Analysis of novel functions of nascent-chain in quality controls of mRNA and protein

• Project Area: Nascent-chain Biology
• Project/Area Number: 26116003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: Inada Toshifumi 東北大学, 薬学研究科, 教授 (40242812)
• Co-Investigator(Kenkyū-buntansha): 長尾 翌手可 東京大学, 大学院工学系研究科(工学部), 助教 (30588017) ; 岩川 弘宙 東京大学, 定量生命科学研究所, 助教 (60710415)
• Project Period (FY): 2014-07-10 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥273,520,000 (Direct Cost: ¥210,400,000、Indirect Cost: ¥63,120,000); Fiscal Year 2018: ¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000); Fiscal Year 2017: ¥50,180,000 (Direct Cost: ¥38,600,000、Indirect Cost: ¥11,580,000); Fiscal Year 2016: ¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000); Fiscal Year 2015: ¥50,180,000 (Direct Cost: ¥38,600,000、Indirect Cost: ¥11,580,000); Fiscal Year 2014: ¥72,540,000 (Direct Cost: ¥55,800,000、Indirect Cost: ¥16,740,000)
• Keywords: 品質管理 / リボソーム / ユビキチン化 / 翻訳異常 / 異常リボソーム / ストレス応答 / 品質管理機構 / 翻訳停止 / プロテアソーム / クライオ電顕 / 翻訳停滞 / 新生鎖 / リボソーム動態 / peptidyl-tRNA / 翻訳伸長制御 / RISC / ユビキチン / タンパク質分解 / mRNA分解 / mRNA品質管理 / 翻訳アレスト

Outline of Final Research Achievements

Accurate gene expression is the basis of life phenomena, and its failure and abnormalities cause various diseases. Cells maintain a quality control mechanism that recognizes and eliminates aberrant translational elongation inhibition. We have analyzed the molecular mechanism that recognizes and dissociates the stalled ribosome. We have elucidated the molecular mechanism by which E3 ubiquitin ligase Hel2/ZNF598 recognizes the specific structure of the stalled ribosome. We also found ribosome modifications that are essential for quality control mechanisms that recognize and eliminate functionally defective ribosomes responsible for various expression abnormalities.

Academic Significance and Societal Importance of the Research Achievements

正確な遺伝子発現はすべての生命現象の根幹であり、その破綻や異常は様々な疾患の原因となる。同義コドンへの置換であるサイレント変異が疾患の原因となることは、翻訳伸長反応の精密な制御が、正確な遺伝子発現に極めて重要であることを示している。本研究により、新生鎖の新規機能と新生鎖の運命決定の分子機構の実体が解明された。本研究の成果により、遺伝子発現の正確性を保証するmRNAとタンパク質の品質管理機構の理解が飛躍的に進んだ。神経細胞における局所翻訳を含む様々な高次生命現象の理解にも大きく貢献するのみでなく、翻訳伸長異常に起因する疾患の発症機構の解明に大きく寄与すると期待される。

Research Products

• [Int'l Joint Research] University of Munich(ドイツ)
• [Int'l Joint Research] University of Munich(Germany)
• [Int'l Joint Research] University of California, Berkeley(米国)
• [Int'l Joint Research] University of Munich(Germany)
• [Int'l Joint Research] University of California, Berkeley(米国)
• [Int'l Joint Research] University of Munich(Germany)
• [Int'l Joint Research] University of California, Berkeley(米国)
• [Int'l Joint Research] PTC Therapeutics(米国)
• [Journal Article] Collided ribosomes form a unique structural interface to induce Hel2‐driven quality control pathways (2019)
  • Author(s): Ikeuchi Ken、Tesina Petr、Matsuo Yoshitaka、Sugiyama Takato、Cheng Jingdong、Saeki Yasushi、Tanaka Keiji、Becker Thomas、Beckmann Roland、Inada Toshifumi
  • Journal Title: The EMBO Journal Volume: 38 Issue: 5
  • DOI: 10.15252/embj.2018100276
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Translation arrest as a protein quality control system for aberrant translation of the 3′ untranslated region in mammalian cells (2019)
  • Author(s): Hashimoto S, Nobuta R, Izawa T, Inada T.
  • Journal Title: FEBS Letters Volume: 1 Issue: 8 Pages: 1-11
  • DOI: 10.1002/1873-3468.13362
  • Peer Reviewed
• [Journal Article] Sequential ubiquitination of ribosomal protein uS3 triggers the degradation of non-functional 18S rRNA. (2019)
  • Author(s): Sugiyama, T., Li, S., Kato, M., Ikeuchi, K., Ichimura, A., Matsuo, Y. and *Inada, T.
  • Journal Title: Cell Reports Volume: 26(12) Issue: 12 Pages: 3400-3415
  • DOI: 10.1016/j.celrep.2019.02.067
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ubiquitination of stalled ribosome triggers ribosome-associated quality control (2017)
  • Author(s): Matsuo Y, Ikeuchi K, Saeki Y, Iwasaki S, Schmidt C, Udagawa T, Sato F, Tsuchiya H, Becker T, Tanaka K, Ingolia NT, Beckmann R, Inada T
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 159-159
  • DOI: 10.1038/s41467-017-00188-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] rucial role of ATP-bound Sse1 in Upf1-dependent degradation of the truncated product. (2017)
  • Author(s): Sugiyama T, Nobuta R, Ando K, Matsuki Y, *Inada, T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 488 Pages: 122-128
  • Peer Reviewed
• [Journal Article] The ribosome as a platform for mRNA and nascent polypeptide quality control (2017)
  • Author(s): Inada, T.
  • Journal Title: Trends Biochem. Sci. Volume: 42 Issue: 1 Pages: 5-15
  • DOI: 10.1016/j.tibs.2016.09.005
  • Peer Reviewed
• [Journal Article] ilencing messages in a unique way (2017)
  • Author(s): Hiro-oki Iwakawa and Yukihide Tomari,
  • Journal Title: Nature Plants, Volume: 3 Issue: 10 Pages: 769-770
  • DOI: 10.1038/s41477-017-0028-2
  • Peer Reviewed
• [Journal Article] Biochemical and single-molecule analyses of the RNA silencing suppressing activity of CrPV-1A. (2017)
  • Author(s): Watanabe M, Iwakawa HO, Tadakuma H, Tomari Y.
  • Journal Title: Nucleic Acids Res. Volume: 45(18) Issue: 18 Pages: 10837-10844
  • DOI: 10.1093/nar/gkx748
  • Peer Reviewed
• [Journal Article] Requirement for eukaryotic translation initiation factors in cap-independent translation differs between bipartite genomic RNAs of red clover necrotic mosaic virus. (2017)
  • Author(s): Tajima Y, Iwakawa HO, Hyodo K, Kaido M, Mise K, Okuno T.
  • Journal Title: Virology Volume: 509 Pages: 152-158
  • DOI: 10.1016/j.virol.2017.06.015
  • Peer Reviewed
• [Journal Article] The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing. (2017)
  • Author(s): Baeg K, *Iwakawa HO, *Tomari Y.
  • Journal Title: Nature Plants Volume: 3 Issue: 4 Pages: 17036-17036
  • DOI: 10.1038/nplants.2017.36
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Conserved functions of human Pelota in mRNA quality control of nonstop mRNA. (2016)
  • Author(s): Ken Ikeuchi, Erina Yazaki, Kazuhei Kudo and Toshifumi Inada
  • Journal Title: FEBS Letters Volume: 590(18) Issue: 18 Pages: 3254-3263
  • DOI: 10.1002/1873-3468.12366
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ribosome-associated Asc1/RACK1 is required for endonucleolytic cleavage induced by stalled ribosome at the 3′ end of nonstop mRNA (2016)
  • Author(s): Ken Ikeuchi, Toshifumi Inada
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 282324-282324
  • DOI: 10.1038/srep28234
  • Peer Reviewed / Open Access
• [Journal Article] FUS regulates AMPA receptor function and FTLD/ALS-associated behaviour via GluA1 mRNA stabilization. (2015)
  • Author(s): Udagawa, T., Fujioka, Y., Tanaka, M., Honda, D., Yokoi, S., Riku, Y., Ibi, D., Nagai, T., Yamada, K., Watanabe, H., Katsuno, M., Inada, T., Ohno, K., Sokabe, M., Okado, H., Ishigaki S*., and Sobue, G.
  • Journal Title: Nat Commun Volume: 6 Issue: 1 Pages: 7098-7098
  • DOI: 10.1038/ncomms8098
  • Peer Reviewed / Open Access
• [Journal Article] The tRNA splicing endonuclease complex cleaves the mitochondria-localized CBP1 mRNA. (2015)
  • Author(s): Tsuboi, T., Yamazaki, R., Nobuta, R., Ikecuhi, K., Makino, S., Ohtaki, Y., Suzuki, Y., Yoshihisa, T., Trotta, C., and Inada, T.
  • Journal Title: Journal of Biological Chemistry Volume: 290 Issue: 26 Pages: 16021-16030
  • DOI: 10.1074/jbc.m114.634592
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protein quality control systems associated with no-go and nonstop mRNA surveillance in yeast. (2014)
  • Author(s): Matsuda R, Ikeuchi K, Nomura S, Inada T.
  • Journal Title: Genes Cells. Volume: 19 Issue: 1 Pages: 1-12
  • DOI: 10.1111/gtc.12106
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Profiles of extracellular miRNAs in the aqueous humor of glaucoma patients assessed with a microarray system. (2014)
  • Author(s): Tanaka Y, Tsuda S, Kunikata H, Sato J, Kokubun T, Yasuda M, Nishiguchi KM, Inada T, Nakazawa T.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 5089-5089
  • DOI: 10.1038/srep05089
  • Peer Reviewed / Open Access
• [Journal Article] MicroRNAs block assembly of eIF4F translation initiation complex in Drosophila (2014)
  • Author(s): #Takashi Fukaya, #Hiro-oki Iwakawa, *Yukihide Tomari, (#同等貢献)
  • Journal Title: Molecular Cell Volume: 56 Issue: 1 Pages: 67-78
  • DOI: 10.1016/j.molcel.2014.09.004
  • Peer Reviewed
• [Presentation] General role of ribosome ubiquitination in quality controls induced by ribosome stalling (2019)
  • Author(s): 稲田利文
  • Organizer: RIBOSOME Structure and Function 2019
  • Int'l Joint Research / Invited
• [Presentation] Vms1 is a cytosolic CAT-tailing antagonist to protect mitochondria (2019)
  • Author(s): 井澤俊明
  • Organizer: 第20回武田科学振興財団生命科学シンポジウムRNAネオバイオロジー
  • Int'l Joint Research
• [Presentation] mRNA cleavages at the leading and colliding stalled ribosomes depend on the ribosome-associated quality control trigger machinery (2019)
  • Author(s): 池内健
  • Organizer: 第20回武田科学振興財団生命科学シンポジウムRNAネオバイオロジー
  • Int'l Joint Research
• [Presentation] Hydrophobicity dependent translation arrest prevents production of 3'UTR derived aberrant products (2019)
  • Author(s): 橋本怜史
  • Organizer: 第20回武田科学振興財団生命科学シンポジウムRNAネオバイオロジー
  • Int'l Joint Research
• [Presentation] Sequential ribosome ubiquitylation leads to 18S Nonfunctional Ribosomal RNA Decay (2019)
  • Author(s): 杉山誉人
  • Organizer: 第20回武田科学振興財団生命科学シンポジウムRNAネオバイオロジー
  • Int'l Joint Research
• [Presentation] Ubiquitination of ribosomal protein uS3 triggers the subunit dissociation leadinag to degradation of nonfunctional 40S subunit (2019)
  • Author(s): 李 思涵
  • Organizer: 第20回武田科学振興財団生命科学シンポジウムRNAネオバイオロジー
  • Int'l Joint Research
• [Presentation] General roles of ribosome ubiquitination and RQT-dependent subunit dissociation in quality controls for aberrant translation elongation (2018)
  • Author(s): 稲田利文
  • Organizer: Translational Control
  • Int'l Joint Research
• [Presentation] 翻訳品質管理におけるリボソーム修飾の普遍的機能 (2018)
  • Author(s): 稲田利文
  • Organizer: 第５回Ribosome meeting
• [Presentation] サイトゾルタンパク質Vms1による新生ミトコンドリアタンパク質の品質管理機構 (2018)
  • Author(s): 井澤俊明
  • Organizer: 第５回Ribosome meeting
• [Presentation] 小胞体ストレス応答におけるリボソームユビキチン化の新規機能 (2018)
  • Author(s): 稲田利文
  • Organizer: 第１３回小胞体ストレス研究会
• [Presentation] 小胞体ストレス応答におけるリボソームユビキチン化による新規翻訳制御機構 (2018)
  • Author(s): 松木泰子
  • Organizer: 第１３回小胞体ストレス研究会
• [Presentation] NOT4によるリボソームユビキチン化の生理的意義の解析 (2018)
  • Author(s): 松木泰子
  • Organizer: 第６回CCR4-NOT研究会
• [Presentation] Multiple-step ubiquitination of stalled ribosomes induces endonucleolytic cleavages of mRNA (2018)
  • Author(s): 稲田利文
  • Organizer: Ubiquitin and Cellular Regulation
  • Int'l Joint Research
• [Presentation] 3’UTR の異常翻訳に起因する品質管理機構の解析 (2018)
  • Author(s): 橋本怜史
  • Organizer: 第20回日本RNA学会年会
• [Presentation] 多段階ユビキチン化反応が機能不全リボソームの分解を誘導する (2018)
  • Author(s): 杉山誉人
  • Organizer: 第20回日本RNA学会年会
• [Presentation] 機能欠失リボソームの品質管理機構18S NRDの上流経路の解析 (2018)
  • Author(s): 李 思涵
  • Organizer: 第20回日本RNA学会年会
• [Presentation] 翻訳伸長停滞に起因するmRNA品質管理機構No-Go Decayは多段階のリボソームユビキチン化によって制御される (2018)
  • Author(s): 池内健
  • Organizer: 第20回日本RNA学会年会
• [Presentation] トリプトファン連続配列に起因する品質管理機構の解析 (2018)
  • Author(s): 水野雅人
  • Organizer: 第20回日本RNA学会年会
• [Presentation] 機能欠失リボソーム品質管理機構18S NRDにおいて誘導されるE3ユビキチンライゲースのスクリーニング (2018)
  • Author(s): 加藤海輝
  • Organizer: 第20回日本RNA学会年会
• [Presentation] Multi-step ubiquitylation of stalled ribosomes evokes endoribonucleolytic cleavages of mRNA in no-go decay (2018)
  • Author(s): 池内健
  • Organizer: 国際シンポジウム“International symposium Protein: From the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Sequential ribosome ubiquitylation induces Nonfunctional Ribosomal RNA Decay (2018)
  • Author(s): 杉山誉人
  • Organizer: 国際シンポジウム“International symposium Protein: From the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Sequential ubiquitination of ribosomal protein uS3 triggers the subunit dissociation leading to degradation of nonfunctional 40S subunit (2018)
  • Author(s): 李 思涵
  • Organizer: 国際シンポジウム“International symposium Protein: From the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Mechanistic insight into quality control system induced by aberrant translation of 3’UTR (2018)
  • Author(s): 橋本怜史
  • Organizer: 国際シンポジウム“International symposium Protein: From the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] Consecutive tryptophan sequence induces Rqc2-independent RQC (2018)
  • Author(s): 水野雅人
  • Organizer: 国際シンポジウム“International symposium Protein: From the Cradle to the Grave”
  • Int'l Joint Research
• [Presentation] 品質管理と翻訳制御における3‘非翻訳領域の新規機能 (2018)
  • Author(s): 稲田利文
  • Organizer: 第１８回日本蛋白質科学会年会
• [Presentation] Disome as a structural unit for quality control pathways induced by translation arrest (2018)
  • Author(s): 池内健
  • Organizer: 第４１回日本分子生物学会年会
• [Presentation] 機能欠失リボソーム分解系18S NRDを誘導するリボソームユビキチン化反応の解析 (2018)
  • Author(s): 加藤海輝
  • Organizer: 第４１回日本分子生物学会年会
• [Presentation] 翻訳停滞に起因するタンパク質品質管理因子は正常な神経突起伸長と認知機能に必須である (2018)
  • Author(s): 奥山卓
  • Organizer: 第４１回日本分子生物学会年会
• [Presentation] Analysis of ribosome-associated quality control (RQC) regulation by deubiquitination enzyme Ubp2 (2018)
  • Author(s): 友松翔太
  • Organizer: RNAフロンティアミーティング２０１８
• [Presentation] 未成熟18S rRNAの制御機構の解析 (2018)
  • Author(s): 庄内王功人
  • Organizer: RNAフロンティアミーティング２０１８
• [Presentation] 小胞体ストレス応答に関与するE3酵素の探索 (2018)
  • Author(s): 中野裕
  • Organizer: RNAフロンティアミーティング２０１８
• [Presentation] 品質管理で分解されないITS2非切断異常27SB rRNAを含む60S サブユニットは、細胞増殖阻害を引き起こす (2018)
  • Author(s): 林涼也
  • Organizer: RNAフロンティアミーティング２０１８
• [Presentation] 哺乳類神経細胞におけるRQC(Ribosome-associated quality control)因子の解析 (2018)
  • Author(s): 関萌香
  • Organizer: RNAフロンティアミーティング２０１８
• [Presentation] 未成熟18S rRNAの制御機構の解析 (2018)
  • Author(s): 庄内 王功人
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 品質管理で分解されないITS2 非切断異常27SB rRNAを含む60S サブユニットは、細胞増殖阻害を引き起こす (2018)
  • Author(s): 林 涼也
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 翻訳停滞に起因したタンパク質品質管理機構RQCの内在性標的TRQ1の解析 (2018)
  • Author(s): 中島 静香
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 機能欠失リボソームの品質管理機構18SNRDにおけるサブユニット解離の誘導経路に関する解析 (2018)
  • Author(s): 李 思涵
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] トリプトファン連続配列に起因するタンパク質品質管理機構RQCの解析 (2018)
  • Author(s): 水野 雅人
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 合成途中の新生ポリペプチド鎖の分解におけるNot4の機能解析 (2018)
  • Author(s): 西尾 聡一郎
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 小胞体ストレス応答に関与するE3酵素の探索 (2018)
  • Author(s): 中野裕
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 脱ユビキチン化酵素Ubp2によるRQCの制御機構の解析 (2018)
  • Author(s): 友松翔太
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 機能欠失リボソーム分解系を誘導する多段階ユビキチン化反応の解析 (2018)
  • Author(s): 加藤 海輝
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] フレームシフトによる異常ユビキチンの産生機構 (2018)
  • Author(s): 佐藤慎吉
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 哺乳類神経細胞におけるRQC(Ribosome-associated quality control)因子の解析 (2018)
  • Author(s): 関 萌香
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 3’-UTR由来翻訳産物の探索と活性解析 (HDAC1遺伝子とPD-L1 遺伝子に関する解析) (2018)
  • Author(s): 鳥海 靖人
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 翻訳停滞に起因するタンパク質品質管理因子は正常な神経突起伸長と認知機能に必須である。 (2018)
  • Author(s): 奥山 卓
  • Organizer: 第57回日本薬学会東北支部大会
• [Presentation] 翻訳品質管理におけるリボソームユビキチン化の普遍的機能 (2018)
  • Author(s): 稲田利文
  • Organizer: ユビキチン研究会
• [Presentation] 品質管理機構NGDにおけるリボソームユビキチン化の多段階機能 (2018)
  • Author(s): 池内 健
  • Organizer: ユビキチン研究会
• [Presentation] A Novel Complex Targets Ubiquitinated Ribosome to Ribosome Quality Control (2017)
  • Author(s): 稲田利文
  • Organizer: Protein quality control: Success and failure in health and disease
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanism of biological control and information conversion by RNA (2017)
  • Author(s): 稲田利文
  • Organizer: 第69回日本細胞生物学会大会
• [Presentation] Ubiquitination of uS10 by Hel2 and RQT complex triggers Ribosome-associated uality Control (2017)
  • Author(s): 稲田利文
  • Organizer: Summer School Dynamics of Translation
  • Int'l Joint Research / Invited
• [Presentation] 翻訳停滞に起因するmRNA分子内切断NGDとタンパク質品質管理機構RQCの相関解析 (2017)
  • Author(s): 池内 健
  • Organizer: 日本RNA学会年会
• [Presentation] ナンセンス変異依存異常mRNA由来の短鎖型タンパク質分解促進機構の解析 (2017)
  • Author(s): 杉山 誉人
  • Organizer: 日本RNA学会年会
• [Presentation] リボソームユビキチンコードによる翻訳品質管理の分子機構 (2017)
  • Author(s): 稲田利文
  • Organizer: 酵母遺伝学フォーラム第50回研究会報告会
• [Presentation] 3’非翻訳領域（UTR)におけるnon-AUG dependent translationの解析 (2017)
  • Author(s): 佐藤 光紗希
  • Organizer: 第56回日本薬学会東北支部大会
• [Presentation] 翻訳停滞に起因するmRNA/タンパク質品質管理因子の神経細胞における生理的機能の解析 (2017)
  • Author(s): 奥山 卓
  • Organizer: 第56回日本薬学会東北支部大会
• [Presentation] フレームシフトによる異常ユビキチンの産生機構 (2017)
  • Author(s): 佐藤 慎吉
  • Organizer: 第56回日本薬学会東北支部大会
• [Presentation] 神経特異的遺伝子抑制因子CoREST/HDAC1の3‘UTR IRES 活性の解析 (2017)
  • Author(s): 鳥海 靖人
  • Organizer: 第56回日本薬学会東北支部大会
• [Presentation] 品質管理因子RQT複合体の相互作用解析 (2017)
  • Author(s): 西尾 聡一郎
  • Organizer: 第56回日本薬学会東北支部大会
• [Presentation] Novel regulatory roles of ribosomes in global gene expression (2016)
  • Author(s): 稲田利文
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2016-11-30
• [Presentation] リボソーム修飾シグナルによる翻訳の動的制御 (2016)
  • Author(s): 稲田利文
  • Organizer: 第4回RIBOSOME MEETING
  • Place of Presentation: 大阪医科大学（大阪府）
  • Year and Date: 2016-09-17
• [Presentation] Mechanistic insight into recognition and dissociation of stalled ribosome to induce ribosome-associated quality control system (2016)
  • Author(s): 松尾芳隆
  • Organizer: 第4回RIBOSOME MEETING
  • Place of Presentation: 大阪医科大学（大阪府）
  • Year and Date: 2016-09-17
• [Presentation] Crucial roles of ribosome ubiquitination in quality controls and stress respone (2016)
  • Author(s): 松尾芳隆
  • Organizer: The 14th International Congress on Yeasts ; ICY2016
  • Place of Presentation: 淡路夢舞台国際会議場 （兵庫県）
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] Ubiquitination of stalled ribosome triggers ribosome-associated quality control (2016)
  • Author(s): 稲田利文
  • Organizer: Translational Control 2016
  • Place of Presentation: Cold Spring Harbor Laboratory(NY)
  • Year and Date: 2016-09-06
  • Int'l Joint Research
• [Presentation] Crucial roles of ribosome ubiquitination in quality controls and stress response (2016)
  • Author(s): 稲田利文
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Functional analysis of RQC-trigger (RQT) factors in mammalian cells (2016)
  • Author(s): 宇田川剛
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Functional analysis of ribosome ubiquitination on the quality controls of nonfunctional ribosomes (2016)
  • Author(s): 市村淳
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Crucial roles of down-regulation of de-ubiquitynation complex Ubp3-Bre5 in translation attenuation by ER etress (2016)
  • Author(s): 横尾秀幸
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] The rotated ribosome with hybrid two tRNAs as a crucial intermediate for translocation,subunit dissociation and frameshift (2016)
  • Author(s): 坂本一真
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Novel quality controls induced by translation of 3' UTR (2016)
  • Author(s): 佐藤光紗希
  • Organizer: NASCENT CHAIN BIOLOGY 2016
  • Place of Presentation: 富士レークホテル（山梨県）
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] T25.Ubiquitylation of ribosomal proteins play crucial roles in quality controls induced by aberrant translation (2016)
  • Author(s): 稲田利文
  • Organizer: Post-transcriptional control of gene expression:Mechanisms of RNA decay
  • Place of Presentation: Lisbon Marriott Hotel （ポルトガル）
  • Year and Date: 2016-07-10
  • Int'l Joint Research
• [Presentation] Ubiquitylation of Stalled Ribosome Triggers Ribosome Quality Control (2016)
  • Author(s): 稲田利文
  • Organizer: Ribosome Structure and Function 2016
  • Place of Presentation: Strasbourg Convention Centre（フランス）
  • Year and Date: 2016-07-06
  • Int'l Joint Research
• [Presentation] Ribosome-associated quality control(RQC)requires the ubiquitylation of the ribosomal protein uS10 and novel RQC-triggering factors (2016)
  • Author(s): 稲田利文
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research / Invited
• [Presentation] Identification of novel factors involved in the primary step to induce ribosome-associated quality control system (2016)
  • Author(s): 松尾芳隆
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Ubiquitylation of stalled ribosome triggers ribosome-mediated quality control system (2016)
  • Author(s): 池内健
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] The role of ribosome ubiquitylation in translation attenuation during ER stress in yeast (2016)
  • Author(s): 松木泰子
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Conserved role of E3 ubiquitin ligases in ribosome-associated quality control (2016)
  • Author(s): 橋本怜史
  • Organizer: RNA2016
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Ubiquitylation of ribosomal protein uS10 is crucial for ribosome-associated quality control (2016)
  • Author(s): 稲田利文
  • Organizer: UBIQUITIN&CELLULAR REGULATION
  • Place of Presentation: Big Sky Resort Road Big Sky( MT)USA
  • Year and Date: 2016-06-12
  • Int'l Joint Research
• [Presentation] Ubiquitylation of stalled ribosome triggers ribosome quality control (2016)
  • Author(s): 稲田利文
  • Organizer: Protein Homeostasis in Health and Disease
  • Place of Presentation: Cold Spring Harbor Laboratory(NY)USA
  • Year and Date: 2016-04-18
  • Int'l Joint Research
• [Presentation] Co-translational quality controls of mRNA and protein (2016)
  • Author(s): 稲田利文
  • Organizer: RNA2016 satellite-symposium Nascent biology and Ribosome functions
  • Place of Presentation: 京都大学医学部創立百周年記念施設 芝蘭会館
• [Presentation] Ubiquitylation of Stalled Ribosome Triggers Ribosome Quality Control (2015)
  • Author(s): 稲田利文
  • Organizer: 第38回日本分子生物学会年会 第88回日本生化学大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 肝臓におけるCNOT8によるEgfr発現制御機構の解析 (2015)
  • Author(s): 松木 泰子
  • Organizer: 第38回日本分子生物学会年会 第88回日本生化学大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] NRDにおけるリボソームのユビチキン化の 機能解析 (2015)
  • Author(s): 市村 淳
  • Organizer: 第38回日本分子生物学会年会 第88回日本生化学大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 翻訳伸長異常に起因する品質管理機構の新展開 (2015)
  • Author(s): 稲田利文
  • Organizer: 第10回臨床ストレス応答学会大会
  • Place of Presentation: 東京農工大学140周年記念会館
  • Year and Date: 2015-11-07
  • Invited
• [Presentation] Ubiquitylation of Stalled Ribosome Triggers Ribosome Quality Control (2015)
  • Author(s): Toshifumi Inada
  • Organizer: Nascent-chain Biology Meeting 2015 in Tokyo
  • Place of Presentation: 東京大学一条ホール
  • Year and Date: 2015-10-01
• [Presentation] 翻訳伸長阻害に起因するRQCと遺伝性疾患関連遺遺伝子の解析 (2015)
  • Author(s): 信田 理沙
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 岩手医科大学矢巾キャンパス
  • Year and Date: 2015-09-26
• [Presentation] 肝臓におけるCNOT8によるEgfr発現制御機構の解析 (2015)
  • Author(s): 松木 泰子
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 岩手医科大学矢巾キャンパス
  • Year and Date: 2015-09-26
• [Presentation] NRDにおけるリボソームのユビチキン化の機能解析 (2015)
  • Author(s): 市村 淳
  • Organizer: 第54回日本薬学会東北支部大会
  • Place of Presentation: 岩手医科大学矢巾キャンパス
  • Year and Date: 2015-09-26
• [Presentation] Ubiquitylation of Ribosomal Proteins Play Crucial Roles in Translation Surveillance Systems (2015)
  • Author(s): Toshifumi Inada
  • Organizer: Ubiquitin and ubiquitin-like modifiers
  • Place of Presentation: HOTEL CROATIA CAVTAT,Dubrovnik,Croatia
  • Year and Date: 2015-09-18
  • Int'l Joint Research
• [Presentation] Ubiquitylation of ribosomal proteins governs the fate of stalled ribosomes (2015)
  • Author(s): Toshifumi Inada
  • Organizer: Protein Synthesis and Translational Control
  • Place of Presentation: European Molecular Biology Laboratory, Heidelberg, Germany
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] Novel roles of ribosome ubiquitination in quality control systems (2015)
  • Author(s): Toshifumi Inada
  • Organizer: Genetic code and translation
  • Place of Presentation: Hotel & Internationales Congress Center Dresden,Germany
  • Year and Date: 2015-07-17
  • Int'l Joint Research / Invited
• [Presentation] 肝臓におけるCNOT8によるEgfr発現制御機構の解析 (2015)
  • Author(s): 松木 泰子
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: ホテルライフォート札幌（札幌市）
  • Year and Date: 2015-07-15
• [Presentation] NRDにおけるE3ユビチキン化酵素He12の機能解析 (2015)
  • Author(s): 市村 淳
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: ホテルライフォート札幌（札幌市）
  • Year and Date: 2015-07-15
• [Presentation] 翻訳伸長複合体の運命決定機構におけるリボソームユビキチン化の新規機能 (2015)
  • Author(s): 稲田利文
  • Organizer: 第67回 日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀（東京）
  • Year and Date: 2015-06-30
  • Invited
• [Presentation] 翻訳伸長複合体の運命決定機構 (2014)
  • Author(s): 稲田 利文
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Ubiquitylation of ribosomal proteins governs the fate of stalled ribosomes (2014)
  • Author(s): Toshifum Inada
  • Organizer: Joint Australia and Japan RNA meeting
  • Place of Presentation: University of Technology,Sydney,Australia
  • Year and Date: 2014-11-02 – 2014-11-05
  • Invited
• [Presentation] 翻訳伸長複合体の品質管理機構におけるユビキチン化の新規機構 (2014)
  • Author(s): 稲田 利文
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Hel2 induces ribosome quality control by ubiquitination of 40S ribosomal proteins in stalled ribosomes (2014)
  • Author(s): Toshifum Inada
  • Organizer: Translational Control
  • Place of Presentation: Cold Spring Harbor Laboratory,NY,USA
  • Year and Date: 2014-09-02 – 2014-09-06
  • Invited
• [Presentation] Ribosome assosiated E3 ubiquitin ligase complex is required for mRNA and protein quality control systems induced by translation arrest (2014)
  • Author(s): Toshifum Inada
  • Organizer: Post-Transcriptional Control of Gene Expression: Mechanism of mRNA Decay
  • Place of Presentation: BigSky,Montana,USA
  • Year and Date: 2014-07-06 – 2014-07-11
• [Book] Novel roles of the multi-functional CCR4-NOT complex in post-transcriptional regulation. (2014)
  • Author(s): Inada T, Makino S.
  • Total Pages: 7
  • Publisher: Front Genet
• [Remarks] 東北大学大学院薬学研究科遺伝子制御薬学分野
  • URL: http://www.pharm.tohoku.ac.jp/~idenshi/inada_lab_HP/Home.html
• [Remarks] 東京大学分子細胞生物学研究所RNA機能研究分野
  • URL: http://www.iam.u-tokyo.ac.jp/tomari/
• [Remarks] 東京大学大学院工学系研究科 化学生命工学専攻RNA生命化学
  • URL: http://rna.chem.t.u-tokyo.ac.jp
• [Funded Workshop] Nascent-chain biology meeting 2015 (2015)
  • Place of Presentation: 蔵王、東京大学弥生会館
  • Year and Date: 2015-09-28