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Analysis of wound healing mechanism at cellular and molecular levels

Research Project

Project/Area Number 01480316
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKeio university

Principal Investigator

FUJINO Toyomi  Keio University School of Medicine, Dept. Plastic Surgery, Professor, 医学部, 教授 (20051279)

Co-Investigator(Kenkyū-buntansha) MINABE Toshiharu  Keio University School of Medicine, Dept. Plastic Surgery, Instructor, 医学部, 助手 (50200077)
KOBAYASHI Masahiro  Keio University School of Medicine, Dept. Plastic Surgery, Instructor, 医学部, 助手 (30195812)
SEGAWA Kaoru  Keio University School of Medicine, Dept. Microbiology, Assistant Professor, 医学部, 講師 (30114523)
TAKANO Toshiya  Keio University School of Medicine, Dept. Microbiology, Professor, 医学部, 教授 (60051364)
今西 宣晶  慶應義塾大学, 医学部, 助手 (00184820)
栗原 卓也  慶應義塾大学, 医学部, 助手 (90186510)
長谷川 時生  慶應義塾大学, 医学部・形成外科, 助手 (00180860)
福積 聡  慶應義塾大学, 医学部・形成外科, 助手 (90181289)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsWound healing / Keloid / Collagen synthesis / TGF-beta / Collagenase gene / Immortalization / Collagen mRNA / Regulation of cell proliferation / 創傷治癒 / 過形成 / 細胞異常増殖 / コラ-ゲン / 血管新生 / ヒトニ倍体細胞 / トランスフォ-メ-ション / SV40 largeT抗原 / 細胞不死化 / 染色体異常 / 細胞増殖速度 / 分子生物学 / プロコラ-ゲンα1(I)mRNA
Research Abstract

Procollagen ccl, mRNA was specifically expressed at higher levels in the experimental wound tissue on rat dorsal skin five to seven days after the injury. TGF-beta induced the expression of procollagen alphal MRNA at similar levels in the fibroblasts cultured from normal human skin and keloidal tissues. The incorporation of ^<14>C-leucine was three to five more in human fresh keloidal slices than those of normal dermis, suggesting that the hyperproduction of collagen was regulated at a translational level in keloid. The serum of patients with systemic severe keloid strongly stimulated the growth of cultured skin fibroblasts compared with normal human and fetal calf sera. Both higher concentration of growth-stimulating factors and activation of collagen production at a translational level seemed to be involved in the pathogenesis of keloid. In the study on the immortalization of SV40-transformed human diploid fibroblasts as an in vitro modpl of the abnormal proliferation in keloidal skin fibroblasts, certain mutational events responsible for immortalization occurred in crisis and the expression of collagenase gene was specifically shut off after immortalization. The synthesis and degradation of collagen seemed to be intrinsically related with the growth regulation in fibroblasts. In experimental skin pedicle flaps, we detected the prominent dilatation of small anastomotic arteries which seemed to play major roles in supplying blood cells releasing wound-healing factors rather than angiogenesis.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 今西 宣晶,他: "肩胛骨付分割肩胛皮弁による下顎骨再建の経験" 形成外科. 33. 349-354 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤野 豊美,他: "シュミレ-ション外科の進歩" 第23回日本医学会総会会誌. III. 202-203 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujino,T.,et al.: "A free full-thickness calvarila bone graft to reconstruct a post-mandibular fracture deformity:A case with 19 years follow-up." Eur.J.Plast.Surg.14. 250-251 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kato,S.,et al.: "Genetic recombination in a chromosomal translocation t(2;9)(p11;q24) of a Burkitt's lymphoma cell line,KOBK101." Gene. 97. 239-244 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Umezawa,A.,et al.: "Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor." Mol.Cell.Biol.11. 920-927 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Imai,S.,et al.: "Immortalization of human diploid fibroblasts transformed by SV40 large T antigen:A key event responsible for immortalization occurs during crisis." Exp.Cell.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujino, T., Kaneko, T., Nakajima, H., Kurihara, T., Kobayashi, M. and Chiyokura, H.: "Progresses in Simulation Surgery (in Japanese)" Abst. 23rd Meet. Japan. Med. Assoc.III. 202-203 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujino, T., Tajima, S. and Nakajima, H.: "A Free Full-Thickness Calvarila Bone Graft to Reconstruct a Post-Mandibular Fracture Deformity : A Case with 19 Years Follow-up." Eur. J. Plast. Surg.14. 250-251 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Umezawa, A., Tachibana, K., Harigaya, K. . Kusakari, S., Kato, S., Watanabe, Y. and Takano, T.: "Colony-Stimulating Factor I Expression is Down-Regulated During the Adipocyte Differentiation of H-1/A Marrow Stromal Cells and Induced by Cachectin/Tumor Necrosis Factor." Mol. Cell. Biol.11. 920-927 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Imai, S., Saito, F., Ikeuchi, T., Segawa, K. and Takano, T.: "Immortalization of Human Diploid Fibroblasts Transformed by SV40 Large T Antigen : A Key Event Responsible for Immortalization Occurs During Crisis." Exp. Cell. Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujino,T.et al.: "A free Fullーthickness calvarila bone graft to reconstruct a postーmandibular fracture deformity:A case with 19 yars folowーup" Eur.J.Plast.Surg.14. 250-251 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤野 豊美,他: "シュミュレ-ション外科の進歩" 第23回日本医学会総会会誌. 3. 202-203 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kato,S.,Takano,T.et al.: "Genetic recombination in a chromosomal thranslocation t(2;9)(p11;924)of a Burkitt's lymphoma cell line,KOBK 101." Gene. 97. 239-244 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Umezawa,A.,Takano,T et al.: "Colonyーstimulating factor 1 expression is downregulated during the adipocyte differentiation of Hー1/A marrow stromal cells and induced by cachectin/tumor necrosis factor." Mol.Cell.Biol.11. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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