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Induction of non-gametic embryo derived from the synergid cell.

Planned Research

Project AreaRemodeling Plant Reproduction System by Cell Fate Manipulations.
Project/Area Number 20H05781
Research Category

Grant-in-Aid for Transformative Research Areas (B)

Allocation TypeSingle-year Grants
Review Section Transformative Research Areas, Section (III)
Research InstitutionYokohama City University

Principal Investigator

Maruyama Daisuke  横浜市立大学, 木原生物学研究所, 准教授 (80724111)

Project Period (FY) 2020-10-02 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000)
Fiscal Year 2022: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2021: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2020: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Keywords重複受精 / 花粉管 / 助細胞 / 多胚性種子 / 助細胞胚 / 助細胞胚乳融合 / 多胚種子 / プログラム細胞死 / アポミクシス / 胚乳 / 細胞融合 / 体細胞 / 卵細胞
Outline of Research at the Start

被子植物の卵細胞の隣には、花粉管の誘引を担う助細胞が存在する。シロイヌナズナでは受精後に発達する種子の中で、役目を終えた助細胞は胚乳と細胞融合することで消滅する。ところが、イネのある系統では助細胞が消滅せずにそのまま発達して多胚種子が形成される。興味深いことに、われわれが分離した助細胞の細胞融合が起こらないシロイヌナズナの変異体でも、発達中の種子にある助細胞が胚の側で伸長する様子が観察された。本研究では、細胞融合によるプログラム細胞死を止めた助細胞の胚へ運命転換させる試みを通じ、生殖細胞の一種である助細胞や卵細胞、そして体細胞である胚を分ける鍵要素の解明を目指す。

Outline of Final Research Achievements

In flowering plants, two female gametophytic accessory cells play pivotal roles in pollen tube guidance and double fertilization. In most species, the synergid cells are thought to be eliminated after double fertilization, however, a few exceptions reported polyembryony due to the production of synergid-derived embryo. I thus tried to generate polyembryonic Arabidopsis by preventing synergid cell death and ectopic expression of transcription factor related to the somatic embryogenesis. Although polyembryony was not observed in the synergid elimination mutants expressing embryo-inducible RKD1 transcription factor in the synergid cell, this study illuminated next step toward formation of the synergid-derived embryo and brought novel insight on molecular mechanism of non-gametic cell fusion in plants that occurs in inactivating synergid cell after double fertilization.

Academic Significance and Societal Importance of the Research Achievements

卵細胞の姉妹細胞にあたる助細胞から胚を作出することは、近年需要が高まっているDouble Haploid(DH)技術、すなわち、半数体を介して全ての遺伝子をホモ接合とする形質固定法の開発に貢献する。本研究結果は今後の助細胞胚の作出に役立つと期待される。また、助細胞不活性化のツールとしての位置づけであった助細胞と胚乳の細胞融合機構については、融合制御因子であるCTL17の同定や機能解析を通じて多様な知見が得られた。この結果は被子植物の体づくりの可塑性を新たな視点から考えさせるような研究に繋がる発見といえる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (22 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 8 results) Presentation (7 results) (of which Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] ケンタッキー大学(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ケンタッキー大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] ケンタッキー大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Removal of the endoplasma membrane upon sperm cell activation after pollen tube discharge2023

    • Author(s)
      Sugi Naoya、Izumi Rie、Tomomi Shun、Susaki Daichi、Kinoshita Tetsu、Maruyama Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1116289-1116289

    • DOI

      10.3389/fpls.2023.1116289

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development2023

    • Author(s)
      Zhang Yilin、Maruyama Daisuke、Toda Erika、Kinoshita Atsuko、Okamoto Takashi、Mitsuda Nobutaka、Takasaki Hironori、Ohme‐Takagi Masaru
    • Journal Title

      FEBS Letters

      Volume: 597 Issue: 3 Pages: 407-417

    • DOI

      10.1002/1873-3468.14570

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring Novel Polytubey Reproduction Pathways Utilizing Cumulative Genetic Tools2023

    • Author(s)
      Sugi Naoya、Maruyama Daisuke
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 5 Pages: 454-460

    • DOI

      10.1093/pcp/pcad021

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible molecular mechanisms of persistent pollen tube growth without de novo transcription2022

    • Author(s)
      Motomura Kazuki、Sugi Naoya、Takeda Atsushi、Yamaoka Shohei、Maruyama Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1020306-1020306

    • DOI

      10.3389/fpls.2022.1020306

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A way to discover a hidden pollen-tube potential controlling directional tipgrowth in <i>Arabidopsis thaliana</i>.2022

    • Author(s)
      Motomura Kazuki、Maruyama Daisuke
    • Journal Title

      PLANT MORPHOLOGY

      Volume: 34 Issue: 1 Pages: 69-76

    • DOI

      10.5685/plmorphol.34.69

    • ISSN
      0918-9726, 1884-4154
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elongation of Siliques Without Pollination 3 Regulates Nutrient Flow Necessary for Embryogenesis2022

    • Author(s)
      Takasaki Hironori、Ikeda Miho、Hasegawa Reika、Zhang Yilin、Sakamoto Shingo、Maruyama Daisuke、Mitsuda Nobutaka、Kinoshita Tetsu、Ohme-Takagi Masaru
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 1 Pages: 117-123

    • DOI

      10.1093/pcp/pcac151

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells2022

    • Author(s)
      Susaki Daichi、Izumi Rie、Oi Takao、Takeuchi Hidenori、Shin Ji Min、Sugi Naoya、Kinoshita Tetsu、Higashiyama Tetsuya、Kawashima Tomokazu、Maruyama Daisuke
    • Journal Title

      The Plant Cell

      Volume: 35 Issue: 4 Pages: 1222-1240

    • DOI

      10.1093/plcell/koac371

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex2021

    • Author(s)
      Motomura Kazuki、Takeuchi Hidenori、Notaguchi Michitaka、Tsuchi Haruna、Takeda Atsushi、Kinoshita Tetsu、Higashiyama Tetsuya、Maruyama Daisuke
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2331-2331

    • DOI

      10.1038/s41467-021-22661-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis2021

    • Author(s)
      Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
    • Journal Title

      PLOS Biology

      Volume: 19 Issue: 3 Pages: 3001123-3001123

    • DOI

      10.1371/journal.pbio.3001123

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis GEX1 Is a Nuclear Membrane Protein of Gametes Required for Nuclear Fusion During Reproduction2020

    • Author(s)
      Nishikawa Shuh-ichi、Yamaguchi Yuki、Suzuki Chiharu、Yabe Ayaka、Sato Yuzuru、Kurihara Daisuke、Sato Yoshikatsu、Susaki Daichi、Higashiyama Tetsuya、Maruyama Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 548032-548032

    • DOI

      10.3389/fpls.2020.548032

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物受精の動態をライブイメージングで観察する2023

    • Author(s)
      丸山大輔
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナの非配偶子性の細胞融合の意義を理解する2022

    • Author(s)
      丸山大輔
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナ花粉管は先端から核を排除した状態でも伸長制御能力を保持している2022

    • Author(s)
      元村 一基,杉 直也,松本 歩,武内 秀憲,野田口 理孝,東山 哲也,木下 哲,山岡 尚平,竹田 篤史,丸山 大輔
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 受精依存的に発現するシロイヌナズナの細胞融合因子の同定2021

    • Author(s)
      丸山大輔
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナの生殖に伴う非配偶子融合の意義2021

    • Author(s)
      丸山 大輔,須﨑 大地,太田 かおる,木下 哲
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Presentation] 死を回避した助細胞は新たな運命を手に入れられるか2021

    • Author(s)
      丸山大輔
    • Organizer
      第62回日本植物生理学会年会・関連集会「植物生殖改変ワークショップ」
    • Related Report
      2020 Annual Research Report
  • [Presentation] 助細胞の細胞融合現象に異常を示すシロイヌナズナ変異体の解析2021

    • Author(s)
      丸山大輔、太田かおる、須﨑大地、木下哲
    • Organizer
      日本植物学会第 84回大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] 植物の精細胞が「一皮むけた」瞬間を撮影 ~重複受精の精巧な仕組みの一端を明らかに~

    • URL

      https://www.yokohama-cu.ac.jp/news/2022/20230131maruyama.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] アクチン繊維が花粉管の誘引を制御する ~助細胞による誘引ペプチド分泌のメカニズムを解明~

    • URL

      https://www.yokohama-cu.ac.jp/news/2022/202301susaki.html

    • Related Report
      2022 Annual Research Report

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Published: 2020-10-30   Modified: 2024-01-30  

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