Elucidation of structural basis of dynamically-driven protein allostery by time-resolved resonance Raman spectroscopy
| Project Area | Molecular Science for Supra Functional Systems ? Development of Advanced Methods for Exploring Elementary Process |
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| Project/Area Number |
19056013
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Haruto 大阪大学, 大学院・理学研究科, 講師 (40551338)
MIZUNO Misao 大阪大学, 大学院・理学研究科, 助教 (10464257)
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| Project Period (FY) |
2007 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥69,200,000 (Direct Cost: ¥69,200,000)
Fiscal Year 2011: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 2009: ¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 2008: ¥29,700,000 (Direct Cost: ¥29,700,000)
Fiscal Year 2007: ¥16,800,000 (Direct Cost: ¥16,800,000)
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| Keywords | ラマン分光法 / 生物物理学 / 時間分解分光学 / 共鳴ラマン分光法 / 時間分解分光法 / アロステリックタンパク質 |
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| Research Abstract |
Protein dynamics highly relevant to biological function were studied with time-resolved resonance Raman spectroscopy. Our effort on developments on the measurement system enabled us to perform site-specific observation on structural changes of proteins in wide time range from picoseconds to milliseconds. We elucidated functionally-important dynamics of various allosteric proteins such as hemoglobins, heme-based gas sensors, and photoactive proteins with the high performance of the system.
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Report
(7 results)
Research Products
(72 results)
