| Budget Amount *help |
¥213,720,000 (Direct Cost: ¥164,400,000、Indirect Cost: ¥49,320,000)
Fiscal Year 2013: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2012: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2011: ¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
Fiscal Year 2010: ¥30,420,000 (Direct Cost: ¥23,400,000、Indirect Cost: ¥7,020,000)
Fiscal Year 2009: ¥71,760,000 (Direct Cost: ¥55,200,000、Indirect Cost: ¥16,560,000)
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| Outline of Final Research Achievements |
In this study, we were engaged to develop the methods for in-cell NMR of proteins and optically detected magnetic resonance (ODMR), aiming to create experimental techniques to provide structural and dynamics information of protein complexes formed transiently in cells or at cell membranes. For in-cell NMR, we used the signal transduction factor in immunological system FKBP12 and a chaperon protein for fatty acids, FABP4. We could measure in-cell 1H-15N correlation and 19Ｆ－ＮＭＲ spectra of them, respectively. For ODMR, we have built methods to selectively detect fluorescence of diamond particles and to determine the directions of particles.
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