Analysis for the functional significance of area and layer selective genes

• Project Area: Neural Diversity and Neocortical Organization
• Project/Area Number: 22123009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Institute of Physical and Chemical Research (2014-2015); National Institute for Basic Biology (2010-2013)
• Principal Investigator: Yamamori Tetsuo 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (80260206)
• Co-Investigator(Kenkyū-buntansha): 川口 泰雄 生理学研究所, 大脳皮質研究部門, 教授 (40169694) ; 窪田 芳之 生理学研究所, 大脳皮質機能研究系, 准教授 (90192567) ; 佐々木 哲也 国立精神・神経医療研究センター, 微細構造研究部, 研究員 (10634066) ; 木津川 尚史 大阪大学, 生命機能研究科, 准教授 (10311193)
• Co-Investigator(Renkei-kenkyūsha): 坂野 仁 福井大学, 医学部, 特命教授 (90262154)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥175,630,000 (Direct Cost: ¥135,100,000、Indirect Cost: ¥40,530,000); Fiscal Year 2014: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000); Fiscal Year 2013: ¥35,230,000 (Direct Cost: ¥27,100,000、Indirect Cost: ¥8,130,000); Fiscal Year 2012: ¥32,110,000 (Direct Cost: ¥24,700,000、Indirect Cost: ¥7,410,000); Fiscal Year 2011: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000); Fiscal Year 2010: ¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
• Keywords: 大脳皮質 / 層 / 領野 / ウイルスベクター / ２重感染 / 霊長類 / 神経科学 / 大脳皮質領野 / 遺伝子発現 / 視覚野 / 連合野

Outline of Final Research Achievements

We identified the two group of genes that are selectively expressed in the primate neocortical areas using large scale analysis: One group of the genes is specifically expressed in the association areas and the other is specifically expressed in the primary visual area. In this study, we found the following results during the term. (1) We examined the promotor region of the association area selective genes and found highly methylated while that of the primary visual area is little methylated. We then found that MBD4 regulated the association selectively expressed genes (PNMA5 and RBP4). (2) We confirmed the existence of the ocular dominance column in the primary visual cortex in marmosets using immediate early gene expression. (3) We developed the virus vector systems so that we are able to apply the systems to the primate neocortex.

Research Products

• [Journal Article] Long-term calcium imaging of neuronal population with sub cellular resolution in adult non-human primates. (2015)
  • Author(s): Sadakane, O., Masamizu, Y., Watakabe, A., Terada, S., Ohtsuka, M., Takaji, M., Mizukami, H,Ozawa, K., Kawasaki, K., Matsuzaki, M., Yamamori, T.
  • Journal Title: Cell Reports Volume: 13 Pages: 1989-1999
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vitro two-photon imaging of dendritic spines in marmoset neocortex. (2015)
  • Author(s): Sadakane, O., Watakabe, A., Ohtsuka, M., Takaji, M., Sasaki, T., Kasai, M., Isa, T., Kato, G., Nabekura, J., Mizukami, H., Ozawa, K., Kawasaki, H., Yamamori, T
  • Journal Title: eNeuro Volume: 2 Pages: 4-4
  • Peer Reviewed / Open Access
• [Journal Article] Expression pattern of immediate early genes in the cerebellum of D1R KO, D2RKO and wild type mice under vestibular-controlled activity. (2015)
  • Author(s): Nakamura, T., Sato, A., Kitsukawa, T., Sasaoka, T., Yamamori, T.
  • Journal Title: Front Cell Dev. Biol. Volume: 3 Pages: 38-38
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparative analysis of adeno-associated viral vector seotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex (2015)
  • Author(s): Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, T., Yamamori, T.
  • Journal Title: Neuroscience Research Volume: 93 Pages: 144-157
  • Peer Reviewed / Open Access
• [Journal Article] Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset (2013)
  • Author(s): Yuki Nakagami, Akiya Watakabe and Tetsuo Yamamori
  • Journal Title: Frontiers in Neural Circuits Volume: 7
  • DOI: 10.3389/fncir.2013.00043
  • Peer Reviewed
• [Journal Article] DNA Methylation and Methyl-Binding Proteins Control Differential Gene Expression in Distinct Cortical Areas of Macaque Monkey. (2013)
  • Author(s): Katsusuke Hata, Hiroaki Mizukami, Osamu Sadakane, Akiya Watakabe, Masanari Ohtsuka, Masafumi Takaji, Masaharu Kinoshita, Tadashi Isa, Keiya Ozawa, and Tetsuo Yamamori
  • Journal Title: The Journal of Neuroscience Volume: 33 Issue: 50 Pages: 19704-19714
  • DOI: 10.1523/jneurosci.2355-13.2013
  • Peer Reviewed
• [Journal Article] Diversity of Retinal Ganglion Cells Identified by Transient GFP Transfection in Organotypic Tissue Culture of Adult Marmoset Monkey Retina (2013)
  • Author(s): Satoru Moritoh, Yusuke Komatsu, Tetsuo Yamamori, Amane Koizumi
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e54667-e54667
  • DOI: 10.1371/journal.pone.0054667
  • Peer Reviewed
• [Journal Article] Behavioral abnormalities observed in Zfhx2-deficient mice (2012)
  • Author(s): Yuriko Komine, Keizo Takao, Tsuyoshi Miyakawa, Tetsuo Yamamori
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: 12-12
  • DOI: 10.1371/journal.pone.0053114
  • Peer Reviewed
• [Journal Article] Visualization of the cortical projection neurons by retrograde TET-OFF lentiviral vector. (2012)
  • Author(s): 渡我部昭哉, 他
  • Journal Title: PLos One Volume: 7 Issue: 10 Pages: e46157-e46157
  • DOI: 10.1371/journal.pone.0046157
  • Peer Reviewed
• [Journal Article] Genetic dissection of the circuit for hand dexterity in primates (2012)
  • Author(s): Masaharu Kinoshita, Ryosuke Matsui, Shigeki Kato, Taku Hasegawa, Hironori Kasahara, Kaoru Isa, Akiya Watakabe, Tetsuo Yamamori, Yukio Nishimura, Bror Alstermark, Dai Watanabe, Kazuto Kobayashi & Tadashi Isa
  • Journal Title: Nature Volume: 487 Issue: 7406 Pages: 235-238
  • DOI: 10.1038/nature11206
  • Peer Reviewed
• [Journal Article] Area-Specific Substratification of Deep Layer Neurons in the Rat Cortex (2012)
  • Author(s): Akiya Watakabe, Junya Hirokawa, Noritaka Ichinohe, Sonoko Ohsawa, Takeshi Kaneko, Kathleen S. Rockland, and Tetsuo Yamamori
  • Journal Title: The Journal of Comparative Neurology Volume: 520(16) Issue: 16 Pages: 3553-3573
  • DOI: 10.1002/cne.23160
  • Peer Reviewed
• [Journal Article] 霊長類の大脳皮質視覚野と連合野で顕著に発現する遺伝子の機能的意義 (2012)
  • Author(s): 山森 哲雄
  • Journal Title: 生体の科学 Volume: 63 Pages: 592-598
  • NAID: 40019532174
• [Journal Article] Differential expression patterns of striate cortex-enriched genes among Old World, New World, and prosimian primates (2012)
  • Author(s): Toru Takahata, Rammohan Shukla, Tetsuo Yamamori and Jon H. Kaas
  • Journal Title: Cerebral Cortex Volume: 22(10) Issue: 10 Pages: 2313-2321
  • DOI: 10.1093/cercor/bhr308
  • Peer Reviewed
• [Journal Article] Multisensory information facilitates reaction speed by enlarging activity difference between superior colliculus hemispheres in rats (2011)
  • Author(s): Junya Hirokawa, Osamu Sadakane, Shuzo Sakata, Miquel Bosch, Yoshio Sakurai, Tetsuo Yamamori
  • Journal Title: PLoS ONE Volume: 6(9) Issue: 9 Pages: 1-13
  • DOI: 10.1371/journal.pone.0025283
  • Peer Reviewed
• [Journal Article] Roberto Sprea fico, and Rita Garbelli (2011)
  • Author(s): Laura Rossini, Ramona F. Moroni, Laura Tassi, Akiya Watakabe, Tetsuo Yamamori
  • Journal Title: Altered layer-specific gene expression in cortical samples from patients with temporal lobe epilepsy Volume: 52(10) Issue: 10 Pages: 1928-1937
  • DOI: 10.1111/j.1528-1167.2011.03246.x
  • Peer Reviewed
• [Journal Article] Selective gene expression in regions of primate neocortex: Implications for cortical specialization (2011)
  • Author(s): Tetsuo Yamamori
  • Journal Title: Progress in Neurobiology Volume: 94(3) Issue: 3 Pages: 201-222
  • DOI: 10.1016/j.pneurobio.2011.04.008
  • Peer Reviewed
• [Journal Article] A novel instrumented multipeg running wheel system, Step-Wheel, for monitoring and controlling complex sequential stepping in mice (2011)
  • Author(s): Kitsukawa T
  • Journal Title: J Neurophys iol Volume: 106 Issue: 1 Pages: 479-487
  • DOI: 10.1152/jn.00139.2011
  • Peer Reviewed
• [Journal Article] Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system (2010)
  • Author(s): Watakabe A
  • Journal Title: Methods Volume: 52 Pages: 367-374
  • Peer Reviewed
• [Journal Article] Differential expression patterns of OCC1-related, extracellular matrix proteins in the lateral geniculate nucleus of macaque monkeys (2010)
  • Author(s): Takahata T
  • Journal Title: Journal of Chemical Neuroanatomy Volume: 40 Pages: 112-122
  • Peer Reviewed
• [Journal Article] Serotonin modulates fast-spiking interneuron and synchronous activity in the rat prefrontal cortex through 5-HT1A and 5-HT2A receptors (2010)
  • Author(s): Puig MV
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 2211-2222
  • Peer Reviewed
• [Journal Article] Prefrontal-enriched SLIT1 expression in Old World monkey cortex established during the postnatal development (2010)
  • Author(s): Sasaki T
  • Journal Title: Cerebral Cortex Volume: 20 Pages: 2496-2510
  • Peer Reviewed
• [Presentation] Two-photon calcium imaging using genetically-encoded calcium indicator in primate neocortex (2015)
  • Author(s): Sadakane O, Masamizu Y, Watakabe A, Terada S, Ohtsuka M, Takaji M, Mizukami H, Ozawa K, Kawasaki H, Matsuzaki M, Yamamori T
  • Organizer: Neuroscience 2015
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] The function and the regulation of the genes selectively expressed in the primate neocortex. (2015)
  • Author(s): Yamamori, T
  • Organizer: 中国神経科学学会
  • Place of Presentation: Wuzhen , China
  • Year and Date: 2015-09-23
• [Presentation] マーモセット大脳皮質におけるGCaMPを用いた2光子カルシウムイメージング (2015)
  • Author(s): 定金 理、正水 芳人、渡我部 昭哉、寺田 晋一郎、大塚 正成、高司 雅史、水上 浩明、小澤 敬也、河崎 洋志、松崎 政紀、山森 哲雄
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（神戸市）
  • Year and Date: 2015-07-28
• [Presentation] 発生過程のマウス脳に対する各AAVセロタイプ（1,2,8,9）およびレンチウィルスの感染特性の比較 (2013)
  • Author(s): 大塚 正成、水上 浩明、小澤 敬也、渡我部 昭哉、山森 哲雄
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 霊長類脳における遺伝子操作法の開発 (2013)
  • Author(s): 山森 哲雄
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] TET2重感染法(TEDI)を使った大脳皮質投射ニューロンサブタイプの同時標識 (2013)
  • Author(s): 渡我部 昭哉、加藤 成樹、小林 和人、水上 浩明、小澤 敬也、山森 哲雄
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] マーモセット大脳皮質におけるスパインの生体内可視化 (2013)
  • Author(s): 定金 理、渡我部 昭哉、大塚 正成、高司 雅史、佐々木 哲也、笠井 昌俊、伊佐 正、鍋倉 淳一、水上 浩明、小澤 敬也、河崎 洋志、山森 哲雄
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Gene manipulation in the marmoset brain (2013)
  • Author(s): Tetsuo Yamamori
  • Organizer: 第3回日本マーモセット研究会大会
  • Place of Presentation: 九州大学医学部「百年講堂」（福岡県）
• [Presentation] The ocular dominance column and gene expression upon light stimulation in the marmoset primary visual cortex. (2013)
  • Author(s): 山森 哲雄
  • Organizer: 国際シンポジウム「感覚受容と神経回路」
  • Place of Presentation: 東京大学（東京都）
• [Presentation] Dynamic changes of gene expression in the primary visual cortex revealed by monocular activation in adult marmosets. (2013)
  • Author(s): 仲神 友貴，渡我部 昭哉，山森 哲雄
  • Organizer: 国際シンポジウム「感覚受容と神経回路」
  • Place of Presentation: 東京大学（東京都）
• [Presentation] The ocular dominance column and gene expression upon light stimulation in the marmoset primary visual cortex. (2013)
  • Author(s): 山森 哲雄
  • Organizer: 第2回 日本マーモセット研究会 大会
  • Place of Presentation: 慶応義塾大学（東京都）
• [Presentation] マーモセット一次視覚野において光刺激で惹起される遺伝子群の経時的発現解析 (2012)
  • Author(s): 仲神 友貴，渡我部 昭哉，山森 哲雄
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Visualization of the cortical projection neuron subtypes by TET-double infection method (TEDI). (2012)
  • Author(s): A. WATAKABE, S. KATO, K. KOBAYASHI, H. MIZUKAMI, K. OZAWA, T. YAMAMORI
  • Organizer: Neuroscience 2012
  • Place of Presentation: Ernest N. Morial Convention Center (USA)
• [Presentation] Light induction causes dynamic alteration in the expression of activity-dependent genes in the marmoset primary visual cortex. (2012)
  • Author(s): Y. NAKAGAMI, A. WATAKABE, T. YAMAMORI
  • Organizer: Neuroscience 2012
  • Place of Presentation: Ernest N. Morial Convention Center (USA)
• [Presentation] Lentivirus-mediated gene delivery of cortical area-specific promoter-eGFP to the marmoset monkey cortical slice culture (2011)
  • Author(s): Katsusuke Hata
  • Organizer: Neuroscience 2011
  • Place of Presentation: Walter E.Washington Convention Center (USA)
  • Year and Date: 2011-11-15
• [Presentation] Comparison of layer 4 neurons in the primary visual cortex and the barrel cortex (2011)
  • Author(s): Osamu Sadakane
  • Organizer: Neuroscience 2011
  • Place of Presentation: Walter E.Washington Convention Center (USA)
  • Year and Date: 2011-11-13
• [Presentation] Evolutionary changes in V1-selective gene expression in primate species (2011)
  • Author(s): Toru Takahata
  • Organizer: Neuroscience 2011
  • Place of Presentation: Walter E.Washington Convention Center (USA)
  • Year and Date: 2011-11-13
• [Presentation] TET2重感染法(TEDI)による皮質視床投射ニューロン形態の可視化 (2011)
  • Author(s): 渡我部昭哉
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] マウス初期感覚野入力層ニューロンの比較 (2011)
  • Author(s): 定金理
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] 霊長類大脳新皮質におけるSLIT1mRNA発現の発生変化 (2011)
  • Author(s): 佐々木哲也
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-17
• [Presentation] Neuronal oscillation and sensory-motor integration in superior colliculus (2010)
  • Author(s): J.HIROKAWA
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2010-11-17
• [Presentation] Tet-off lentiviral vectors drive high-level transgene expression in marmoset brains (2010)
  • Author(s): A.WATAKABE
  • Organizer: Neuroscience 2010
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2010-11-16
• [Presentation] 霊長類前頭前皮質に強いSLIT1の発現パターンは生後発生で確立する (2010)
  • Author(s): 佐々木哲也
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-03
• [Presentation] TET-OFFレンチウイルスベクターによるマーモセット脳への遺伝子導入 (2010)
  • Author(s): 渡我部昭哉
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-02
• [Remarks] 山森研究室 - 基礎生物学研究所
  • URL: http://www.nibb.ac.jp/divspe1/
• [Remarks] 山森研究室 - 基礎生物学研究所
  • URL: http://www.nibb.ac.jp/divspe1/
• [Remarks]
  • URL: http://www.nibb.ac.jp/~divspe1/
• [Remarks]
  • URL: http://www.nibb.ac.jp/~divspe1/