| Budget Amount *help |
¥109,850,000 (Direct Cost: ¥84,500,000、Indirect Cost: ¥25,350,000)
Fiscal Year 2014: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2010: ¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
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| Outline of Final Research Achievements |
In order to advance visualization analysis by “in vivo” two-photon microscopy, we combined the microscopy with cutting-edge technologies of laser optics and fluorescent probes novel visualization as well as developed a new analysis technique for estimating optical properties in living bodies. We improved the penetration depth and the spatial resolution in two-photon microscopy, and successfully demonstrated “intact” visualization of hippocampal dentate gyrus in living mouse brains. Furthermore, we created a method for optical manipulation and stimulation, and successfully archived laser ablation of neural fibers within deeper layers in living mouse brains.
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