| Budget Amount *help |
¥59,150,000 (Direct Cost: ¥45,500,000、Indirect Cost: ¥13,650,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2010: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2009: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
|
|---|
| Research Abstract |
The 5' terminal of Xist RNA, a nuclear retained non-coding RNA which inactivates one of the pair of X chromosomes, was found to be characteristically modified unlike ordinary mRNAs. Also, the conserved element in 3' terminal of Malat1 RNA, the component of a functional nuclear compartment "nuclear speckles", was found to be methylated at an adenosine site. Identification of a gene diminishing the methylated adenosine revealed the most plausible structure and modifying enzymes of the methyladenosine. We developed methylthio-modification measurement method by using small-quantity clinical samples. Taken together, we contributed the findings of RNA structural alterations, including determination of a novel chemical structure, and their modifying factors.
|
