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Evaluation of cellular functions by imaging cellular nucleic acids

Planned Research

Project AreaMolecular Science for Nanomedicine
Project/Area Number 23107007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTokyo Institute of Technology (2013-2015)
Kyushu University (2011-2012)

Principal Investigator

Maruyama Atsushi  東京工業大学, 生命理工学研究科, 教授 (40190566)

Co-Investigator(Kenkyū-buntansha) SHIMADA NAOHIKO  東京工業大学, 大学院生命理工学研究科, 助教 (10423972)
狩野 有宏  九州大学, 先導物質化学研究所, 准教授 (30403950)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥99,190,000 (Direct Cost: ¥76,300,000、Indirect Cost: ¥22,890,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2012: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2011: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Keywordsカチオン性高分子 / DNA / 核酸酵素 / ペプチド / 脂質膜 / グラフト共重合体 / 高分子電解質複合体 / 核酸検出 / グラフト共重合体」 / DNA / 酵素 / ターンオーバー / カチオン性共重合体 / リポソーム / 生体膜 / ベシクル-シート転移 / 薬物送達 / デオキシリボザイム / 遺伝子解析 / カチオン性くし型共重合体 / ハイブリダイゼーション / 核酸シャペロン / 細胞内デリバリー / DDS / DNA鎖交換 / くし型共重合体 / 両親媒性ペプチド
Outline of Final Research Achievements

In this study, we showed that the cationic copolymer also enhances activity of an MNAzyme-type DNA sensor derived from the 10-23 DNAzyme and increased MNAzyme sensitivity. Furthermore, the copolymer enabled us to shorten the substrate-binding arms of the MNAzyme, decreasing the optimum temperature of the MNA assay from 50 °C to physiological temperature.
It was also demonstrated that the copolymer enhanced function of membrane disrupting peptides by facilitating their folding. Effective cytosolic delivery of macromolecular substances would be possible by using peptide/copolymer complex.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 2012 2011 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results,  Open Access: 5 results,  Acknowledgement Compliant: 8 results) Presentation (24 results) (of which Int'l Joint Research: 7 results,  Invited: 18 results)

  • [Journal Article] Preparation of upper critical solution temperature (UCST) responsive diblock copolymers bearing pendant ureido groups and their micelle formation behavior in water2016

    • Author(s)
      A. Fujihara, N. Shimada, A. Maruyama, K. Ishihara, S. Yusa
    • Journal Title

      Soft Matter

      Volume: - Issue: 26 Pages: 5204-5213

    • DOI

      10.1039/c5sm00499c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inter-polyelectrolyte nano-assembly induces folding and activation of functional peptides2015

    • Author(s)
      N. Shimada, H. Kinoshita, S, Tokunaga, T. Umegae, N. Kume, W. Sakamoto, A. Maruyama
    • Journal Title

      J. Controlled Rel.

      Volume: 218 Pages: 45-52

    • DOI

      10.1016/j.jconrel.2015.10.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA Microenvironment Monitored by Controlling Redox Blinking2015

    • Author(s)
      K. Kawai, K. Higashiguchi, A. Maruyama, and T. Majima
    • Journal Title

      ChemPhysChem

      Volume: 16 Issue: 17 Pages: 3590-3594

    • DOI

      10.1002/cphc.201500793

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] MNAzyme-catalysed nucleic acid detection enhanced by a cationic copolymer2015

    • Author(s)
      J. Gao, N. Shimada, A. Maruyama
    • Journal Title

      Biomater. Sci.

      Volume: 3 Issue: 5 Pages: 716-720

    • DOI

      10.1039/c4bm00449c

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Enhancement of deoxyribozyme activity by cationic copolymers2015

    • Author(s)
      J. Gao, N. Shimada, A. Maruyama
    • Journal Title

      Biomater. Sci

      Volume: 3 Issue: 2 Pages: 308-316

    • DOI

      10.1039/c4bm00256c

    • NAID

      120006582450

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Smart hydrogels exhibiting UCST-type volume changes under physiologically relevant conditions2014

    • Author(s)
      N. Shimada, S. Kidoaki, A. Maruyama
    • Journal Title

      RSC Advances

      Volume: 4 Issue: 94 Pages: 1480-1485

    • DOI

      10.1039/c4ra10612a

    • NAID

      120006582365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Drastic stabilization of parallel DNA hybridizations by a polylysine omb-type copolymer with hydrophilic graft chain2014

    • Author(s)
      D. Miyoshi, Y. M. Ueda, N. Shimada, S. I. Nakano, N. Sugimoto, A. Maruyama
    • Journal Title

      ChemMedChem

      Volume: 9 Issue: 9 Pages: 2156-2163

    • DOI

      10.1002/cmdc.201402157

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Lock-and-key mechanism for the controllable fabrication of DNA origami structures2014

    • Author(s)
      R. Arivazhagan, M.Endo, K. Hidaka, N. Shimada, A. Maruyama, H. Sugiyama
    • Journal Title

      Chem. Commun.

      Volume: 59 Issue: 63 Pages: 8743-8746

    • DOI

      10.1039/c4cc02244k

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Intracellular delivery of potential therapeutic genes: prospects in cancer gene therapy2014

    • Author(s)
      A. Bakhtiar, M. Sayyad, R. Rosli, A. Maruyama, E. H. Chowdhury
    • Journal Title

      Curr Gene Ther

      Volume: 14 Issue: 4 Pages: 247-257

    • DOI

      10.2174/1566523214666140612152730

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cationic comb-type copolymer excludes intercalating dye from DNA without inducing DNA condensation2014

    • Author(s)
      A. Maruyama, N. Sonda, K. Yamasaki, S. Kidoaki, N. Shimada, M. Maeshiro, M. Miyazaki
    • Journal Title

      Curr. Nanosci.

      Volume: 10 Issue: 2 Pages: 185-188

    • DOI

      10.2174/1573413709666131129000024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polycation-assisted DNA detection by reduction triggered fluorescence probe towards point-of-care testing2013

    • Author(s)
      H. Saneyoshi, N. Yoshida, N. Shimada, A. Maruyama, Y. Ito, H. Abe
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 23 Issue: 24 Pages: 6851-6853

    • DOI

      10.1016/j.bmcl.2013.10.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of single-nucleotide variations by monitoring the blinking of fluorescence induced by charge transfer in DNA2013

    • Author(s)
      K. Kawai, T. Majima, A. Maruyama
    • Journal Title

      ChemBioChem

      Volume: 14 Issue: 12 Pages: 1430-1433

    • DOI

      10.1002/cbic.201300380

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Promoting strand exchange in a DNA templated transfer reaction2013

    • Author(s)
      J. Michaelis, A. Maruyama, O. Seitz
    • Journal Title

      Chem. Commun.

      Volume: 49 Issue: 6 Pages: 618-620

    • DOI

      10.1039/c2cc36162k

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Polyelectrolyte-Assisted Transconformation of Stem-loop DNA2013

    • Author(s)
      J. Du, L. Wu, N. Shimada, A. Kano, A. Maruyama
    • Journal Title

      Chem. Commun.

      Volume: 49 Issue: 5 Pages: 475-477

    • DOI

      10.1039/c2cc37139a

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reversibly Cross-linked Polymeric Micelles Formed by Autonomously Exchangeable Dynamic Covalent Bonds2013

    • Author(s)
      M. Nishihara, K. Imato, A. Irie, T. Kanehara, A. Kano, A. Maruyama, A. Takahara, H. Otsuka
    • Journal Title

      Chem. Lett.

      Volume: 42 Pages: 377-379

    • NAID

      10031162179

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual crosslinked hydrogel nanoparticles by nanogel bottom-up method for sustained-release delivery2011

    • Author(s)
      Asako Shimoda, et al
    • Journal Title

      Colloids Surf B Biointerfaces

      Volume: (in press) Pages: 38-44

    • DOI

      10.1016/j.colsurfb.2011.09.025

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ureido-derivatized polymers based on both poly(allylurea) and poly(L-citrulline) exhibit UCST-type phase transition behavior under physiologically relevant conditions2011

    • Author(s)
      Shimada, N.; Ino, H.; Maie, K.; Nakayama, M.; Kano, A.; Maruyama, A.
    • Journal Title

      Biomacromolecules

      Volume: 12,(10) Issue: 10 Pages: 3418-22

    • DOI

      10.1021/bm2010752

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Role of Cationic Comb-type Copolymers in Chaperoning DNA Annealing2011

    • Author(s)
      Atsushi Maruyama, et al
    • Journal Title

      Biomaterials

      Volume: 32 Pages: 7671-7676

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Cationic Comb-Type Copolymer Having Tetra-Alkylammonium Groups and its Interaction with DNA2011

    • Author(s)
      Atsushi Maruyama, et al
    • Journal Title

      Current Nanoscience

      Volume: 7-6 Issue: 6 Pages: 979-983

    • DOI

      10.2174/157341311798220600

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Manipulation of lipid bilayer membranes by peptide/cationic copolymer complex2016

    • Author(s)
      Atsushi Maruyama
    • Organizer
      11th International Conference, Medical Applications of Novel Biomaterials and Nanotechnology
    • Place of Presentation
      ペルージア
    • Year and Date
      2016-06-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Design of polymer materials to activate functional DNA and peptides2016

    • Author(s)
      Atsushi Maruyama
    • Organizer
      11th International Sumposium on Polymers Therapeutics from Laboratory to Clinical Practice
    • Place of Presentation
      バレンシア
    • Year and Date
      2016-05-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhancement of DNA enzyme activity by nano-assembling with cationic copolymers2015

    • Author(s)
      Atsushi Maruyama
    • Organizer
      Organic, Inorganic and Hybrid Nanoparticles: Synthesis, Characterization, and Applications (#23), Pacifichem 2015
    • Place of Presentation
      ホノルル
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Interpolyelectrolyte nanoassemly to engineer DNA, peptides, and lipids2015

    • Author(s)
      Atsushi Maruyama
    • Organizer
      Self-assembled Biofunctional Nanomaterials (#433), Pacifichem 2015
    • Place of Presentation
      ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] バイオ分子の個性を輝かせるソフト高分子複合体2015

    • Author(s)
      丸山厚
    • Organizer
      平成27年度日本化学会コロイドおよび界面化学部会先端技術講座
    • Place of Presentation
      東京
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新しい感温性バイオマテリアルとしての側鎖尿素高分子2015

    • Author(s)
      丸山厚
    • Organizer
      附置研究所間アライアンスによるナノとマクロをつなぐ物質・デバイス・システム創製戦略プロジェクト
    • Place of Presentation
      大阪
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] MNAzyme-catalyzed Nucleic Acid Detection Enhanced by a Cationic Copolymer2015

    • Author(s)
      Atsushi Maruyama
    • Organizer
      The 3rd China-Japan Symposium on Nanomedicine
    • Place of Presentation
      北京
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cationic Comb-type Copolymers to Engineer DNA, Peptides and Lipids2014

    • Author(s)
      A. Maruyama
    • Organizer
      JSPS A3 Foresight International Symposium 2014 on Nano-Biomaterials and Regenerative Medicine
    • Place of Presentation
      Tokyo Women’s Medical Univ.
    • Year and Date
      2014-10-08
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Polymer materials to control assembly and functions of biopolymers: DNA, peptide and lipi2014

    • Author(s)
      A. Maruyama
    • Organizer
      The 2nd International Symposium on Polymer Ecomaterials PEM 2014
    • Place of Presentation
      Kunming, China
    • Year and Date
      2014-08-22 – 2014-08-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Soluble inter-polyelectrolyte complexes to engineer assemblies and functions of biopolymers2014

    • Author(s)
      A. Maruyama
    • Organizer
      FRONTIERS OF POLYMER COLLOIDS: FROM SYNTHESIS TO MACRO-SCALE AND NANO-SCALE APPLICATIONS
    • Place of Presentation
      Plague, Czech Republic
    • Year and Date
      2014-07-20 – 2014-07-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enhancement of DNAzyme Activity with Cationic Comb-type Copolymer for Nucleic Acid Detection2014

    • Author(s)
      A. Maruyama
    • Organizer
      The 5th INTERNATIONAL CONFERENCE on the DEVELOPMENT of BIOMEDICAL ENGINEERING in VIETNAM
    • Place of Presentation
      Ho Chi Minh City
    • Year and Date
      2014-06-16 – 2014-06-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Ureido-polymers exhibiting UCST phase behavior under physiological conditions2012

    • Author(s)
      Atsushi Maruyama
    • Organizer
      The 6th International Workshop on Advanced Materials Science And Nanotechnology
    • Place of Presentation
      ベトナム
    • Year and Date
      2012-11-01
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Polymeric materials to manipulate biopolymer assemblies and functions2012

    • Author(s)
      Atsushi Maruyama
    • Organizer
      2012 International Symposium of Materials on Regenerative Medicine
    • Place of Presentation
      台湾
    • Year and Date
      2012-08-29
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Polymer materials to manipulate DNA functions2012

    • Author(s)
      Atsushi Maruyama
    • Organizer
      1st International Symposium on Polymer Ecomaterials (PEM 2012)
    • Place of Presentation
      中国
    • Year and Date
      2012-08-19
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Ureido-polymers Exhibiting UCST-type Phase Transition Behavior under Physiologically Relevant Conditions2012

    • Author(s)
      Atsushi Maruyama
    • Organizer
      4th International Conference "Smart Materials, Structures and Systems”
    • Place of Presentation
      イタリア
    • Year and Date
      2012-06-10
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Annealing activity of cationic comb-type copolymers for DNA assembly2012

    • Author(s)
      丸山厚
    • Organizer
      Spring 2012 National Meeting & Exposition
    • Place of Presentation
      The San Diego Convention Center (America)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内デリバリーを目指した膜破壊性ペプチドの構造・機能制御2012

    • Author(s)
      丸山厚
    • Organizer
      新学術領域研究・ナノメディシン分子科学第2回全体会議
    • Place of Presentation
      京都市国際交流会館(京都)
    • Year and Date
      2012-03-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内核酸イメージングによる細胞機能発現の解明と調節」に関する「ポリ(L-リシン)-グラフト-ポリエチレングリコール共重合体によるポルフィマーナトリウムの腫瘍デリバリー2012

    • Author(s)
      丸山厚
    • Organizer
      新学術領域研究・ナノメディシン分子科学第2回全体会議
    • Place of Presentation
      京都市国際交流会館(京都)
    • Year and Date
      2012-03-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Polymer materials to manipulate biopolymer folding and functions2012

    • Author(s)
      Atsushi Maruyama
    • Organizer
      Emerging Biomaterials 2012
    • Place of Presentation
      韓国
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 「細胞内核酸イメージングによる細胞機能発現の解明と調節」に関する「POLYMER MATERIALS TO MANIPULATE BIOPOLYMER FOLDING AND FUNCTIONS2011

    • Author(s)
      丸山厚
    • Organizer
      FRANCE-JAPAN WORKSHOP
    • Place of Presentation
      Institut Europeen de Chimie et Biologie (Fracne)
    • Year and Date
      2011-10-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内核酸イメージングによる細胞機能発現の解明と調節2011

    • Author(s)
      丸山厚
    • Organizer
      ナノメディシン分子科学第一回全体会議・公開シンポ
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] ペプチドシャペロニング材料による脂質膜構造制御

    • Author(s)
      丸山厚
    • Organizer
      繊維学会西部支部セミナー
    • Place of Presentation
      北九州市立大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] カチオン性高分子によるDNAzymeの活性化

    • Author(s)
      丸山厚
    • Organizer
      平成25年度物質・デバイス領域共同研究拠点特定研究「次世代メディカル・バイオ機能材料への展開を指向した生体分子素子技術の開発」 [B-2] 拡大シンポジウム
    • Place of Presentation
      東北大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cationic Comb-Type Copolymer Enhances DNAzyme Activity

    • Author(s)
      Atsushi Maruyama
    • Organizer
      International Symposium on Smart Biomaterials
    • Place of Presentation
      NIMS, Tsukuba
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2011-08-01   Modified: 2019-07-29  

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