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Construction of normal cloned embryos and placentas by improvements of nuclear transfer technology in mice

Planned Research

Project AreaProgram of totipotency: From decoding to designing
Project/Area Number 19H05758
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ogura Atsuo  国立研究開発法人理化学研究所, バイオリソース研究センター, 室長 (20194524)

Co-Investigator(Kenkyū-buntansha) 日野 敏昭  旭川医科大学, 医学部, 助教 (10550676)
Project Period (FY) 2019-06-28 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥149,240,000 (Direct Cost: ¥114,800,000、Indirect Cost: ¥34,440,000)
Fiscal Year 2023: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2022: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2021: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2020: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2019: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Keywords全能性 / ゲノム再プログラム化 / 核移植クローン / 受精 / 胎盤
Outline of Research at the Start

精子および卵子ゲノムは、受精により全能性を獲得する。この全能性の獲得には、卵子細胞質が大きく関わる。体細胞核移植は、その卵子細胞質中に存在する母性因子を利用して体細胞ゲノムに全能性を賦与する技術であるが、核移植クローン胚の多くは発生停止をしたり、あるいは生後の異常表現型を呈する。よって、正常な受精および体細胞核移植クローンにより得られた胚・胎仔・産子を詳細に解析することにより、完全な全能性獲得のための条件が明らかになると期待される。本研究では、発生工学的技術と最新のゲノム解析技術を駆使することにより、全能性獲得とその後の正常な発生・出生・成育に至るエピゲノム条件を明らかにする。

Outline of Final Research Achievements

Somatic cell cloning is a technique to endow the somatic cell genome with totipotency within the recipient ooplasm. We analyzed cloned embryos/placentas at the epigenome level to see how the genome acquires normal/abnormal reprogramming. We found that loss of H3K27me3-dependent imprinting occurred in cloned placentas, leading to placental enlargement at term and abnormal epithelial-mesenchymal transition (EMT) in the early placental tissues. We also found that treatment with a new G9a (histone methyltransferase) inhibitor significantly improved the cloning efficiency.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、核移植クローン技術は、エピゲノム修復原理に基づく格段の進歩につながった。その解析結果は、いかに全能性をもとに新しい生命が始まるかという生物学の最大の課題の一つに対し、解決の糸口を与えると期待される。また、クローン技術の進歩は、畜産・創薬などの産業応用(生物工場)、ヒト疾患モデルの開発(特に霊長類)、移植用臓器の作出、絶滅危惧種の保全などきわめて多くの応用へつながる。特に近年は、ブタを用いた異種移植用臓器の臨床利用が始まっており、その遺伝子改変ブタドナーの作成に必須となる核移植クローン技術の効率化は、将来の移植医療の普及に大きなインパクトを与えると期待される。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (81 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (17 results) Journal Article (41 results) (of which Int'l Joint Research: 15 results,  Peer Reviewed: 40 results,  Open Access: 34 results) Presentation (17 results) (of which Int'l Joint Research: 6 results,  Invited: 16 results) Book (1 results) Remarks (4 results) Funded Workshop (1 results)

  • [Int'l Joint Research] University of South Florida(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] National Cancer Center(中国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Teramo University(イタリア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of South Florida/University of Hawaii(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Teramo University(イタリア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 中国医学科学院(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Teramo University(イタリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Institute of Molecular Genetics, CAS(チェコ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Inst Mol Genet, CAS(チェコ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Hawaii/Harvard University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Teramo University(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 浙江省農業科学院(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Institute of Molecular Genetics(チェコ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Hawaii/Harvard University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Teramo University(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Inter-subspecies mouse F1 hybrid embryonic stem cell lines newly established for studies of allelic imbalance in gene expression2024

    • Author(s)
      SAITO Ayaka、TAHARA Ryosuke、HIROSE Michiko、KADOTA Masayo、HASEGAWA Ayumi、KONDO Shinji、KATO Hidemasa、AMANO Takanori、YOSHIKI Atsushi、OGURA Atsuo、KIYOSAWA Hidenori
    • Journal Title

      Experimental Animals

      Volume: 73 Issue: 3 Pages: 310-318

    • DOI

      10.1538/expanim.24-0002

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of microgravity on mammalian embryo development evaluated at the International Space Station2023

    • Author(s)
      Wakayama Sayaka、Kikuchi Yasuyuki、Soejima Mariko、Hayashi Erika、Ushigome Natsuki、Yamazaki Chiaki、Suzuki Tomomi、Shimazu Toru、Yamamori Tohru、Osada Ikuko、Sano Hiromi、Umehara Masumi、Hasegawa Ayumi、Mochida Keiji、Yang Li Ly、Emura Rina、Kazama Kousuke、Imase Kenta、Kurokawa Yuna et al.,
    • Journal Title

      iScience

      Volume: 26 Issue: 11 Pages: 108177-108177

    • DOI

      10.1016/j.isci.2023.108177

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The fluid factor OVGP1 provides a significant oviductal microenvironment for the reproductive process in golden hamster2023

    • Author(s)
      Yamatoya Kenji、Kurosawa Masaru、Hirose Michiko、Miura Yoshiki、Taka Hikari、Nakano Tomoyuki、Hasegawa Akiko、Kagami Kyosuke、Yoshitake Hiroshi、Goto Kaoru、Ueno Takashi、Fujiwara Hiroshi、Shinkai Yoichi、Kan Frederick W K、Ogura Atsuo、Araki Yoshihiko
    • Journal Title

      Biology of Reproduction

      Volume: 110 Issue: 3 Pages: 465-475

    • DOI

      10.1093/biolre/ioad159

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Incomplete activation of<i>Alyref</i>and<i>Gabpb1</i>leads to preimplantation arrest in cloned mouse embryos2023

    • Author(s)
      Ihashi Shunya、Hamanaka Mizuto、Kaji Masaya、Mori Ryunosuke、Nishizaki Shuntaro、Mori Miki、Imasato Yuma、Inoue Kimiko、Matoba Shogo、Ogonuki Narumi、Takasu Atsushi、Nakamura Misaki、Matsumoto Kazuya、Anzai Masayuki、Ogura Atsuo、Ikawa Masahito、Miyamoto Kei
    • Journal Title

      Life Science Alliance

      Volume: 6 Issue: 11 Pages: e202302296-e202302296

    • DOI

      10.26508/lsa.202302296

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism2023

    • Author(s)
      Shimazu Tadahiro、Yoshimoto Rei、Kotoshiba Kaoru、Suzuki Takehiro、Matoba Shogo、Hirose Michiko、Akakabe Mai、Sohtome Yoshihiro、Sodeoka Mikiko、Ogura Atsuo、Dohmae Naoshi、Shinkai Yoichi
    • Journal Title

      Genes & Development

      Volume: 37 Issue: 15-16 Pages: 724-742

    • DOI

      10.1101/gad.350755.123

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells2023

    • Author(s)
      Naomi Watanabe, Michiko Hirose, Ayumi Hasegawa, Keiji Mochida, Atsuo Ogura, Kimiko Inoue
    • Journal Title

      Scientific Report

      Volume: 13 Issue: 1 Pages: 11175-11175

    • DOI

      10.1038/s41598-023-38341-0

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SINE RNA of the imprinted miRNA clusters mediates constitutive type III interferon expression and antiviral protection in hemochorial placentas2023

    • Author(s)
      Wickramage Ishani、VanWye Jeffrey、Max Klaas、Lockhart John H.、Hortu Ismet、Mong Ezinne F.、Canfield John、Lamabadu Warnakulasuriya Patabendige Hiran M.、Guzeloglu-Kayisli Ozlem、Inoue Kimiko、Ogura Atsuo、Lockwood Charles J.、Akat Kemal M.、Tuschl Thomas、Kayisli Umit A.、Totary-Jain Hana
    • Journal Title

      Cell Host & Microbe

      Volume: 31 Issue: 7 Pages: 1185-1199.e10

    • DOI

      10.1016/j.chom.2023.05.018

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro.2023

    • Author(s)
      Miyazaki T, Kanatsu-Shinohara M, Ogonuki N, Matoba S, Ogura A, Yabe-Nishimura C, Zhang H, Pommier Y, Trumpp A, Shinohara T.
    • Journal Title

      Development

      Volume: 150 Issue: 20

    • DOI

      10.1242/dev.201157

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Editing the Genome of the Golden Hamster (Mesocricetus auratus)2023

    • Author(s)
      Hirose Michiko、Tomishima Toshiko、Ogura Atsuo
    • Journal Title

      Methods Mol Biol

      Volume: 2637 Pages: 247-254

    • DOI

      10.1007/978-1-0716-3016-7_19

    • ISBN
      9781071630150, 9781071630167
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse.2022

    • Author(s)
      Uchida, A., Imaimatsu, K., Suzuki, H., Han, X., Ushioda, H., Uemura, M., Imura-Kishi, K., Hiramatsu, R., Takase, H. M., Hirate, Y., Ogura, A., Kanai-Azuma, M., Kudo, A., & Kanai, Y.
    • Journal Title

      Nature communications

      Volume: 13 (1) Issue: 1 Pages: 7860-7860

    • DOI

      10.1038/s41467-022-35465-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppression of endogenous retroviral enhancers in mouse embryos derived from somatic cell nuclear transfer2022

    • Author(s)
      Shikata Daiki、Matoba Shogo、Hada Masashi、Sakashita Akihiko、Inoue Kimiko、Ogura Atsuo
    • Journal Title

      Frontiers in Genetics

      Volume: 13 Pages: 1032760-1032760

    • DOI

      10.3389/fgene.2022.1032760

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a new device for manipulating frozen mouse 2-cell embryos on the International Space Station2022

    • Author(s)
      Wakayama Sayaka、Soejima Mariko、Kikuchi Yasuyuki、Hayashi Erika、Ushigome Natsuki、Hasegawa Ayumi、Mochida Keiji、Suzuki Tomomi、Yamazaki Chiaki、Shimazu Toru、Sano Hiromi、Umehara Masumi、Matsunari Hitomi、Ogura Atsuo、Nagashima Hiroshi、Wakayama Teruhiko
    • Journal Title

      PLOS ONE

      Volume: 17 Issue: 10 Pages: e0270781-e0270781

    • DOI

      10.1371/journal.pone.0270781

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes2022

    • Author(s)
      Narumi Ogonuki, Hirohisa Kyogoku, Toshiaki Hino, Yuki Osawa, Yasuhiro Fujiwara, Kimiko Inoue, Tetsuo Kunieda, Seiya Mizuno, Hiroyuki Tateno, Fumihiro Sugiyama, Tomoya S. Kitajima, Atsuo Ogura
    • Journal Title

      EMBO Rep

      Volume: 5;23(7) Issue: 7

    • DOI

      10.15252/embr.202254992

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Use of anti-inhibin monoclonal antibody for increasing the litter size of mouse strains and its application to <i>i</i>-GONAD2022

    • Author(s)
      Hasegawa Ayumi、Mochida Keiji、Nakamura Ayaka、Miyagasako Rico、Ohtsuka Masato、Hatakeyama Masahiko、Ogura Atsuo
    • Journal Title

      Biology of Reproduction

      Volume: - Issue: 2 Pages: 605-618

    • DOI

      10.1093/biolre/ioac068

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids2022

    • Author(s)
      Kanatsu-Shinohara Mito、Ogonuki Narumi、Matoba Shogo、Morimoto Hiroko、Shiromoto Yusuke、Ogura Atsuo、Shinohara Takashi
    • Journal Title

      Stem Cell Reports

      Volume: 17 Issue: 4 Pages: 924-935

    • DOI

      10.1016/j.stemcr.2022.02.013

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mouse <i>in vivo</i>-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and −80°C preservation than IVF or ICSI embryos2022

    • Author(s)
      HAYASHI Erika、WAKAYAMA Sayaka、ITO Daiyu、HASEGAWA Ayumi、MOCHIDA Keiji、OOGA Masatoshi、OGURA Atsuo、WAKAYAMA Teruhiko
    • Journal Title

      Journal of Reproduction and Development

      Volume: 68 Issue: 2 Pages: 118-124

    • DOI

      10.1262/jrd.2021-115

    • NAID

      130008139601

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells2022

    • Author(s)
      Hada M, Miura H, Tanigawa A, Matoba S, Inoue K, Ogonuki N, Hirose M, Watanabe N, Nakato R, Fujiki K, Hasegawa A, Sakashita A, Okae H, Miura K, Shikata D, Arima T, Shirahige K, Hiratani I, Ogura A.
    • Journal Title

      Genes Dev

      Volume: 36 Issue: 1-2 Pages: 84-102

    • DOI

      10.1101/gad.348782.121

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spermatogonial stem cell transplantation into nonablated mouse recipient testes2022

    • Author(s)
      Hiroko Morimoto, Narumi Ogonuki, Mito Kanatsu-Shinohara , Shogo Matoba , Atsuo Ogura , Takashi Shinohara
    • Journal Title

      Stem cell reports

      Volume: 16 Issue: 7 Pages: 1832-1844

    • DOI

      10.1016/j.stemcr.2021.05.013

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan2021

    • Author(s)
      Mizuno-Iijima Saori、Nakashiba Toshiaki、Ayabe Shinya、Nakata Hatsumi、Ike Fumio、Hiraiwa Noriko、Mochida Keiji、Ogura Atsuo、Masuya Hiroshi、Kawamoto Shoko、Tamura Masaru、Obata Yuichi、Shiroishi Toshihiko、Yoshiki Atsushi
    • Journal Title

      Mammalian Genome

      Volume: 33 Issue: 1 Pages: 181-191

    • DOI

      10.1007/s00335-021-09916-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs2021

    • Author(s)
      Loubalova Zuzana、Fulka Helena、Horvat Filip、Pasulka Josef、Malik Radek、Hirose Michiko、Ogura Atsuo、Svoboda Petr
    • Journal Title

      Nature Cell Biology

      Volume: 23 Issue: 9 Pages: 992-1001

    • DOI

      10.1038/s41556-021-00746-2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improved development of mouse somatic cell nuclear transfer embryos by chlamydocin analogues, class I and IIa histone deacetylase inhibitors†2021

    • Author(s)
      Kamimura Satoshi、Inoue Kimiko、Mizutani Eiji、Kim Jin-Moon、Inoue Hiroki、Ogonuki Narumi、Miyamoto Kei、Ihashi Shunya、Itami Nobuhiko、Wakayama Teruhiko、Ito Akihiro、Nishino Norikazu、Yoshida Minoru、Ogura Atsuo
    • Journal Title

      Biology of Reproduction

      Volume: 105 Issue: 2 Pages: 543-553

    • DOI

      10.1093/biolre/ioab096

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains2021

    • Author(s)
      Mochida Keiji、Hasegawa Ayumi、Shikata Daiki、Itami Nobuhiko、Hada Masashi、Watanabe Naomi、Tomishima Toshiko、Ogura Atsuo
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 14149-14149

    • DOI

      10.1038/s41598-021-93604-y

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice2021

    • Author(s)
      Akter Most Sumona、Hada Masashi、Shikata Daiki、Watanabe Gen、Ogura Atsuo、Matoba Shogo
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 15438-15438

    • DOI

      10.1038/s41598-021-94851-9

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tsga8 is required for spermatid morphogenesis and male fertility in mice2021

    • Author(s)
      Kobayashi Yuki、Tomizawa Shin-ichi、Ono Michio、Kuroha Kazushige、Minamizawa Keisuke、Natsume Koji、Dizdarevic Selma、Dockal Ivana、Tanaka Hiromitsu、Kawagoe Tatsukata、Seki Masahide、Suzuki Yutaka、Ogonuki Narumi、Inoue Kimiko、Matoba Shogo、Anastassiadis Konstantinos、Mizuki Nobuhisa、Ogura Atsuo、Ohbo Kazuyuki
    • Journal Title

      Development

      Volume: 148 Issue: 8

    • DOI

      10.1242/dev.196212

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture2021

    • Author(s)
      SUN Shuai、YANO Shota、NAKANISHI Momo O、HIROSE Michiko、NAKABAYASHI Kazuhiko、HATA Kenichiro、OGURA Atsuo、TANAKA Satoshi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 67 Issue: 3 Pages: 197-205

    • DOI

      10.1262/jrd.2020-119

    • NAID

      130008055012

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] OGG1 protects mouse spermatogonial stem cells from reactive oxygen species in culture2021

    • Author(s)
      Mori Y, Ogonuki N, Hasegawa A, Kanatsu-Shinohara M, Ogura A, Wang Y, McCarrey JR, Shinohara T.
    • Journal Title

      Biol Reprod

      Volume: 104 Issue: 3 Pages: 706-716

    • DOI

      10.1093/biolre/ioaa216

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.2021

    • Author(s)
      Hiroko Morimoto, Takuya Yamamoto, Takehiro Miyazaki, Harumi Ogonuki, Atsuo Ogura, Takashi Tanaka, Mito Kanatsu-Shinohara , Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier, Andreas Trumpp, Takashi Shinohara
    • Journal Title

      Genes Dev.

      Volume: 35(3-4) Issue: 3-4 Pages: 250-260

    • DOI

      10.1101/gad.339903.120

    • NAID

      120006951673

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reprogramming of the histone H3.3 landscape in the early mouse embryo.2021

    • Author(s)
      Ishiuchi, T., Abe, S., Inoue, K., Au Yeung, W.K., Miki, Y., Ogura, A. & Sasaki, H.
    • Journal Title

      Nature Structural & Molecular Biology

      Volume: 28 Issue: 1 Pages: 38-49

    • DOI

      10.1038/s41594-020-00521-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epigenetic abnormalities associated with somatic cell nuclear transfer2021

    • Author(s)
      Ogura Atsuo、Matoba Shogo、Inoue Kimiko
    • Journal Title

      Reproduction

      Volume: -

    • DOI

      10.1530/rep-21-0013

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 配偶子形成を経ない体細胞クローンの解析から見えてくる経世代エピゲノムの重要性2021

    • Author(s)
      的場章悟,小倉淳郎
    • Journal Title

      実験医学

      Volume: 39 Pages: 889-895

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3†2020

    • Author(s)
      Miura Kento、Matoba Shogo、Hirose Michiko、Ogura Atsuo
    • Journal Title

      Biology of Reproduction

      Volume: 104 Issue: 1 Pages: 223-233

    • DOI

      10.1093/biolre/ioaa176

    • NAID

      130007925789

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of assisted reproductive technologies for Mus spretus2020

    • Author(s)
      Hasegawa Ayumi、Mochida Keiji、Matoba Shogo、Inoue Kimiko、Hama Daiki、Kadota Masayo、Hiraiwa Noriko、Yoshiki Atsushi、Ogura Atsuo
    • Journal Title

      Biology of Reproduction

      Volume: 104 Issue: 1 Pages: 234-243

    • DOI

      10.1093/biolre/ioaa177

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency2020

    • Author(s)
      Tsuchimoto Akihiro、Tone Masaaki、Ogonuki Narumi、Hada Masashi、Ogura Atsuo、Takashima Seiji
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 10796-10796

    • DOI

      10.1038/s41598-020-67595-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of H3K27me3 imprinting in the Sfmbt2 miRNA cluster causes enlargement of cloned mouse placentas2020

    • Author(s)
      Inoue Kimiko、Ogonuki Narumi、Kamimura Satoshi、Inoue Hiroki、Matoba Shogo、Hirose Michiko、Honda Arata、Miura Kento、Hada Masashi、Hasegawa Ayumi、Watanabe Naomi、Dodo Yukiko、Mochida Keiji、Ogura Atsuo
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 2150-2150

    • DOI

      10.1038/s41467-020-16044-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice2020

    • Author(s)
      Kanatsu-Shinohara Mito、Ogonuki Narumi、Matoba Shogo、Ogura Atsuo、Shinohara Takashi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 14 Pages: 7837-7844

    • DOI

      10.1073/pnas.1914963117

    • NAID

      120006826644

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acrosin is essential for sperm penetration through the zona pellucida in hamsters2020

    • Author(s)
      Hirose Michiko、Honda Arata、Fulka Helena、Tamura-Nakano Miwa、Matoba Shogo、Tomishima Toshiko、Mochida Keiji、Hasegawa Ayumi、Nagashima Kiyoshi、Inoue Kimiko、Ohtsuka Masato、Baba Tadashi、Yanagimachi Ryuzo、Ogura Atsuo
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 5 Pages: 2513-2518

    • DOI

      10.1073/pnas.1917595117

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Birth of a marmoset following injection of elongated spermatid from a prepubertal male2019

    • Author(s)
      Ogonuki Narumi、Abe Yukiko、Kurotaki Yoko K.、Nakao Kazuki、Aiba Atsu、Sasaki Erika、Ogura Atsuo
    • Journal Title

      Molecular Reproduction and Development

      Volume: 86 Issue: 8 Pages: 928-930

    • DOI

      10.1002/mrd.23220

    • NAID

      130007719226

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming2019

    • Author(s)
      FULKA Helena、OGURA Atsuo、LOI Pasqualino、FULKA, Jr. Josef
    • Journal Title

      Journal of Reproduction and Development

      Volume: 65 Issue: 5 Pages: 433-441

    • DOI

      10.1262/jrd.2019-017

    • NAID

      130007732099

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Paternal knockout of Slc38a4/SNAT4 causes placental hypoplasia associated with intrauterine growth restriction in mice2019

    • Author(s)
      Matoba Shogo、Nakamuta Shoko、Miura Kento、Hirose Michiko、Shiura Hirosuke、Kohda Takashi、Nakamuta Nobuaki、Ogura Atsuo
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 42 Pages: 21047-21053

    • DOI

      10.1073/pnas.1907884116

    • NAID

      130007719356

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Early production of offspring by <i>in vitro</i> fertilization using first-wave spermatozoa from prepubertal male mice2019

    • Author(s)
      MOCHIDA Keiji、HASEGAWA Ayumi、OGONUKI Narumi、INOUE Kimiko、OGURA Atsuo
    • Journal Title

      Journal of Reproduction and Development

      Volume: 65 Issue: 5 Pages: 467-473

    • DOI

      10.1262/jrd.2019-042

    • NAID

      130007732105

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Active peristaltic movements and fluid production of the mouse oviduct: Their roles in fluid and sperm transport and fertilization2019

    • Author(s)
      Toshiaki Hino, Ryuzo Yanagimachi
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中 Issue: 1 Pages: 40-49

    • DOI

      10.1093/biolre/ioz061

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ハムスターから学ぶ新たな生殖生物学2024

    • Author(s)
      小倉淳郎
    • Organizer
      第4回有性生殖研究会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 核移植技術を用いた野生由来マウスES細胞の樹立2023

    • Author(s)
      小倉淳郎
    • Organizer
      第33回モロシヌス研究会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 小型実験動物の発生工学技術の開発およびその応用研究について2023

    • Author(s)
      小倉淳郎
    • Organizer
      第45回 北海道大学獣医学学術交流基金群講演会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Nuclear transfer for the study of the developmental epigenetics2023

    • Author(s)
      Atsuo Ogura
    • Organizer
      The 36th International Mammalian Genome Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 顕微鏡下で命をつくる ー 核移植クローンと顕微授精 ー2022

    • Author(s)
      小倉淳郎
    • Organizer
      第12回日本マーモセット研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] How to improve mouse cloning2022

    • Author(s)
      Atsuo Ogura
    • Organizer
      19th International Congress on Animal Reproduction
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How somatic cell nuclear transfer helps us probe the placental epigenome2022

    • Author(s)
      Atsuo Ogura
    • Organizer
      International Symposium "Totipotency and Germ Cell Development"
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 核移植クローンによる胎盤エピゲノムの特性解析2022

    • Author(s)
      小倉淳郎
    • Organizer
      大阪大学蛋白質研セミナー 生殖細胞・減数分裂研究の最前線
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 論文執筆・投稿からacceptまで~私の経験2021

    • Author(s)
      小倉淳郎
    • Organizer
      日本繁殖生物学会オンラインセミナー
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 小型実験動物を用いた受精・胚発生・胎盤発生機構の解明2021

    • Author(s)
      小倉淳郎
    • Organizer
      第5回HAC鹿児島
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集技術を用いたノックアウトハムスターの作出2021

    • Author(s)
      小倉淳郎、廣瀬美智子、冨島俊子
    • Organizer
      第94回日本生化学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Recent advancements of assisted fertilization using spermatogenic cells2020

    • Author(s)
      Ogura A, Ogonuki N
    • Organizer
      The 43th Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス体細胞クローンを用いた胎盤形成機構の解析2020

    • Author(s)
      小倉淳郎
    • Organizer
      第113回 日本繁殖生物学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] What do we learn from somatic cell nuclear transfer (SCNT)?2019

    • Author(s)
      Atsuo Ogura
    • Organizer
      42nd Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 受精におけるヒストンアルギニンメチル化の役割2019

    • Author(s)
      小倉淳郎
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 実験動物を用いた生殖技術の開発とその応用2019

    • Author(s)
      小倉淳郎
    • Organizer
      第57回東北生殖医学会総会・学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Amazingly active peristaltic movements and fluid production of the mouse oviduct: their roles in fluid and sperm transport and fertilization2019

    • Author(s)
      Toshiaki Hino
    • Organizer
      52nd annual conference, Society for the study of the reproduction
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Book] 繁殖生物学改訂版2020

    • Author(s)
      小倉淳郎他
    • Total Pages
      368
    • Publisher
      インターズー
    • ISBN
      9784866711102
    • Related Report
      2019 Annual Research Report
  • [Remarks] 遺伝工学基盤技術室

    • URL

      https://kougaku.brc.riken.jp/ja/

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] Bioresource Engineering Division

    • URL

      https://kougaku.brc.riken.jp/en/

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 遺伝工学基盤技術室

    • URL

      https://ja.brc.riken.jp/lab/kougaku/

    • Related Report
      2019 Annual Research Report
  • [Remarks] Bioresource Engineering Division

    • URL

      https://ja.brc.riken.jp/lab/kougaku/indexE.html

    • Related Report
      2019 Annual Research Report
  • [Funded Workshop] International Symposium "Totipotency and Germ Cell Development"2022

    • Related Report
      2022 Annual Research Report

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Published: 2019-07-04   Modified: 2025-01-30  

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