| Budget Amount *help |
¥101,920,000 (Direct Cost: ¥78,400,000、Indirect Cost: ¥23,520,000)
Fiscal Year 2016: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2015: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2014: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥45,760,000 (Direct Cost: ¥35,200,000、Indirect Cost: ¥10,560,000)
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| Outline of Final Research Achievements |
The mechanisms of centrosome duplication have been a long-standing mystery in biology and represent an important open question in biology. Like the genetic material, centrosome duplication occurs once per cell cycle, such that the two resulting centrosomes assemble a bipolar spindle during mitosis, ensuring proper chromosome segregation. We established a molecular basis for the onset of centriole formation by demonstrating that direct association of STIL with Plk4 and subsequent STIL phosphorylation by Plk4 lead to centriolar loading of HsSAS-6 for centriolar cartwheel assembly in human cells. Furthermore, we demonstrated a “swift negative feedback” that the coordinated action of the three key factors can trigger the onset of procentriole formation and, concurrently, ensure formation of a single procentriole per parental centriole. We also demonstrated a mechanism regulating the conversion from premature daughter centrioles to functional mother centrioles in human cells.
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