Construction of normal cloned embryos and placentas by improvements of nuclear transfer technology in mice

• Project Area: Program of totipotency: From decoding to designing
• Project/Area Number: 19H05758
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ogura Atsuo 国立研究開発法人理化学研究所, バイオリソース研究センター, 室長 (20194524)
• Co-Investigator(Kenkyū-buntansha): 日野 敏昭 旭川医科大学, 医学部, 助教 (10550676)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥149,240,000 (Direct Cost: ¥114,800,000、Indirect Cost: ¥34,440,000); Fiscal Year 2023: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000); Fiscal Year 2022: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000); Fiscal Year 2021: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2020: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2019: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
• Keywords: 全能性 / ゲノム再プログラム化 / 核移植クローン / 受精 / 胎盤

Outline of Research at the Start

精子および卵子ゲノムは、受精により全能性を獲得する。この全能性の獲得には、卵子細胞質が大きく関わる。体細胞核移植は、その卵子細胞質中に存在する母性因子を利用して体細胞ゲノムに全能性を賦与する技術であるが、核移植クローン胚の多くは発生停止をしたり、あるいは生後の異常表現型を呈する。よって、正常な受精および体細胞核移植クローンにより得られた胚・胎仔・産子を詳細に解析することにより、完全な全能性獲得のための条件が明らかになると期待される。本研究では、発生工学的技術と最新のゲノム解析技術を駆使することにより、全能性獲得とその後の正常な発生・出生・成育に至るエピゲノム条件を明らかにする。

Outline of Final Research Achievements

Somatic cell cloning is a technique to endow the somatic cell genome with totipotency within the recipient ooplasm. We analyzed cloned embryos/placentas at the epigenome level to see how the genome acquires normal/abnormal reprogramming. We found that loss of H3K27me3-dependent imprinting occurred in cloned placentas, leading to placental enlargement at term and abnormal epithelial-mesenchymal transition (EMT) in the early placental tissues. We also found that treatment with a new G9a (histone methyltransferase) inhibitor significantly improved the cloning efficiency.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、核移植クローン技術は、エピゲノム修復原理に基づく格段の進歩につながった。その解析結果は、いかに全能性をもとに新しい生命が始まるかという生物学の最大の課題の一つに対し、解決の糸口を与えると期待される。また、クローン技術の進歩は、畜産・創薬などの産業応用（生物工場）、ヒト疾患モデルの開発（特に霊長類）、移植用臓器の作出、絶滅危惧種の保全などきわめて多くの応用へつながる。特に近年は、ブタを用いた異種移植用臓器の臨床利用が始まっており、その遺伝子改変ブタドナーの作成に必須となる核移植クローン技術の効率化は、将来の移植医療の普及に大きなインパクトを与えると期待される。

Research Products

• [Int'l Joint Research] University of South Florida(米国)
• [Int'l Joint Research] National Cancer Center(中国)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] University of South Florida/University of Hawaii(米国)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] 中国医学科学院(中国)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] Institute of Molecular Genetics, CAS(チェコ)
• [Int'l Joint Research] Inst Mol Genet, CAS(チェコ)
• [Int'l Joint Research] University of Hawaii/Harvard University(米国)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] Teramo University(イタリア)
• [Int'l Joint Research] 浙江省農業科学院(中国)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] Institute of Molecular Genetics(チェコ)
• [Int'l Joint Research] University of Hawaii/Harvard University(米国)
• [Int'l Joint Research] Teramo University(イタリア)
• [Journal Article] Inter-subspecies mouse F1 hybrid embryonic stem cell lines newly established for studies of allelic imbalance in gene expression (2024)
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  • Journal Title: Experimental Animals Volume: 73 Issue: 3 Pages: 310-318
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• [Journal Article] Effect of microgravity on mammalian embryo development evaluated at the International Space Station (2023)
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• [Journal Article] The fluid factor OVGP1 provides a significant oviductal microenvironment for the reproductive process in golden hamster (2023)
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  • Journal Title: Biology of Reproduction Volume: 110 Issue: 3 Pages: 465-475
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• [Journal Article] Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro. (2023)
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• [Journal Article] Editing the Genome of the Golden Hamster (Mesocricetus auratus) (2023)
  • Author(s): Hirose Michiko、Tomishima Toshiko、Ogura Atsuo
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• [Journal Article] SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. (2022)
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• [Journal Article] Suppression of endogenous retroviral enhancers in mouse embryos derived from somatic cell nuclear transfer (2022)
  • Author(s): Shikata Daiki、Matoba Shogo、Hada Masashi、Sakashita Akihiko、Inoue Kimiko、Ogura Atsuo
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• [Journal Article] Development of a new device for manipulating frozen mouse 2-cell embryos on the International Space Station (2022)
  • Author(s): Wakayama Sayaka、Soejima Mariko、Kikuchi Yasuyuki、Hayashi Erika、Ushigome Natsuki、Hasegawa Ayumi、Mochida Keiji、Suzuki Tomomi、Yamazaki Chiaki、Shimazu Toru、Sano Hiromi、Umehara Masumi、Matsunari Hitomi、Ogura Atsuo、Nagashima Hiroshi、Wakayama Teruhiko
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• [Journal Article] Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes (2022)
  • Author(s): Narumi Ogonuki, Hirohisa Kyogoku, Toshiaki Hino, Yuki Osawa, Yasuhiro Fujiwara, Kimiko Inoue, Tetsuo Kunieda, Seiya Mizuno, Hiroyuki Tateno, Fumihiro Sugiyama, Tomoya S. Kitajima, Atsuo Ogura
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• [Journal Article] Use of anti-inhibin monoclonal antibody for increasing the litter size of mouse strains and its application to <i>i</i>-GONAD (2022)
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  • Journal Title: Biology of Reproduction Volume: - Issue: 2 Pages: 605-618
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• [Journal Article] Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids (2022)
  • Author(s): Kanatsu-Shinohara Mito、Ogonuki Narumi、Matoba Shogo、Morimoto Hiroko、Shiromoto Yusuke、Ogura Atsuo、Shinohara Takashi
  • Journal Title: Stem Cell Reports Volume: 17 Issue: 4 Pages: 924-935
  • DOI: 10.1016/j.stemcr.2022.02.013
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• [Journal Article] Mouse <i>in vivo</i>-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and −80°C preservation than IVF or ICSI embryos (2022)
  • Author(s): HAYASHI Erika、WAKAYAMA Sayaka、ITO Daiyu、HASEGAWA Ayumi、MOCHIDA Keiji、OOGA Masatoshi、OGURA Atsuo、WAKAYAMA Teruhiko
  • Journal Title: Journal of Reproduction and Development Volume: 68 Issue: 2 Pages: 118-124
  • DOI: 10.1262/jrd.2021-115
  • NAID: 130008139601
  • ISSN: 0916-8818, 1348-4400
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• [Journal Article] Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells (2022)
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  • Journal Title: Genes Dev Volume: 36 Issue: 1-2 Pages: 84-102
  • DOI: 10.1101/gad.348782.121
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• [Journal Article] Spermatogonial stem cell transplantation into nonablated mouse recipient testes (2022)
  • Author(s): Hiroko Morimoto, Narumi Ogonuki, Mito Kanatsu-Shinohara , Shogo Matoba , Atsuo Ogura , Takashi Shinohara
  • Journal Title: Stem cell reports Volume: 16 Issue: 7 Pages: 1832-1844
  • DOI: 10.1016/j.stemcr.2021.05.013
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• [Journal Article] Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan (2021)
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  • Journal Title: Mammalian Genome Volume: 33 Issue: 1 Pages: 181-191
  • DOI: 10.1007/s00335-021-09916-x
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• [Journal Article] Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs (2021)
  • Author(s): Loubalova Zuzana、Fulka Helena、Horvat Filip、Pasulka Josef、Malik Radek、Hirose Michiko、Ogura Atsuo、Svoboda Petr
  • Journal Title: Nature Cell Biology Volume: 23 Issue: 9 Pages: 992-1001
  • DOI: 10.1038/s41556-021-00746-2
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• [Journal Article] Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors† (2021)
  • Author(s): Kamimura Satoshi、Inoue Kimiko、Mizutani Eiji、Kim Jin-Moon、Inoue Hiroki、Ogonuki Narumi、Miyamoto Kei、Ihashi Shunya、Itami Nobuhiko、Wakayama Teruhiko、Ito Akihiro、Nishino Norikazu、Yoshida Minoru、Ogura Atsuo
  • Journal Title: Biology of Reproduction Volume: 105 Issue: 2 Pages: 543-553
  • DOI: 10.1093/biolre/ioab096
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• [Journal Article] Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains (2021)
  • Author(s): Mochida Keiji、Hasegawa Ayumi、Shikata Daiki、Itami Nobuhiko、Hada Masashi、Watanabe Naomi、Tomishima Toshiko、Ogura Atsuo
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 14149-14149
  • DOI: 10.1038/s41598-021-93604-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice (2021)
  • Author(s): Akter Most Sumona、Hada Masashi、Shikata Daiki、Watanabe Gen、Ogura Atsuo、Matoba Shogo
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 15438-15438
  • DOI: 10.1038/s41598-021-94851-9
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• [Journal Article] Tsga8 is required for spermatid morphogenesis and male fertility in mice (2021)
  • Author(s): Kobayashi Yuki、Tomizawa Shin-ichi、Ono Michio、Kuroha Kazushige、Minamizawa Keisuke、Natsume Koji、Dizdarevic Selma、Dockal Ivana、Tanaka Hiromitsu、Kawagoe Tatsukata、Seki Masahide、Suzuki Yutaka、Ogonuki Narumi、Inoue Kimiko、Matoba Shogo、Anastassiadis Konstantinos、Mizuki Nobuhisa、Ogura Atsuo、Ohbo Kazuyuki
  • Journal Title: Development Volume: 148 Issue: 8
  • DOI: 10.1242/dev.196212
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture (2021)
  • Author(s): SUN Shuai、YANO Shota、NAKANISHI Momo O、HIROSE Michiko、NAKABAYASHI Kazuhiko、HATA Kenichiro、OGURA Atsuo、TANAKA Satoshi
  • Journal Title: Journal of Reproduction and Development Volume: 67 Issue: 3 Pages: 197-205
  • DOI: 10.1262/jrd.2020-119
  • NAID: 130008055012
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] OGG1 protects mouse spermatogonial stem cells from reactive oxygen species in culture (2021)
  • Author(s): Mori Y, Ogonuki N, Hasegawa A, Kanatsu-Shinohara M, Ogura A, Wang Y, McCarrey JR, Shinohara T.
  • Journal Title: Biol Reprod Volume: 104 Issue: 3 Pages: 706-716
  • DOI: 10.1093/biolre/ioaa216
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• [Journal Article] An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia. (2021)
  • Author(s): Hiroko Morimoto, Takuya Yamamoto, Takehiro Miyazaki, Harumi Ogonuki, Atsuo Ogura, Takashi Tanaka, Mito Kanatsu-Shinohara , Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier, Andreas Trumpp, Takashi Shinohara
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• [Journal Article] Reprogramming of the histone H3.3 landscape in the early mouse embryo. (2021)
  • Author(s): Ishiuchi, T., Abe, S., Inoue, K., Au Yeung, W.K., Miki, Y., Ogura, A. & Sasaki, H.
  • Journal Title: Nature Structural & Molecular Biology Volume: 28 Issue: 1 Pages: 38-49
  • DOI: 10.1038/s41594-020-00521-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epigenetic abnormalities associated with somatic cell nuclear transfer (2021)
  • Author(s): Ogura Atsuo、Matoba Shogo、Inoue Kimiko
  • Journal Title: Reproduction Volume: -
  • DOI: 10.1530/rep-21-0013
  • Peer Reviewed / Open Access
• [Journal Article] 配偶子形成を経ない体細胞クローンの解析から見えてくる経世代エピゲノムの重要性 (2021)
  • Author(s): 的場章悟，小倉淳郎
  • Journal Title: 実験医学 Volume: 39 Pages: 889-895
• [Journal Article] Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3† (2020)
  • Author(s): Miura Kento、Matoba Shogo、Hirose Michiko、Ogura Atsuo
  • Journal Title: Biology of Reproduction Volume: 104 Issue: 1 Pages: 223-233
  • DOI: 10.1093/biolre/ioaa176
  • NAID: 130007925789
  • Peer Reviewed / Open Access
• [Journal Article] Development of assisted reproductive technologies for Mus spretus (2020)
  • Author(s): Hasegawa Ayumi、Mochida Keiji、Matoba Shogo、Inoue Kimiko、Hama Daiki、Kadota Masayo、Hiraiwa Noriko、Yoshiki Atsushi、Ogura Atsuo
  • Journal Title: Biology of Reproduction Volume: 104 Issue: 1 Pages: 234-243
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  • Peer Reviewed / Open Access
• [Journal Article] Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency (2020)
  • Author(s): Tsuchimoto Akihiro、Tone Masaaki、Ogonuki Narumi、Hada Masashi、Ogura Atsuo、Takashima Seiji
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 10796-10796
  • DOI: 10.1038/s41598-020-67595-1
  • Peer Reviewed / Open Access
• [Journal Article] Loss of H3K27me3 imprinting in the Sfmbt2 miRNA cluster causes enlargement of cloned mouse placentas (2020)
  • Author(s): Inoue Kimiko、Ogonuki Narumi、Kamimura Satoshi、Inoue Hiroki、Matoba Shogo、Hirose Michiko、Honda Arata、Miura Kento、Hada Masashi、Hasegawa Ayumi、Watanabe Naomi、Dodo Yukiko、Mochida Keiji、Ogura Atsuo
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 2150-2150
  • DOI: 10.1038/s41467-020-16044-8
  • Peer Reviewed / Open Access
• [Journal Article] Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice (2020)
  • Author(s): Kanatsu-Shinohara Mito、Ogonuki Narumi、Matoba Shogo、Ogura Atsuo、Shinohara Takashi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 14 Pages: 7837-7844
  • DOI: 10.1073/pnas.1914963117
  • NAID: 120006826644
  • Peer Reviewed / Open Access
• [Journal Article] Acrosin is essential for sperm penetration through the zona pellucida in hamsters (2020)
  • Author(s): Hirose Michiko、Honda Arata、Fulka Helena、Tamura-Nakano Miwa、Matoba Shogo、Tomishima Toshiko、Mochida Keiji、Hasegawa Ayumi、Nagashima Kiyoshi、Inoue Kimiko、Ohtsuka Masato、Baba Tadashi、Yanagimachi Ryuzo、Ogura Atsuo
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 5 Pages: 2513-2518
  • DOI: 10.1073/pnas.1917595117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Birth of a marmoset following injection of elongated spermatid from a prepubertal male (2019)
  • Author(s): Ogonuki Narumi、Abe Yukiko、Kurotaki Yoko K.、Nakao Kazuki、Aiba Atsu、Sasaki Erika、Ogura Atsuo
  • Journal Title: Molecular Reproduction and Development Volume: 86 Issue: 8 Pages: 928-930
  • DOI: 10.1002/mrd.23220
  • NAID: 130007719226
  • Peer Reviewed
• [Journal Article] Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming (2019)
  • Author(s): FULKA Helena、OGURA Atsuo、LOI Pasqualino、FULKA, Jr. Josef
  • Journal Title: Journal of Reproduction and Development Volume: 65 Issue: 5 Pages: 433-441
  • DOI: 10.1262/jrd.2019-017
  • NAID: 130007732099
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Paternal knockout of Slc38a4/SNAT4 causes placental hypoplasia associated with intrauterine growth restriction in mice (2019)
  • Author(s): Matoba Shogo、Nakamuta Shoko、Miura Kento、Hirose Michiko、Shiura Hirosuke、Kohda Takashi、Nakamuta Nobuaki、Ogura Atsuo
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 42 Pages: 21047-21053
  • DOI: 10.1073/pnas.1907884116
  • NAID: 130007719356
  • Peer Reviewed / Open Access
• [Journal Article] Early production of offspring by <i>in vitro</i> fertilization using first-wave spermatozoa from prepubertal male mice (2019)
  • Author(s): MOCHIDA Keiji、HASEGAWA Ayumi、OGONUKI Narumi、INOUE Kimiko、OGURA Atsuo
  • Journal Title: Journal of Reproduction and Development Volume: 65 Issue: 5 Pages: 467-473
  • DOI: 10.1262/jrd.2019-042
  • NAID: 130007732105
  • Peer Reviewed / Open Access
• [Journal Article] Active peristaltic movements and fluid production of the mouse oviduct: Their roles in fluid and sperm transport and fertilization (2019)
  • Author(s): Toshiaki Hino, Ryuzo Yanagimachi
  • Journal Title: Biology of Reproduction Volume: 印刷中 Issue: 1 Pages: 40-49
  • DOI: 10.1093/biolre/ioz061
  • Peer Reviewed / Int'l Joint Research
• [Presentation] ハムスターから学ぶ新たな生殖生物学 (2024)
  • Author(s): 小倉淳郎
  • Organizer: 第4回有性生殖研究会
  • Invited
• [Presentation] 核移植技術を用いた野生由来マウスES細胞の樹立 (2023)
  • Author(s): 小倉淳郎
  • Organizer: 第33回モロシヌス研究会
  • Invited
• [Presentation] 小型実験動物の発生工学技術の開発およびその応用研究について (2023)
  • Author(s): 小倉淳郎
  • Organizer: 第45回 北海道大学獣医学学術交流基金群講演会
  • Invited
• [Presentation] Nuclear transfer for the study of the developmental epigenetics (2023)
  • Author(s): Atsuo Ogura
  • Organizer: The 36th International Mammalian Genome Conference
  • Int'l Joint Research / Invited
• [Presentation] 顕微鏡下で命をつくる ー 核移植クローンと顕微授精 ー (2022)
  • Author(s): 小倉淳郎
  • Organizer: 第12回日本マーモセット研究会
  • Invited
• [Presentation] How to improve mouse cloning (2022)
  • Author(s): Atsuo Ogura
  • Organizer: 19th International Congress on Animal Reproduction
  • Int'l Joint Research / Invited
• [Presentation] How somatic cell nuclear transfer helps us probe the placental epigenome (2022)
  • Author(s): Atsuo Ogura
  • Organizer: International Symposium "Totipotency and Germ Cell Development"
  • Int'l Joint Research / Invited
• [Presentation] 核移植クローンによる胎盤エピゲノムの特性解析 (2022)
  • Author(s): 小倉淳郎
  • Organizer: 大阪大学蛋白質研セミナー 生殖細胞・減数分裂研究の最前線
  • Invited
• [Presentation] 論文執筆・投稿からacceptまで～私の経験 (2021)
  • Author(s): 小倉淳郎
  • Organizer: 日本繁殖生物学会オンラインセミナー
  • Invited
• [Presentation] 小型実験動物を用いた受精・胚発生・胎盤発生機構の解明 (2021)
  • Author(s): 小倉淳郎
  • Organizer: 第5回HAC鹿児島
  • Invited
• [Presentation] ゲノム編集技術を用いたノックアウトハムスターの作出 (2021)
  • Author(s): 小倉淳郎、廣瀬美智子、冨島俊子
  • Organizer: 第94回日本生化学会
  • Invited
• [Presentation] Recent advancements of assisted fertilization using spermatogenic cells (2020)
  • Author(s): Ogura A, Ogonuki N
  • Organizer: The 43th Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research / Invited
• [Presentation] マウス体細胞クローンを用いた胎盤形成機構の解析 (2020)
  • Author(s): 小倉淳郎
  • Organizer: 第113回 日本繁殖生物学会大会
  • Invited
• [Presentation] What do we learn from somatic cell nuclear transfer (SCNT)? (2019)
  • Author(s): Atsuo Ogura
  • Organizer: 42nd Annual Meeting of the Molecular Biology Society of Japan
  • Int'l Joint Research / Invited
• [Presentation] 受精におけるヒストンアルギニンメチル化の役割 (2019)
  • Author(s): 小倉淳郎
  • Organizer: 第92回日本生化学会大会
  • Invited
• [Presentation] 実験動物を用いた生殖技術の開発とその応用 (2019)
  • Author(s): 小倉淳郎
  • Organizer: 第57回東北生殖医学会総会・学術講演会
  • Invited
• [Presentation] Amazingly active peristaltic movements and fluid production of the mouse oviduct: their roles in fluid and sperm transport and fertilization (2019)
  • Author(s): Toshiaki Hino
  • Organizer: 52nd annual conference, Society for the study of the reproduction
  • Int'l Joint Research
• [Book] 繁殖生物学改訂版 (2020)
  • Author(s): 小倉淳郎他
  • Total Pages: 368
  • Publisher: インターズー
  • ISBN: 9784866711102
• [Remarks] 遺伝工学基盤技術室
  • URL: https://kougaku.brc.riken.jp/ja/
• [Remarks] Bioresource Engineering Division
  • URL: https://kougaku.brc.riken.jp/en/
• [Remarks] 遺伝工学基盤技術室
  • URL: https://ja.brc.riken.jp/lab/kougaku/
• [Remarks] Bioresource Engineering Division
  • URL: https://ja.brc.riken.jp/lab/kougaku/indexE.html
• [Funded Workshop] International Symposium "Totipotency and Germ Cell Development" (2022)