| Budget Amount *help |
¥109,200,000 (Direct Cost: ¥84,000,000、Indirect Cost: ¥25,200,000)
Fiscal Year 2016: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
Fiscal Year 2015: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2014: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2013: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2012: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Outline of Final Research Achievements |
It has been proposed that RNA virus triggers the membranous replication compartment. In the present study, we identified that COPII and COPI proteins, PREB and Surf4, respectively promotes HCV RNA replication by participating in the formation of the membranous HCV replication compartment and maintaining its proper structure via interacting with NS4B.We also developed the application methods of Atomic Force Microscopy (AFM) to the single molecule structural analysis of long single-stranded RNA molecules, including RNA virus genomes, and the entire structures of HCV genome and full-length 28S rRNA molecule and were successfully obtained. We also succeeded in visualizing the exo- and endo-cytosis processes at cell surface using AFM, which is expected to be useful in further analysis of virus budding processes.
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