 10.1021/jp207500b
  • Peer Reviewed
• [Journal Article] Protein Conformational Changes of the Oxidative-Stress Sensor, SoxR, upon Redox Change of the [2Fe-2S] Cluster Probed with Ultraviolet Resonance Raman Spectroscopy (2011)
  • Author(s): Kazuo Kobayashi, Misao Mizuno, and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 50 Issue: 44 Pages: 9468-9474
  • DOI: 10.1021/bi201526y
  • Peer Reviewed
• [Journal Article] Protein dynamics of heme-heme oxygenase-1 complex following carbon monoxide dissociation (2011)
  • Author(s): Yasuhisa Mizutani、Masato Yamaoka
  • Journal Title: J.Raman Spectrosc. Volume: 42 Issue: 5 Pages: 910-916
  • DOI: 10.1002/jrs.2797
  • Peer Reviewed
• [Journal Article] タンパク質ダイナミクスの実時間観測 (2011)
  • Author(s): 水谷泰久
  • Journal Title: パリティ Volume: 26 Pages: 52-56
  • Peer Reviewed
• [Journal Article] ピコ秒時間分解紫外共鳴ラマン分光法でみたタンパク質分子の光反応応答 (2011)
  • Author(s): 水谷泰久
  • Journal Title: 生物物理 Volume: 51 Pages: 10-13
  • NAID: 10027414703
  • Peer Reviewed
• [Journal Article] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin: Primary Protein Response to the Photoisomerization of Retinal (2009)
  • Author(s): Misao Mizuno, Mikihiro Shibata, Junya Yamada, Hideki Kandori, and Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B Volume: 113 Issue: 35 Pages: 12121-12128
  • DOI: 10.1021/jp904388w
  • Peer Reviewed
• [Journal Article] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin: Primary Protein Response to the Photoisomerization of Retinal (2009)
  • Author(s): Misao Mizuno, Mikihiro Shibata, Junya Yamada, Hideki Kandori, Yasuhisa Mizutani
  • Journal Title: J. Phys. Chem. B 113 Pages: 12121-12128
  • Peer Reviewed
• [Journal Article] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin : Primary Protein Response to the Photoisomerization of Retinal (2009)
  • Author(s): 水野操
  • Journal Title: J.Phys.Chem.B 113 Pages: 12121-12128
  • Peer Reviewed
• [Journal Article] タンパク質ダイナミクス観測のための時間分解共鳴ラマン分光計開発 (2009)
  • Author(s): 水野操
  • Journal Title: レーザー研究 37 Pages: 748-753
  • NAID: 10025517359
  • Peer Reviewed
• [Journal Article] Photoinduced Electron Transfer in Glucose Oxidase: a Picosecond Time-resolved Ultraviolet Resonance Raman Study (2008)
  • Author(s): Akiko Fujiwara and Yasuhisa Mizutani
  • Journal Title: J. Raman Spectrosc. Volume: 39 Issue: 11 Pages: 1600-1605
  • DOI: 10.1002/jrs.2077
  • Peer Reviewed
• [Journal Article] Photoinduced Electron Transfer in Glucose Oxidase: a Picosecond Time-resolved Ultraviolet Resonance Raman Study (2008)
  • Author(s): Akiko Fujiwara, Yasuhisa Mizutani
  • Journal Title: J. Raman Spectrosc 39 Pages: 1600-1605
  • Peer Reviewed
• [Journal Article] Photoinduced Electron Transfer in Glucose Oxidase : a Picosecond Time-resolved Ultraviolet Resonance Raman Study (2008)
  • Author(s): Akiko Fujiwara
  • Journal Title: J. Raman Spectrosc 39 Pages: 1600-1605
  • Peer Reviewed
• [Journal Article] 共鳴効果を利用する生体分子の部位特異的ダイナミクス観測 (2008)
  • Author(s): 永野操
  • Journal Title: 分光研究 57 Pages: 179-194
  • Peer Reviewed
• [Journal Article] Resonance Raman Observation of the Structural Dynamics of FixL on Ligand Recognition and Signaling (2007)
  • Author(s): Yusuke Hiruma, Akihiro Kikuchi, Atsunari Tanaka, Yoshitsugu Shiro, and Yasuhisa Mizutani
  • Journal Title: Biochemistry Volume: 46 Issue: 20 Pages: 6086-6096
  • DOI: 10.1021/bi062083n
  • Peer Reviewed
• [Journal Article] Primary protein response after ligand photodissociation in carbonmonoxy myoglobin (2007)
  • Author(s): Akira Sato, Ying Gao, Teizo Kitagawa, and Yasuhisa Mizutani
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 104 Issue: 23 Pages: 9627-9632
  • DOI: 10.1073/pnas.0611560104
  • Peer Reviewed
• [Journal Article] Resonance Raman Observation of the Structural Dynamics of FixL on Ligand Recognition and Signaling (2007)
  • Author(s): Yusuke Hiruma, Akihiro Kikuchi, Atsunari Tanaka, Yoshitsugu Shiro, Yasuhisa Mizutani
  • Journal Title: Biochemistry 46 Pages: 6086-6096
  • Peer Reviewed
• [Journal Article] Primary protein response after ligand photodissociation in carbonmonoxy myoglobin (2007)
  • Author(s): Akira Sato, Ying Gao, Teizo Kitagawa, Yasuhisa Mizutani
  • Journal Title: Proc. Natl. Acad. Sci. 104 Pages: 9627-9632
  • Peer Reviewed
• [Journal Article] Resonance Raman Obesrvation of the Structural Dynamics of FixL on Ligand Tecognition and Signaling (2007)
  • Author(s): Yusuke, Hiruma
  • Journal Title: Biochemistry 46 Pages: 6086-6096
  • Peer Reviewed
• [Journal Article] Primary protein response after ligand photodissociation in carbonmonxy myoglobin (2007)
  • Author(s): Akira, Sato
  • Journal Title: Proc. Natl. Acad Sci. USA 104 Pages: 9627-9632
  • Peer Reviewed
• [Presentation] Watching ultrafast protein dynamics by time-resolved visible and ultraviolet resonance Raman spectroscopy (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Chemistry Department Seminar, National Chiao Tung University
  • Place of Presentation: National Chiao Tung University, Hsinchu, Taiwan(招待講演)
  • Year and Date: 2011-10-21
• [Presentation] ヘモグロビン単離鎖の構造ダイナミクス:α鎖、β鎖および四量体との違い (2011)
  • Author(s): 水谷泰久、山田健太
  • Organizer: 第5回分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-22
• [Presentation] アカガイ二量体ヘモグロビンの構造ダイナミクス:四量体ヘモグロビンとの比較 (2011)
  • Author(s): 水谷泰久、三城佑樹
  • Organizer: 第5回分子科学討論会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-22
• [Presentation] Protein dynamics of photosensory proteins as studied by time-resolved resonance Raman spectroscopy (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県立新公会堂(奈良県)(招待講演)
  • Year and Date: 2011-07-30
• [Presentation] ハロロドプシンの光サイクル初期過程のタンパク質構造ダイナミクス:トリプトファン残基のスペクトル変化の観測 (2011)
  • Author(s): 下尾祐未、水谷泰久
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-27
• [Presentation] Watching energy flow in hemeproteins (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Place of Presentation: Telluride, Colorado, USA.(招待講演)
• [Presentation] Protein dynamics of photosensory proteins as studied by time-resolved resonance Raman spectroscopy (2011)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良県立新公会堂(招待講演)
• [Presentation] Elucidation of ultrafast protein dynamics by picosecond time-resolved visible and ultraviolet resonance Raman spectroscopy (2010)
  • Author(s): 水谷泰久
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Honolulu, Hawaii, USA(招待講演)
  • Year and Date: 2010-12-16
• [Presentation] Protein dynamics of sensory proteins as studied by time-resolved resonance Raman spectroscopy (2010)
  • Author(s): 水谷泰久
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Honolulu, Hawaii, USA(招待講演)
  • Year and Date: 2010-12-16
• [Presentation] Primary Protein Responses to Chromophore Isomerization of Photosensary Proteins (2010)
  • Author(s): 水谷泰久
  • Organizer: 22nd International Conference on Raman Spectroscopy (ICORS 2010)
  • Place of Presentation: Boston, USA(招待講演)
  • Year and Date: 2010-08-09
• [Presentation] Primary protein dynamics of rhodopains (2010)
  • Author(s): 水野操
  • Organizer: 特定領域研究「高次系分子科学」第8回ミニ公開シンポジウム(国際ミニシンポジウム)
  • Place of Presentation: 東京工業大学すずかけ台キャンパス(神奈川県)
  • Year and Date: 2010-01-15
• [Presentation] Primary protein responses to chromophore isomerization : picosecond time-resolved resonance Raman studies (2010)
  • Author(s): 水谷泰久
  • Organizer: The 6th Asian Conference on Ultrafast Phenomena
  • Place of Presentation: 台湾国立大学(台北、台湾)
  • Year and Date: 2010-01-11
• [Presentation] Elucidation of ultrafast protein dynamics by picosecond time-resolved visible and ultraviolet resonance Raman spectroscopy (2010)
  • Author(s): Yasuhia Mizutani
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA.(招待講演)
• [Presentation] Protein dynamics of sensory proteins as studied by time-resolved resonance Raman spectroscopy (2010)
  • Author(s): Yasuhia Mizutani
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA.(招待講演)
• [Presentation] Primary Protein Responses to Chromophore Isomerization of Photosensary Proteins (2010)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 22nd International Conference on Raman Spectroscopy (ICORS 2010)
  • Place of Presentation: Boston, USA.(招待講演)
• [Presentation] Primary protein responses to chromophore isomerization: picosecond time-resolved resonance Raman studies (2010)
  • Author(s): Yasuhisa Mizutani
  • Organizer: The 6th Asian Conference on Ultrafast Phenomena
  • Place of Presentation: Taipei.(招待講演)
• [Presentation] Ultrafast protein dynamics of photoactive proteins following photoisomerization : time-resolved resonance Raman studies (2009)
  • Author(s): 水谷泰久
  • Organizer: International Symposium on Protein Structures
  • Place of Presentation: 奈良先端科学技術大学院大学(奈良県)
  • Year and Date: 2009-12-17
• [Presentation] Picosecond Time-Resolved Ultraviolet Resonance Raman Spectroscopy of Bacteriorhodopsin : Protein Dynamics in Primary Photointermediates (2009)
  • Author(s): 水野操
  • Organizer: The Second Asian Spectroscopy Conference
  • Place of Presentation: ソウル国立大学(ソウル、韓国)
  • Year and Date: 2009-12-01
• [Presentation] Picosecond protein dynamics of photosensory proteins as studied by time-resolved resonance Raman spectroscopy" (2009)
  • Author(s): 水谷泰久
  • Organizer: The Second Asian Spectroscopy Conference
  • Place of Presentation: ソウル国立大学(ソウル、韓国)
  • Year and Date: 2009-12-01
• [Presentation] Ultrafast protein response to photodissociation and photoisomerization: Myoglobin and bacteriorhodopsin (2009)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 3e cycle lecture tour in Switzerland: Protein dynamics and function
  • Place of Presentation: Universitat Basel(招待講演)
  • Year and Date: 2009-10-14
• [Presentation] Ultrafast protein response to photodissociation and photoisomerization: Myoglobin and bacteriorhodopsin (2009)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 3e cycle lecture tour in Switzerland: Protein dynamics and function
  • Place of Presentation: Universitat Basel
  • Year and Date: 2009-10-14
• [Presentation] リガンド脱離後のヘムのダイナミクスに対するヘム周辺アミノ酸残基の効果:時間分解共鳴ラマン分光法による研究 (2009)
  • Author(s): 小林主弥
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2009-09-23
• [Presentation] ガスセンサータンパク質HemATの酸素脱離に伴う構造変化と機能発現 (2009)
  • Author(s): 吉田祐
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2009-09-23
• [Presentation] 光反応中間体におけるイエロープロテインのタンパク質構造:発色団周辺水素結合変化の変異体紫外共鳴ラマン測定による解析 (2009)
  • Author(s): 水野操
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2009-09-22
• [Presentation] タンパク質の超高速過程:構造緩和とエネルギー緩和 (2009)
  • Author(s): 水谷泰久
  • Organizer: 特定領域研究「実在系の分子理論」研究交流会
  • Place of Presentation: 金沢文化ホール(石川県)
  • Year and Date: 2009-09-04
• [Presentation] Primary protein responses of after ligand dissociation and chromophore isomerization : time-resolved resonance Raman studies (2009)
  • Author(s): 水谷泰久
  • Organizer: Telluride Science Research Center Workshop on Protein Dynamics
  • Place of Presentation: Telluride Science Research Center(アメリカ、コロラド州)
  • Year and Date: 2009-08-05
• [Presentation] Structural dynamics of hemeproteins initiated by oxygen dissociation (2009)
  • Author(s): 水谷泰久
  • Organizer: Japan-Korea Symposium on Molecular Science 2009 "Chemical Dynamics in Materials and Biological Molecular Sciences"
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-07-13
• [Presentation] Protein Dynamics of HemAT upon Oxygen Dissociation as Revealed by Time-resolved Resonance Raman Spectroscopy (2009)
  • Author(s): 吉田祐
  • Organizer: Japan-Korea Symposium on Molecular Science 2009 "Chemical Dynamics in Materials and Biological Molecular Sciences"
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-07-12
• [Presentation] Picosecond dynamics of photoactive proteins upon the photoisomerization (2009)
  • Author(s): 水谷泰久
  • Organizer: International Symposium on "Reaction Dynamics of Many-Body Chemical Systems"
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2009-06-23
• [Presentation] 時間分解共鳴ラマン分光法によるガスセンサータンパク質HemATの酸素脱離に伴うダイナミクスの観測 (2009)
  • Author(s): 吉田祐
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-29
• [Presentation] バクテリオロドプシンの光反応初期過程におけるタンパク質ダイナミクス (2008)
  • Author(s): 水野操
  • Organizer: 第2回分子科学討論会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-09-24
• [Presentation] バクテリオロドプシンのピコ秒時間分解紫外共鳴ラマンスペクトル : 部位選択的観測でみるタンパク質部分の初期構造ダイナミクス (2008)
  • Author(s): 水野操
  • Organizer: 第35回生体分子科学討論会
  • Place of Presentation: 兵庫県立先端科学技術支援センター
  • Year and Date: 2008-06-27
• [Presentation] Hemoglobin allostery: dynamics and function (2008)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 28th Annual Pittsburgh Conference Lectures
  • Place of Presentation: University of Pittsburgh, USA.(招待講演)
  • Year and Date: 2008-04-16
• [Presentation] Hemoglobin allostery: dynamics and function (2008)
  • Author(s): Yasuhisa Mizutani
  • Organizer: 28th Annual Pittsburgh Conference Lectures
  • Place of Presentation: University of Pittsburgh, USA
  • Year and Date: 2008-04-16
• [Presentation] ヘムオキシゲナーゼーヘム複合体の-酸化炭素脱離に伴う構造ダイナミクス (2008)
  • Author(s): 山岡将人
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 立教大学
• [Presentation] Primary protein responses of myoglobin after ligand dissociation revealedby time-resolved resonance Raman spertroscopy (2007)
  • Author(s): Yasuhisa, Mizutain
  • Organizer: Telluride Science Resesarch Center Workshop on Protein Dynamics
  • Place of Presentation: Telluride, Colorado, USA
• [Presentation] タンパク質ダイナミクスと水素結合 (2007)
  • Author(s): 水谷泰久
  • Organizer: 北大低温研共同利用研究集会「低温凝縮系における水素の化学と物性」
  • Place of Presentation: 北海道大学
• [Presentation] 時間分解共鳴ラマン分光法で観たタンパク質ダイナミクス:ピコ秒からミリ秒まで、可視領域から紫外領域まで (2007)
  • Author(s): 水谷泰久
  • Organizer: 日本生物物理学会第45回年会シンポジウム「振動分光法が拓く生命科学;分子から細胞まで 」
  • Place of Presentation: パシフイコ横浜
• [Presentation] シリカゲル中に閉じ込めたヘモグロビンの構造ダイナミケス:ダイナミクスの四次構造依存性 (2007)
  • Author(s): 稲垣厚志
  • Organizer: 第1回分子科学討論会
  • Place of Presentation: 東北大学
• [Book] みず学への誘い, 88-97 (2008)
  • Author(s): 水谷泰久
  • Total Pages: 300
  • Publisher: 大阪大学出版社
• [Book] みず学への誘い (2007)
  • Author(s): 水谷泰久
  • Publisher: 大阪大学出版会
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/mizutani/index-jp.html