| Co-Investigator(Kenkyū-buntansha) |
OTA Masao 信州大学, 医学部, 准教授 (50115333)
HOSOMICHI Kazuyoshi 金沢大学, 医学系, 准教授 (50420948)
IMANISHI Tadashi 東海大学, 医学部, 教授 (80270461)
OHTSHUKA Masato 東海大学, 医学部, 准教授 (90372945)
猪子 英俊 東海大学, 医学部, 教授 (10101932)
木村 穣 東海大学, 医学部, 教授 (10146706)
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| Budget Amount *help |
¥260,130,000 (Direct Cost: ¥200,100,000、Indirect Cost: ¥60,030,000)
Fiscal Year 2014: ¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2013: ¥50,960,000 (Direct Cost: ¥39,200,000、Indirect Cost: ¥11,760,000)
Fiscal Year 2012: ¥53,430,000 (Direct Cost: ¥41,100,000、Indirect Cost: ¥12,330,000)
Fiscal Year 2011: ¥52,910,000 (Direct Cost: ¥40,700,000、Indirect Cost: ¥12,210,000)
Fiscal Year 2010: ¥54,340,000 (Direct Cost: ¥41,800,000、Indirect Cost: ¥12,540,000)
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| Outline of Final Research Achievements |
Efficient resequencing of the entire 3.8 Mb HLA region was performed by the long-range PCR and sequence capture methods followed by next generation sequencing. The HLA sequences derived from seven HLA haplotypes were characterized among the different HLA haplotypes and also among the same HLA haplotypes. A field 4 level super high resolution single molecule - sequence based typing (SS-SBT) method was developed for HLA 11 loci using next generation sequencers. Polymorphism analysis on some HLA associated diseases such as Narcolepsy, AIP and PBD were performed at the field 4 level without phase ambiguity by the SS-SBT method. An HLA integrated database (HLA Information Resource; HLA-IR) was constructed as a public database. They will also contribute to the foundation for a refined knowledge of variation and its haplotype structure throughout the extremely polymorphic and gene-rich HLA maps, providing important information on the process of molecular evolution in the HLA region.
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