• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cytopathological studies of Ewing's sarcoma and an application of pathologic diagnosis using newly produced monoclonal antibodies

Research Project

Project/Area Number 01570169
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionHokkaido University

Principal Investigator

NOJIMA Takayuki  Hokkaido University, School of Medicine, lecturer, 医学部, 講師 (50142732)

Co-Investigator(Kenkyū-buntansha) ABE Syuuichi  Hokkaido University, Faculty of Science, Associate Professor, 理学部, 助教授 (80125278)
NAGASHIMA Kazuo  Hokkaido University, School of Medicine, Professor, 医学部, 教授 (50010377)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsEwing's sarcoma / Neuron-specific enolase / Neuroectoderm / Neuroblastoma / Monoclonal antibody / Samll cell osteosarcoma / Cytopathology / 骨腫瘍 / 組織診断学
Research Abstract

1. Three human Ewing's sarcoma lines were established, and these were investigated for their morphological and cytogenetic characterization and molecular analysis.
2. Chromosome analysis revealed a translocation, t(11 ; 22)(q24 ; q12)in two of three lines, as reported previously for Ewing's sarcoma and primitive neuroectodermal tumors of bones and soft tissues.
3. Morphology of cell cultures was unaffected by exposure to dbcAMP. Values of neuron-specific enolase were high in cultured cells, supematant of the culture fluid, and nude mice serum.
4. Two clones of monoclonal anti-Seki antibodies were produced using the classical hybridoma technology. Expression of these target antigens was found in tumor cells of Ewing's sarcoma, neuroblastoma and osteosarcoma among tumor cells of Ewing's sarcoma, neuroblastoma, osteosarcoma rhabdomyosarcoma, malignant lymphoma and other carcinomas examined.
5. The findings of Southem blot and Northern blot analyses of the N-myc, C-myc, Nras, ets-1 and PDGFB oncogenes were not specific for Ewing's sarcoma.
6. We compared Ewing's sarcoma with rbabdomyosarcoma and small cell osteosarcoma by morphological, immunobistochemical and cytogenetic studies. The morphological features of neuroblastoma is easily confused with Ewing's sarcoma by light microscopy and immunohistochemistry. We established a new neuroblastoma cell line(HNB- 18)and investigated a difference between neuroblastoma and Ewing's sarcoma. These results described above(1-6)suggest that Ewing's sarcoma may originate in a primitive neuroectodermal cell. Although a difference between Ewing's sarcoma and primitive neuroectodermal tumor is still unclear from our work, we consider that these two tumors may be expressed by the histological grade of differentiation.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report

Research Products

(18 results)

All Other

All Publications (18 results)

  • [Publications] 野島 孝之,他: "ユ-イング肉腫株Sekiの樹立と性状" Human Cell. 2. 192-193 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 畑江 芳郎,他: "小児期のEwing肉腫" 小児がん. 25. 479-481 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 山口 秀夫,他: "骨盤発生のEwing肉腫" 整形外科. 42. 457-463 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 野島 孝之: "小細胞骨肉腫の病理と鑑別診断" 病理と臨床. 9. 321-325 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima.T. ea al.: "Morphological and cytogenetic characterization and Nthyc onlogene analysis of a newly established neuroblastoma cell line" Acta Pathol. Jpn. 41. 507-515 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima.T.et al.: "Morphological and cytogenetic studies of Ewing sarcoma" 発表予定.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima, T. et al.: "Estabilishment and characterization of Seki cell line from an Ewing's sarcoma." Human Cell. 2. 192-193 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hatae, Y. et al.: "Ewing's sarcoma in childhood." Pediatric Oncology. 25. 479-481 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamaguchi, H. et al.: "Ewing's sarcoma of the pelvis." Seikeigeka. 42. 457-463 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima, T.: "Small cell osteosarcoma and its differential diagnosis." Pathology and Clinical Medicine. 9. 321-325 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima, T. et al.: "Morphological and cytogenetic characterization and N-myc oncogene analysis of a newly established neuroblastoma cell line." Acta Pathol. Jpn.41. 507-515 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nojima, T. et al.: "Morphological and cytogenetic studies of Ewing's sarcoma."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayuki Nojima,et al.: "Morphological and cytogenetic characterization and Nーmyc onccgene analysis in a naly established neuroblastoma cell line" Acta Pathol Jpn. 41. 507-515 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takayuki Nojima,et al.: "Morphslogical and cytogenetic studies of Ewing's sarcoma"

    • Related Report
      1991 Annual Research Report
  • [Publications] Takayuki Nojima: "A case of alveolar rhabdomyosarcoma with a chromosomal translocation,t(2;13)(q37;q14)" Virchows Archiv A Pathological Anatomy and Histopathology. 417. 357-359 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 野島 孝之: "小細胞骨肉腫の病理と鑑別診断" 病理と臨床. 9. 321-325 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takayuki Nojima: "Mcrphological and Cytogenetic characterization and Nーmyc oncogene analysis in a newly established neuroblastoma cell line" Acta Pathologica Japonica.

    • Related Report
      1990 Annual Research Report
  • [Publications] Takayuki Nojima: "Establishment and characterization of a human neuroblastoma cell line and its heterotransplantation into nude mice" Human Cell.

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi