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Molecular analysis of signaling mechanism of immune cell surface receptors

Planned Research

Project AreaStructural basis of cell-signalling complexes mediating signal perception, transduction and responses
Project/Area Number 22121007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionHokkaido University

Principal Investigator

MAENAKA KATSUMI  北海道大学, 薬学研究科(研究院), 教授 (10322752)

Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥109,720,000 (Direct Cost: ¥84,400,000、Indirect Cost: ¥25,320,000)
Fiscal Year 2014: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2012: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2011: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2010: ¥27,690,000 (Direct Cost: ¥21,300,000、Indirect Cost: ¥6,390,000)
Keywords蛋白質 / 免疫 / 感染症 / X線結晶構造解析 / 受容体 / 創薬科学 / 免疫学 / ウイルス / 生体分子 / 薬学 / ペア型レセプター / 細胞表面受容体 / 蛋白質間相互作用 / 表面プラズモン共鳴 / NMR解析 / 免疫制御
Outline of Final Research Achievements

To understand medically important immune response and infection route, it is essential to structure biologicallyvisualize the mutual recognition mechanism in atomic level of immune cell surface receptor as the defense forefront toward cancer cells and pathogens. In this study, as important signal control complexes in immune diseases and infectious diseases, we focused on HVEM signal control complex of co-signaling molecule CD160 on T cells, the complex of NKR-P1 / CD161 receptor expressed in human Th17 cells with ligand LLT1, measles virus surface protein invasion complexes, glycolipid recognition immunoreceptor Mincle and MCL. Structural features extracted from the three-dimensional structures and physicochemical analyses of these complexes promoted advance field "structural molecular medicine".

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (27 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Invited: 4 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Crystal structure of extracellular domain of human lectin-like transcript 1 (LLT1), the ligand for natural killer receptor-P1A.2015

    • Author(s)
      Kita S, Matsubara H, Kasai Y, Tamaoki T, Okabe Y, Fukuhara H, Kamishikiryo J, Krayukhina E, Uchiyama S, Ose T, Kuroki K, Maenaka K.
    • Journal Title

      Eur. J. Immunol.

      Volume: 印刷中 Issue: 6 Pages: 1605-1613

    • DOI

      10.1002/eji.201545509

    • NAID

      120005906772

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The structural basis for receptor recognition of human interleukin-182014

    • Author(s)
      Naotaka Tsutsumi, Takeshi Kimura, Kyohei Arita, Mariko Ariyoshi, Hidenori Ohnishi, Takahiro Yamamoto, Xiaobing Zuo, Katsumi Maenaka, Enoch Y. Park, Naomi Kondo, Masahiro Shirakawa, Hidehito Tochio and Zenichiro Kato
    • Journal Title

      Nat. Commun.

      Volume: 5 5340 Issue: 1 Pages: 1-13

    • DOI

      10.1038/ncomms6340

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL.2013

    • Author(s)
      Furukawa A, Ynmasaki S, Maenaka, K, et al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA.

      Volume: 110 Issue: 43 Pages: 17438-43

    • DOI

      10.1073/pnas.1312649110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibiting HLA-B27 homodimer-driven immune cell inflammation in spondylarthritis.2012

    • Author(s)
      11. Payeli SK, Kollnberger S, Osiris Marroquin B, Thiel M, McHugh K, Giles J, Shaw J, Kleber S, Ridley A, Wong-Baeza I, Keidel S, Kuroki K, Maenaka K, Wadle A, Renner C, Bowness P.
    • Journal Title

      Arthritis Rheum.

      Volume: 64 Issue: 10 Pages: 3139-3149

    • DOI

      10.1002/art.34538

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HLA-B27 homodimers and free H chains are stronger ligands for leukocyte Ig-like receptor B2 than classical HLA class I.2012

    • Author(s)
      Giles Jら
    • Journal Title

      J Immunol.

      Volume: 188 Issue: 12 Pages: 6184-6193

    • DOI

      10.4049/jimmunol.1102711

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair2012

    • Author(s)
      Yoshida, S.
    • Journal Title

      J.Immunol.

      Volume: 188 Issue: 8 Pages: 3972-3979

    • DOI

      10.4049/jimmunol.1102886

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo.2012

    • Author(s)
      Arai R
    • Journal Title

      European journal of cancer(Oxford,England:1990)

      Volume: 48 Issue: 15 Pages: 2417-2430

    • DOI

      10.1016/j.ejca.2011.12.028

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT2011

    • Author(s)
      Rieko Kojima, et al
    • Journal Title

      Journal of Molecular Biology

      Volume: 413(4) Issue: 4 Pages: 762-772

    • DOI

      10.1016/j.jmb.2011.09.018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization Strategy for the Glycoprotein-receptor Complex Between Measles Virus Hemagglutinin and its Cellular Receptor SLAM2011

    • Author(s)
      Hashiguchi, ら
    • Journal Title

      Protein Pept.Lett.

      Volume: 19 Pages: 468-473

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential but competitive binding of Nogo and MHCI to PIR-B provides an inhibition of cells2011

    • Author(s)
      Matsushita H, et al
    • Journal Title

      J.Biol.Chem.

      Volume: 286(29) Issue: 29 Pages: 25739-25747

    • DOI

      10.1074/jbc.m110.157859

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis for LLT1 recognition by human CD161 (NKRP1A/KLRB1)2011

    • Author(s)
      Kamishikiryo, ら
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 27 Pages: 23823-23830

    • DOI

      10.1074/jbc.m110.214254

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A fluorescent single domain antibody against plumbagin expressed in silkworm larvae for fluorescence-linked immunosorbent assay (FLISA)2011

    • Author(s)
      Sakamoto, ら
    • Journal Title

      Analyst.

      Volume: 136 Issue: 10 Pages: 2056-2063

    • DOI

      10.1039/c1an15027h

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction, expression, and characterization of a single-chain variable fragment antibody against 2,4-dichlorophenoxyacetic acid in the hemolymph of silkworm larvae2011

    • Author(s)
      Sakamoto, ら
    • Journal Title

      Appl Biochem Biotechnol.

      Volume: 164 Issue: 6 Pages: 715-728

    • DOI

      10.1007/s12010-011-9168-4

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the measles virus hemagglutinin bound to its cellular receptor SLAM.2011

    • Author(s)
      Hashiguchi, ら
    • Journal Title

      Nat Struct Mol Biol.

      Volume: 18 Pages: 135-141

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction, Expression, and Characterization of a Single-Chain Variable Fragment Antibody Against 2, 4-Dichlorophenoxyacetic Acid in the Hemolymph of Silkworm Larvae.2011

    • Author(s)
      Sakamot, ら
    • Journal Title

      Appl Biochem Biotechnol.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in enzyme-linked immunosorbent assay for quality control of total ginsenosides.2010

    • Author(s)
      Sakamot, ら
    • Journal Title

      J Biochem.

      Volume: 148 Pages: 335-340

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 免疫系受容体Mincle とMCL の糖脂質認識の分子基盤2015

    • Author(s)
      前仲勝実
    • Organizer
      日本薬学会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 糖脂質・糖ペプチドを認識する免疫系受容体の構造生物学的研究2014

    • Author(s)
      前仲勝実
    • Organizer
      日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] パラミクソウイルス侵入機構と阻害剤開発2013

    • Author(s)
      前仲勝実
    • Organizer
      第133回日本薬学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Structural basis of glycolipids recognition mechanisms by C-type lectin like receptor, Mincle.2013

    • Author(s)
      Furukawa A, Kamishikiryo J, Mori D, Toyonaga K, Okabe Y, Toji A, Kanda R, Miyake Y, Ose T, Yamasaki S, Maenaka K.
    • Organizer
      International Conference on Structural Genomics 2013 -Structural Life Science
    • Place of Presentation
      京王プラザホテル札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 表面タンパク質の不安定な複合体の分子解析2011

    • Author(s)
      前仲勝実
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪)(招待講演)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 麻疹ウイルスの細胞侵入機構2010

    • Author(s)
      前仲勝実
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 自己免疫疾患に関わるHLAクラスI分子による免疫制御の分子基盤

    • Author(s)
      前仲勝実
    • Organizer
      日本脊椎関節炎学会第23回学術集会
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] タンパク質結晶の最前線 (試料作製技術の章を担当)2013

    • Author(s)
      喜多等
    • Total Pages
      500
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Annual Research Report
  • [Book] 薬系免疫学 第2版2012

    • Author(s)
      前仲勝実
    • Total Pages
      240
    • Publisher
      南江堂
    • Related Report
      2012 Annual Research Report
  • [Remarks] 構造細胞生物学ホームページ

    • URL

      http://convallaria.pharm.hokudai.ac.jp/scb/

    • Related Report
      2014 Annual Research Report
  • [Remarks] ワクチンの効果を高める免疫賦活剤に対する免疫細胞受容体の構造解析(薬学研究院 教授 前仲勝実)

    • URL

      http://www.hokudai.ac.jp/news/131008_pr_pharm.pdf

    • Related Report
      2013 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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