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Sequential synthesis of functional synthetic nucleic acids

Planned Research

Project AreaOrganic Synthesis based on Integration of Chemical Reactions. New Methodologies and New Materials
Project/Area Number 21106007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTokyo Institute of Technology

Principal Investigator

SEKINE MITSUO  東京工業大学, 生命理工学研究科, 教授 (40111679)

Project Period (FY) 2009-07-23 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords連続合成 / マイクロフロー / 固相合成法 / U1snRNA / 2'-O-MCE-RNA / muscular dystrophy / trimethylguanosine cap / RNA transcript / 集積合成 / TMGキャップ構造 / 筋ジストロフィー / 核酸医薬 / 連続的固相合成 / 三重鎖形成核酸 / アンチジーン法 / マイクロフロー系反応 / 固相合成 / エキソン・スキッピング / 筋ジストロフィー医薬 / 人工核酸 / ホスホロチオエート / 3重鎖形成核酸 / 修飾RNA合成 / マイクロフロー合成 / 相間移動触媒反応 / 塩基対形成能 / 塩基識別能 / Activator 42 / 分子動力学的解析 / 塩素対形成能 / 分子設計 / 2重鎖熱融解曲線 / アミダイトユニット / 修飾塩基 / 水素結合
Research Abstract

In this study, we suceeded in synthesizing the full structure of U1snRNA, which was very difficult to synthesize, by using sequential condensations in the solid-phase. This 5'-terminal 2,2,7-trimethylguanosine cap structure has a function capable of passing through the nuclear membrane from the cytoplasm. The full structure of U1snRNA was constructed for the first time in the world by ligation with an RNA transcript. We also studied the synthesis of 2'-O-MCE-RNA phosphorothioates which have proved to be promising as drugs for treatment of muscular dystrophy. They could be obtained in good yields by using the optimized conditions. A uridine derivative required for this molecule was successfully synthesized by a microflow system.

Report

(6 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (102 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (35 results) (of which Peer Reviewed: 35 results) Presentation (65 results) (of which Invited: 7 results) Book (1 results) Remarks (1 results)

  • [Journal Article] A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds2014

    • Author(s)
      Yamada, K.; Masaki, Y.; Tsunoda, H.; Ohkubo, A.; Seio, K.;Sekine, M
    • Journal Title

      Org. Biomol. Chem

      Volume: 12 Pages: 2255-2262

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Properties of 5-and/or 2-modified 2'-O-cyanoethyl uridine residue: 2'-O-cyanoethyl-5-propynyl-2-thiouri dine as an efficient duplex stabilizing component2014

    • Author(s)
      Masaki, Y.; Miyasaka, R.;Hirai, K.;Kanamori, T.; Tsunoda, H.; Ohkubo, A.; Seio, K.; Sekine, M
    • Journal Title

      Org. Biomol. Chem

      Volume: 12 Pages: 1157-1162

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Properties of 5- and/or 2-modified 2'-O-cyanoethyl uridine residue: 2'-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component.2014

    • Author(s)
      Yamada K, Masaki Y, Tsunoda H, Ohkubo A, Seio K, Sekine M.
    • Journal Title

      Org Biomol Chem.

      Volume: 12 Issue: 14 Pages: 1157-62

    • DOI

      10.1039/c3ob42420k

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3'-terminus2013

    • Author(s)
      Iijima, Y.;Kojima, S.; Kodama, E.; Kurohagi, S.; Kanamori, T.; Masaki, Y.; Ohkubo, A.; Sekine, M.;Seio, K
    • Journal Title

      Org. Biomol. Chem

      Volume: 11 Pages: 8276-8282

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Synthesis of UlsnRNA Derivatives2013

    • Author(s)
      Ohkubo, A.; Kondo, Y.; Suzuki, M.;Kobayashi, H.;Kanamori, T.; Masaki, Y.; Seio, K.; Nagai, K.; Sekine, M
    • Journal Title

      Org. Lett

      Volume: 15 Issue: 17 Pages: 4386-4389

    • DOI

      10.1021/ol401917r

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine2013

    • Author(s)
      Seio, K.; Kanamori, T.;Tokugawa, M.;Ohzeki, H.;Masaki, Y.; Tsunoda, H.; Ohkubo, A.; Sekine, M. Bioorg
    • Journal Title

      Med. Chem

      Volume: 21 Pages: 3197-3201

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides2013

    • Author(s)
      Yamada, K.;Ohkubo, A.; Esaka, Y.; Kanamori, T.;Masaki, Y.;Seio, K.;Sekine, M. Bioorg
    • Journal Title

      Med. Chem. Lett

      Volume: 23 Pages: 3448-3451

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups2013

    • Author(s)
      Yamada, K.; Hattori, Y.; Inde, T.; Kanamori, T.; Ohkubo, A.; Seio, K.; Sekine, M. Bioorg
    • Journal Title

      Med. Chem. Lett

      Volume: 23 Pages: 776-778

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides.2013

    • Author(s)
      Yamada K, Ohkubo A, Esaka Y, Kanamori T, Masaki Y, Seio K, Sekine M.
    • Journal Title

      Bioorg Med Chem Lett.

      Volume: 23 Issue: 11 Pages: 3448-51

    • DOI

      10.1016/j.bmcl.2013.03.054

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups.2013

    • Author(s)
      Yamada K, Hattori Y, Inde T, Kanamori T, Ohkubo A, Seio K, Sekine M.
    • Journal Title

      Bioorg Med Chem Lett.

      Volume: 23 Issue: 3 Pages: 776-8

    • DOI

      10.1016/j.bmcl.2012.11.079

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of the stability of modified RNA duplexes based on deformability analysis: 0ligoribonucleotide derivatives modified with 2'O-cyanoethyl- 5-propynyl-2-thiouridine as a promising component2012

    • Author(s)
      Masaki, Y.; Miyasaka, R.;Hirai, K.;Tsunoda, H.; Ohkubo, A.;Seio, K.;Sekine, M
    • Journal Title

      Chem. Commun

      Volume: 48 Issue: 58 Pages: 7313-7315

    • DOI

      10.1039/c2cc33409g

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Properties of Cationic 2'O-[N-(4-aminobutyl)carbamoyl]- modified Oligonucleotides2012

    • Author(s)
      Seio, K.; Tokugawa, M.; Kanamori, T.; Tsunoda, H.;Ohkubo, A.;Sekine, M. Bioorg
    • Journal Title

      Med. Chem. Lett

      Volume: 22 Pages: 2470-2473

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of an Efficient Method for Phosphorodiamidate Bond Formation by Using Inorganic Salts2012

    • Author(s)
      Harakawa, T.; Tsunoda, H.; Ohkubo, A.;Seio, K.;Sekine, M. Bioorg
    • Journal Title

      Med. Chem. Lett

      Volume: 22 Pages: 1445-1447

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Short-RNA Selective Binding of Oligonucleotides Modified Using Adenosine and Guanosine Derivatives That Possess Cyclohexyl Phosphates as Substituents2012

    • Author(s)
      Seio, K.; Kurohagi, S.;Kodama, E.;Tsunoda, H.;Ohkubo, A.; Sekine, M
    • Journal Title

      Org. Biomol. Chem

      Volume: 10 Pages: 994-1006

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA Duplexes and Triplex-Forming Oligodeoxynucleotides Incorporating Modified Nucleosides Forming Stable and Selective Triplexes2012

    • Author(s)
      T.; Masaki, Y.; Mizuta, M.; Tsunoda, H.;Ohkubo, A.;Sekine, M.;Seio, K
    • Journal Title

      Org. Biomol. Chem

      Volume: 10 Pages: 1007-1013

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation of New Base Pairs between Inosine and 5-Methyl-2-thiocytidine Derivatives2012

    • Author(s)
      Ohkubo, A.;Nishino, Y.; Ito, Y.;Tsunoda, H.;Seio, K.;Sekine, M
    • Journal Title

      Org. Biomol. Chem

      Volume: 10 Pages: 2008-2010

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of 5'-Terminal Capped Oligonucleotides Using O-N Phosphoryl Migration of Phosphoramidite Derivatives2012

    • Author(s)
      Ohkubo, A.;Tago, N.;Nishino, Y.;Yamada, K.; Tsunoda, H.:Seio, K.:Sekine, M
    • Journal Title

      Org. Lett

      Volume: 14 Issue: 1 Pages: 10-13

    • DOI

      10.1021/ol2026075

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and properties of cationic 2' -O-[N-(4-aminobutyl)carbamoyl]modified oligonucleotides2012

    • Author(s)
      Seio, K.; Tokugawa, M.; Kanamori, T.; Tsunoda, H.; Ohkubo, A.; Sekine, M.
    • Journal Title

      Bioorg. Med. Chem. Lett

      Volume: 22 Issue: 7 Pages: 2470-2473

    • DOI

      10.1016/j.bmcl.2012.02.012

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts2012

    • Author(s)
      Harakawa,Taro
    • Journal Title

      BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

      Volume: 22 Pages: 1445-1447

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of 5'-Terminal Capped Oligonucleotides Using O-N Phosphoryl Migration of Phosphoramidite Derivatives2012

    • Author(s)
      Ohkubo,Akihiro
    • Journal Title

      ORGANIC LETTERS

      Volume: 14 Pages: 10-13

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Properties of Cationic 2'-O-[N-(4-aminobutyl)carbamoyl]modified Oligonucleotides2012

    • Author(s)
      Seio, K.; Tokugawa, M.; Kanamori, T.; Tsunoda, H.; Ohkubo, A.; Sekine, M
    • Journal Title

      Bioorg.Med.Chem.Lett

      Volume: 22 Pages: 2470-2473

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Duplexes and Triplex-Forming Oligodeoxynucleotides Incorporating Modified Nucleosides Forming stable and Selective Triplexes2012

    • Author(s)
      Kanamori, T.; Masaki, Y.; Mizuta, M.; Tsunoda, H.; Ohkubo, A.; Sekine, M.; Seio, K.
    • Journal Title

      Org.Biomol.Chem

      Volume: 10 Issue: 5 Pages: 1007-1013

    • DOI

      10.1039/c1ob06411h

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of New Base Pairs between Inosine and 5-Methyl-2-thiocytidine Derivatives2012

    • Author(s)
      Ohkubo, A.; Nishino, Y.; Ito, Y.; Tsunoda, H.; Seio, K.; Sekine
    • Journal Title

      Org.Biomol.Chem.

      Volume: 10 Issue: 10 Pages: 2008-2010

    • DOI

      10.1039/c2ob06641f

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Properties of Oligoribonucleotides Having 5'-Terminal 2,2,7-Trimethylguanosine Cap Analogues2011

    • Author(s)
      Ohkubo, A.;Yokouchi, A.;Yamada, K.;Tsunoda, H.;Seio, K.; Sekine, M
    • Journal Title

      Nucleic Acids Therapeutics

      Volume: 21 Issue: 5 Pages: A1-A64

    • DOI

      10.1089/nat.2011.1502

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stable Triplex Formation Using the Strong Stacking Effect of Consecutive Thionucleoside Moieties Incorporated into Triplex-forming Oligonucleotides2011

    • Author(s)
      Ohkubo, A.; Nishino, Y.; Yokouchi, A.; Ito, Y.; Noma, Y.;Tsunoda, H.;Sekine, M
    • Journal Title

      Chem. Commun

      Volume: 47 Pages: 12556-12558

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Hybridization Properties of 2'-O-Methylated Oligoribonucleotides Incorporating 2'-O-Naphthyluridines2011

    • Author(s)
      M.Sekine, et al.
    • Journal Title

      Org.Biomol.Chem.

      Volume: 76 Pages: 210-218

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of 2'-O-[2-(N-Methylcarbamoy1)ethyl]ribonucleosides Using Oxa-Michael Reaction and Chemical and Biological Properties of Oligonucleotide Derivatives Incorporating These Modified Ribonucleosides2011

    • Author(s)
      T.Yamada, et al.
    • Journal Title

      J.Org.Chem.

      Volume: 9(in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism2010

    • Author(s)
      Y.Masaki, et al.
    • Journal Title

      J.Phys.Chem.(B)

      Volume: 114 Pages: 2517-2524

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Linear Relationship between Deformability and Thermal Stability of 2'-O-Modified RNA Hetero Duplexes2010

    • Author(s)
      Y.Masaki, R.Miyasaka, A.Ohkubo, K.Seio, M.Sekine
    • Journal Title

      J.Phys.Cheer.(B) 114

      Pages: 2517-2524

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of 3'-Terminal Nucleosides Having a Silyl Linker into Polymer Supports without Base Protection2009

    • Author(s)
      A.Ohkubo, Y.Noma, K.Aoki, H.Tsunoda, K.Seio, M.Sekine
    • Journal Title

      J.Org.Chem. 74

      Pages: 2817-2823

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs2009

    • Author(s)
      K.Seio, Y.Takaku, K.Miyazaki, Y.Masaki, A.Ohkubo, M.Sekine
    • Journal Title

      Org.Biomol.Chem. 7

      Pages: 2440-2451

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient solid-phase synthesis of oligodeoxynucleotides having a 5'-terminal 2,2,7-trimethylguanosine pyrophosphate linkage2009

    • Author(s)
      A.Ohkubo, K.Sasaki, Y.Noma, H.Tsunoda, K.Seio, M.Sekine
    • Journal Title

      Bioorg.Med.Chem. 17

      Pages: 4819-4824

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Properties of Nucleoside Derivatives Masked by the Chemically Stable 2-(Trimethylsilyl)benzoyl Group2009

    • Author(s)
      K.Yamada, H.Taguchi, A.Ohkuho, K.Seio, M.Sekine
    • Journal Title

      Bioorg.Med.Chem. 17

      Pages: 5928-5932

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and hybridization of 2'-O-methyl-RNAs incorporating 2'-O-carbamoyluridine and unique participation of the carbamoyl group in U-G base pair2009

    • Author(s)
      K.Seio, R.Tawarada, T.Sasami, M.Serizawa, M.Ise, A.Ohkubo, M.Sekine
    • Journal Title

      Bioorg.Med.Chem. 17

      Pages: 7275-7280

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microwave-Assisted Synthesis of 2'-O-Aryluridine Derivatives2009

    • Author(s)
      Y.Oeda, Y.Iijima, H.Taguchi, A.Ohkubo, K.Seio, M.Sekine
    • Journal Title

      Org.Lett. 11

      Pages: 5582-5585

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3-アミノピリダジン型核酸塩基を有するペプチド核酸の合成と性質2014

    • Author(s)
      友利貴人,宮武佑弥,佐藤祐太,金森功吏,正木慶昭,大窪章寛,関根光雄,清尾康志
    • Organizer
      第94春季年会プログラム
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 筋ジストロフィー治療を指向した塩基部修飾2'-0-MCE RNAの合成と性質2014

    • Author(s)
      石井陽大,正木慶昭,山田剛史,岡庭夏己,永田哲也,武田伸一,金森功吏,大窪章寛,清尾康志,関根光雄
    • Organizer
      第94回春季年会プログラム
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] GFPクロモフォアを導入したDNA三重鎖の蛍光増強を目指した構造探索2014

    • Author(s)
      金森功吏,高村亮宏,正木慶昭,大窪章寛,関根光 雄,清尾康志
    • Organizer
      第94春季年会プログラム
    • Place of Presentation
      名古屋大学 東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] デオキシシュードイソシチジンと5-メチル -4-ピリミジノンヌクレオシドを導入したDNA 三重鎖の塩基対形成能の評価2014

    • Author(s)
      山口慧, 金森功吏,正木慶昭,大窪章寛,関根光 雄,清尾康志
    • Organizer
      第94回春季年会プログラム
    • Place of Presentation
      名古屋大学, 東山キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 筋ジストロフィー治療を指向した塩基部修飾2´-O-MCE RNAの合成と性質2014

    • Author(s)
      石井 陽大
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 塩基部修飾されたウリジンを含むホスホロチオエートオリゴヌクレオチドの合成とエキソンスキッピング活性2014

    • Author(s)
      印出健志
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] デオキシシュードイソシチジンと5-メチル-4-ピリミジノンヌクレオシドを導入したDNA三重鎖の塩基対形成能の評価2014

    • Author(s)
      山口 慧
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 塩基部修飾を導入した2'-0-[2-(N-メチルカルバモイル)エチル]修飾核酸の合成とその性質2013

    • Author(s)
      石井陽大、正木慶昭、劉天文、田胡信広、岡庭夏己、山田剛史、永田哲也、武田 伸一、金森功吏、大窪章寛、清尾康志、関根光雄
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島大学蔵本キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] T-A, C-G塩基対を含む配列で認識可能な新規三重鎖形成核酸の合成2013

    • Author(s)
      伊藤優、大窪章寛、山田健司、吉村貴一、金森功吏、正木慶昭、清尾康志、関根光雄
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島大学 蔵本キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] イミダゾリノン誘導体を導入した新規蛍光核酸の合成と蛍光特性2013

    • Author(s)
      金森功吏,高村亮宏,大窪章寛,関根光雄,清尾康志
    • Organizer
      第93春季年会プログラム
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 4-(2-グアニドエチル)ピリミジン誘導体を含む三重鎖形成核酸の合成と塩基識別能2013

    • Author(s)
      伊藤優,大窪章寛,山田健司,吉村貴一,金森功吏,正木慶昭,清尾康志,関根光雄
    • Organizer
      第93春季年会プログラム
    • Place of Presentation
      立命館大学びわこ・くさっキャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 四塩基対認識を指向した人工三重鎖形成核酸の合成と性質2013

    • Author(s)
      山田健司,大窪章寛,伊藤優,吉村貴一,金森功吏,正木慶昭,清尾康志,関根光雄
    • Organizer
      第93春季年会プログラム
    • Place of Presentation
      立命館大学びわこ・くさっキャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 新規 2′-O-修飾 RNA の開発とそのエキソン・スキッピング 活性2013

    • Author(s)
      関根光雄
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県・県民文化会館ひめぎんホール
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chemical synthesis of U1 snRNA derivatives2013

    • Author(s)
      小林春輝
    • Organizer
      第40回国際核酸化学シンポジウム
    • Place of Presentation
      神奈川大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] T-A,C-G塩基対を含む配列で認識可能な新規人工三重鎖形成核酸の開発2013

    • Author(s)
      伊藤 優
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 6-チオキサントシンおよび6-チオグアノシンを含む三重鎖形成核酸の合成と性質2013

    • Author(s)
      印出健志
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 塩基部修飾を導入した2’-O-[2-(N-メチルカルバモイル)エチル]修飾核酸の合成とその性質2013

    • Author(s)
      石井 陽大
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of fluorescent turn-on sensor for triplex formation and its application to nucleosides and oligonucleotides sensing2012

    • Author(s)
      Takashi Kanamori
    • Organizer
      The 39th International Symposium on Nucleic Acid Chemistry 2012
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] Synthesis and properties of new triplex forming oligonucleotides capable of recognizing TA and CG base pairs in parallel orientation2012

    • Author(s)
      Kenji Yamada
    • Organizer
      The 39th International Symposium on Nucleic Acid Chemistry 2012
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] A New Approach to the Synthesis of Modified Nucleosides Using Microflow Reactors2012

    • Author(s)
      Nobuhiro Tago
    • Organizer
      The 39th International Symposium on Nucleic Acid Chemistry 2012
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] Light-Control of Interaction Between Mismatch-Binding Protein MutS and the Photocaged Modified DNA2012

    • Author(s)
      Yurie Ohno
    • Organizer
      The 39th International Symposium on Nucleic Acid Chemistry 2012
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] Design and Synthesis of New Oligonucleotides Capable of Forming Triplexes Highly Selectively with DNA Duplexes without Binding to Single Stranded mRNAs2012

    • Author(s)
      関根光雄
    • Organizer
      2012 International Symposium on Advanced Biological Engineering (ISABE'2012)
    • Place of Presentation
      Guilin, Guangxi, China(桂林)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 4-O-(アミノエチル)ウリジン誘導体を含む新規三重鎖形成核酸の合成とその性質2012

    • Author(s)
      伊藤優, et al
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶應大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] DNA二重鎖および三重鎖に導入したデオキシシュードイソシチジンの塩基対形成能の評価2012

    • Author(s)
      山口慧, et al
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶應大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 6-チオプリンおよび4-チオピリミジン誘導体を含むオリゴヌクレオチドの合成とその三重鎖形成能2012

    • Author(s)
      印出健志, et al
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶應大学
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Synthesis and properties of new triplex forming oligonucleotides capable of recognizing TA and CG base pair in parallel orientation2012

    • Author(s)
      Kenji Yamada, Yu Ito, Kiichi Yoshimura, Takashi Kanamori, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine
    • Organizer
      The 39th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      Nagoya University(IGER)
    • Related Report
      2013 Final Research Report
  • [Presentation] チオカルボニル基を有する修飾塩基の連続導入による三重鎖形成能の向上2012

    • Author(s)
      山田健司,服部勇作,印出健志,金森功吏,大窪章寛,清尾康志,関根光雄
    • Organizer
      アンチセンス・遺伝子・デリバリーシンポジウム
    • Related Report
      2013 Final Research Report
  • [Presentation] Recent Studies on the Chemical Synthesis on Modified RNA OIigomers Directed towawd Genen Therapy2011

    • Author(s)
      Sekine, M
    • Organizer
      International Graduate Forum on Biotechnology, Bioengineering and Biomedical Science
    • Place of Presentation
      北京
    • Year and Date
      2011-08-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Synthesis and Properties of Oligonucleotides 2,2,7-Trimethyl- guanosine Cap Analogues2011

    • Author(s)
      Yokouchi, A.; Yamada, K.;Tsunoda, H.;Ohkubo, A.; Seio, K.;Sekine, M
    • Organizer
      2011 International Graduate Forum on Biotechnology, Bioengineering and Biomedical Science
    • Place of Presentation
      北京
    • Year and Date
      2011-08-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 新合成手法に基づく新規核酸医薬の開発2011

    • Author(s)
      関根光雄
    • Organizer
      千里ライフサイエンスセミナー
    • Place of Presentation
      千里
    • Year and Date
      2011-07-08
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 新規人工核酸の創成研究2011

    • Author(s)
      関根光雄
    • Organizer
      日本化学会関東支部主催群馬地区講演会
    • Place of Presentation
      群馬大学(招待講演)
    • Year and Date
      2011-07-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規人工核酸の創成研究2011

    • Author(s)
      関根光雄
    • Organizer
      日本化学会関東支部主催群馬地区講演会
    • Place of Presentation
      群馬大学
    • Year and Date
      2011-06-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Synthesis and Biological Properties of Synthesis and Biological Properties of 2'-O-Modified Oligoribonucleotide Derivatives2011

    • Author(s)
      Sekine, M.
    • Organizer
      XVth Symposium on Chemistry of Nucleic Acid Components SCNAC 2011
    • Place of Presentation
      Cechy, Ceska republika(招待講演)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] S-フェニルポスホロチオエート誘導体を利用した、固相上でのTMG-キャップ化反応の検討2011

    • Author(s)
      山田研,鈴木真,横内瑛,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶磨大学日吉キャンパス・矢上キャンパス
    • Year and Date
      2011-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Biological Properties of 2'-O-Modified Oligoribonucleotide Derivatives2011

    • Author(s)
      Sekine, M
    • Organizer
      XVth Symposium on Chemistry of Nucleic Acid Components SCNAC 2011
    • Place of Presentation
      Cechy, Ceska republika
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] TMGキャップアナロクを有するモルフォリノ核酸の合成2011

    • Author(s)
      山田研,横内瑛,鈴木真,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第21回アンチセンスシンポジウムおよび第11回遺伝子・デリバリー研究会シンポジウム
    • Place of Presentation
      大阪大学コンベンションセンター
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] 生体関連核酸誘導体の効率的合成法に関する研究2011

    • Author(s)
      関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶磨大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 4-0-(アミノエチル)ウリジン誘導体を含む新規三重鎖形成核酸の合成とその性質2011

    • Author(s)
      伊藤優,西野雄大,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶磨大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA二重鎖および三重鎖に導入したデオキシシュードイソシチジンの塩基対形成能の評価2011

    • Author(s)
      金森功吏,山口慧,角田浩佑,大窪章寛,関根光雄,清尾康志
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶慮大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 6-チオプリンおよび4一チオピリミジン誘導体を含むオリゴヌクレオチドの合成とその三重鎖形成能2011

    • Author(s)
      服部勇作,印出健志,山田健司,伊藤優,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶慮大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] アミノナフチリジン誘導体を含む新規三重鎖形成核酸の合成とその性質2011

    • Author(s)
      山田健司,服部勇作,伊藤優,印出健志,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶慮大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロフローリアクターを利用した修飾ヌクレオチドの新規合成法の開発2011

    • Author(s)
      田胡信広,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶慮大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] 3-メチルベンゾフランを導入したウリジン誘導体の合成と二重鎖構造及び三重鎖構造中での蛍光特性2011

    • Author(s)
      金森功吏,大関弘貴,徳川宗史,角田浩佑,大窪章寛,関根光雄,清尾康志
    • Organizer
      第92春季年会プログラム
    • Place of Presentation
      慶磨大学日吉キャンパス・矢上キャンパス
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Properties of Oligonucleotides Having 5'-Termina1 2,2,7-Trimethylguanosine Cap Analogues2011

    • Author(s)
      Yokouchi, A.;Yamada, K.;Tsunoda, H.; Ohkubo, A.;Seio, K.;Sekine, M
    • Organizer
      The 16th Annual Meeting of the RNA Society and The RNA Society of Japan 13th Annual Meeting
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Triplex Formation of RNAs Containing 8-Thioxoadenine and 2-Thiopyrimidine2011

    • Author(s)
      Ohkubo, A.;Ito, Y.; Nishino, Y.;Yokouchi, A.;Tsunoda, H.; Seio, K.;Sekine, M
    • Organizer
      The 16th Annual Meeting of the RNA Society and The RNA Society of Japan 13th Annual Meeting
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Final Research Report
  • [Presentation] 2'-O-カルハモイルウリシン誘導体を有する人工核酸の合成と性質2011

    • Author(s)
      徳川宗史,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第21回アンチセンスシンポジウムおよび第11回遺伝子・デリバリー研究会シンポジウム
    • Place of Presentation
      大阪大学コンベンションセンター
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Properties of Oligonucleotides 2,2,7-Trimethylguano-sine Cap Analogues2011

    • Author(s)
      Ohkubo, A.; Yokouchi, A.;Yamada, K.;Tsunoda, H.; Seio, K.;Sekine, M
    • Organizer
      6^<th> Cambridge Symposium on Nucleic Acids Chemistry and Biolo
    • Place of Presentation
      Queens'College, Cambridge, UK
    • Related Report
      2013 Final Research Report
  • [Presentation] Oligonucleotides 2,2,7-Trimethylguano- sine Cap Analogues2011

    • Author(s)
      Ohkubo, A.; Yokouchi, A.;Yamada, K.;Tsunoda, H.; Seio, K.;Sekine, M
    • Organizer
      7^<th> Annual Meeting of the Oligonucleotide Therapeutics Society
    • Place of Presentation
      The Royal Library, Copenhagen, Denmark
    • Related Report
      2013 Final Research Report
  • [Presentation] ホスホロアミダイト化合物を利用したオリゴヌクレオチド5'末端の新規アデニル化反応2011

    • Author(s)
      田胡信広,西野雄大,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場「エポカルつくば」
    • Related Report
      2013 Final Research Report
  • [Presentation] 2,2,7-トリメチルグアノシンキャップ構造およびそのアナログ構造を有するアンチセンス分子の合成と性質2011

    • Author(s)
      横内瑛,山田研,鈴木真,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場「エポカルつくば」
    • Related Report
      2013 Final Research Report
  • [Presentation] One-pot Sequential Formation of Pyro- and Triphosphate Bonds by Using O-N Phosphoryl Migration of Phosphoramidite Derivatives2011

    • Author(s)
      Tago, N.;Ohkubo, A.;Tsunoda, H.;Seio, K.;Sekine, M
    • Organizer
      The Seventh International Symposium on Integrated Syntheaia(ISIS-7)
    • Place of Presentation
      Seaside Hotel Maiko Vill, Kobe, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] An Effective Method for Phosphorodiamidate Bond Formation in the Synthesis of Morpholino Nucleic Acids2011

    • Author(s)
      Harakawa, T.; Ishii, A.; Tsunoda, H.; Ohkubo, A.;Seio, K.;Sekine, M
    • Organizer
      The 28th International Symposium on Nucleic Acid Chemistry(ISNAC2011)
    • Place of Presentation
      Clark Memorial Student Center, Hokkaido University, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Properties of Oligonucleotides Having 2,2,7-Trimethylguanosine Cap Analogs2011

    • Author(s)
      Yamada, K.;Yokouchi, A.;Suzuki, M.; Tsunoda, H.;Ohkubo, A.;Seio, K.;Nagata, T.;Aoki, Y.;Takeda, S.;Sekine, M
    • Organizer
      The 28^<th> International Symposium on Nucleic Acid Chemistry (ISNAC2011)
    • Place of Presentation
      Clark Memorial Student Center, Hokkaido University, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 1-アミノフタラジンを有する新規ペプチド核酸の合成2011

    • Author(s)
      佐藤祐太,金森功吏,角田浩佑,大窪章寛,関根光雄,清尾康志
    • Organizer
      第1回CSJ化学フェスタ2011世界化学年祈年大会
    • Place of Presentation
      早稲田大学大隈記念講堂・小野梓記念館
    • Related Report
      2013 Final Research Report
  • [Presentation] 徳川宗史,角田浩佑,大窪章寛,関根光雄,清尾康志2011

    • Author(s)
      2'-O-カルバモイルウリジン誘導体の化学合成と酵素耐性
    • Organizer
      第1回CSJ化学フェスタ2011世界化学年祈年大会
    • Place of Presentation
      早稲田大学大隈記念講堂・小野梓記念館
    • Related Report
      2013 Final Research Report
  • [Presentation] 2-チオピリミジンおよび8-チオキソプリンを含む修飾核酸の三重鎖形成能2011

    • Author(s)
      伊藤優,西野雄大,角田浩佑,大窪章寛,清尾康志,関根光雄
    • Organizer
      第1回CSJ化学フェスタ2011世界化学年祈年大会
    • Place of Presentation
      早稲田大学大隈記念講堂・小野梓記念館
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis and Properties of Modified Oligonucleotides Capable Triplex Formation with DNA Duplexes under Neutral Conditions2010

    • Author(s)
      M.Sekine
    • Organizer
      2010環太平洋国際化学会議
    • Place of Presentation
      Hawaii, Convention Center, USA
    • Year and Date
      2010-12-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2'-O-MCEオリゴヌクレオチドの合成と牲質2010

    • Author(s)
      山田剛史, et al.
    • Organizer
      第20回アンチセンスシンポジウム
    • Place of Presentation
      甲南大学ポートアイランドキャンパス
    • Year and Date
      2010-12-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Synthesis and Properties of 2'-O-[2-(N-methyl) carbamoyl]ethyl-RNAs2010

    • Author(s)
      T.Yamada, et al.
    • Organizer
      第37回国際核酸化学シンポジウム
    • Place of Presentation
      よこはま・はまぎんホール
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Synergetic effects of 2-thiolation and 5-propynylation of the uracil base on the stability of RNA duplexes2010

    • Author(s)
      R, Miyasaka, et al.
    • Organizer
      第37回国際核酸化学シンポジウム
    • Place of Presentation
      よこはま・はまぎんホール
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規核酸医薬の創成を指向した合成研究2010

    • Author(s)
      関根光雄
    • Organizer
      第54回日本薬学会関東支部大会
    • Place of Presentation
      東京薬科大学
    • Year and Date
      2010-10-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] RELATIONSHIP BETWEEN THERMAL STABILITY AND DEFORMABILITY OF MODIFIED RNA2010

    • Author(s)
      正木慶昭, et al.
    • Organizer
      XIX International Round Table on Nucleosides, Nucleotides and Nucleic Acids
    • Place of Presentation
      クロードベルナールリヨン第一大学
    • Year and Date
      2010-09-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2'水酸基修飾アンチセンスオリコヌクレオチトの合成と筋シストロフィーを対象としたエキソンスキッヒンク評価2010

    • Author(s)
      岡庭夏己, et al.
    • Organizer
      第12回 日本RNA学会年会
    • Place of Presentation
      一橋記念講堂:学術総合センター
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] New Triplex Forming Oligonucleotides Capable of Binding to DNA Duplexes under Neutral Conditions2010

    • Author(s)
      M.Sekine
    • Organizer
      Heidelberg University-Tokyo Institute of Technology Joint Symposium on
    • Place of Presentation
      Wroclaw University, Poland
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Triplex Forming Oligonucleotides Containing Thiolated Nucleobases2010

    • Author(s)
      M.Sekine
    • Organizer
      ICPC2010-Symposium on Biophosphates
    • Place of Presentation
      Wroclaw Lniversity, Poland
    • Year and Date
      2010-07-13
    • Related Report
      2010 Annual Research Report
  • [Book] Synthesis and Exon-Skipping Assay of Chemically Modified RNAs, in RNA Technologies: DNA and RNA Chemical Biology in Science and Medicine, ed. V. A. Erdmann, W. T. Markiewicz, and J. Barciszewski. Chaptor 192014

    • Author(s)
      Y. Masaki, T. Yamada, H. Saneyoshi, A. Ohkubo, K. Seio, and M. Sekine
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.skn.bio.titech.ac.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2018-03-28  

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