| Budget Amount *help |
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Research Abstract |
In this study, we suceeded in synthesizing the full structure of U1snRNA, which was very difficult to synthesize, by using sequential condensations in the solid-phase. This 5'-terminal 2,2,7-trimethylguanosine cap structure has a function capable of passing through the nuclear membrane from the cytoplasm. The full structure of U1snRNA was constructed for the first time in the world by ligation with an RNA transcript. We also studied the synthesis of 2'-O-MCE-RNA phosphorothioates which have proved to be promising as drugs for treatment of muscular dystrophy. They could be obtained in good yields by using the optimized conditions. A uridine derivative required for this molecule was successfully synthesized by a microflow system.
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