• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on Expression and Amplification of C-Oncogenes in Trophoblastic Diseases

Research Project

Project/Area Number 01570942
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNippon Medical School

Principal Investigator

SASAKI Shigeru M. D. Ph. D.  Nippon Medical School Obstetrics & Gynecology Associate Professor, 医学部, 助教授 (60089737)

Co-Investigator(Kenkyū-buntansha) 野村 信夫  日本医科大学老人病研究所, 助教授 (20147862)
NOMURA Nobuo Ph. D.  Nippon Veterinary & Zootechnical College Molecular Oncology Associate Professor
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsTrophoblastic disease / Hydatidiform mole / Choriocarcinoma / Oncogene / Expression / Amplification / DNA / mRNA / oncogene / expression / amplification / choriocarcinoma cell line / trophoblastic diseases / Hydatiform nule / amplitication
Research Abstract

As a first step in an ongoing study on correlation of oncogene expression and tumorigenesis in trophoblastic diseases. We determined which oncogenes were expressed in hydatidiform mole and choriocarcinoma cell lines. Southern blot hybridization and Northern blot hybridization were done with eighteenkinds of oncogene probes. No rearrangement and amplification of oncogenes were noted in fresh hydatidiform mole. But, when we checked the localization of myconcogen product at light microscopic level by avidine-biotin technique using the monoclonal antibody in the mole, strong expression of myc product was detected in cytoplasm of molar trophoblast compared with normal villi. This findings indicate us that there is an apparent over expression of myconcogene at the transcriptional level. On the other hand, in choriocarcinoma cell, Southern blot hybridization of eleventh oncogenes revealed no amplification and rearrangement in any of twelfth different choriocarcinoma cell lines. Northern blot analysis revealed moderate to abundant transcripts of five oncogenes.
These elevated transcription of oncogenes at the same time may play an important role in carcinogenesis.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] S.SASAKI,Y.OHTA,T.ISOZAKI,N.NOMURA,T.ARAKI,P.A.PATTILL: "STUDY ON EXPRESSION AND AMPLIFICATION OF cーONCOGENES IN CHORIOCARCINOMA CELL LINES" TUMOR & INFECTION(腫瘍と感染). 3. 181-190 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.ISOZAKI,S.SASAKI,Y.OHTA,T.ARAKI: "EXPRESSION AND AMPLIFICATION OF ONCOGENES IN FRESH HYDATIDIFORM MOLE" TUMOR & INFECTION (腫瘍と感染). 3. 385-396 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐々木 茂・磯崎 太一: "絨毛性疾患について" 日本医事新報 No.3488(平成3年3月2月号). No.3488. 37-40 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. SASAKI, Y. OHTA, T. ISOZAKI, N. NOMURA T. ARAKI, R. A. PATTILLO: "Study on expression and amplification of c-oncogenes in choriocarcinoma cell lines" Tumor & Infection. 3. 181-190 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. ISOZAKI, S. SASAKI, Y. OHTA, T. ARAKI,: "Expression & amplification of oncogenes in fresh hydatidiform mole" Tumor & Infection. 3. 385-396 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. SASAKI, T. ISOZAKI,: "Trophoblastic diseases" Nippon Iji Shinpou. No. 3488. 37-40 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.SASAKI,Y.OHTA,I.ISOZAKI,N.NOMURA T.ARAKI,R.A.PATTILLO: "STUDY ON EXPRESSION AND AMPLIFICATION OF CーONCOGENES IN CHORIOCARCINOMA CELL LINES" TUMOR & INFECTION(腫瘍と感染). 3. 181-190 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.ISOZAKI,S.SASAKI Y.OHTA,T.ARAKI: "EXPRESSION AND AMPLIFICATION OF ONCOGENS IN FRESH HYDATIDIFORM MOLE" Tumor&Lnfection(腫瘍と感染). 3. 385-396 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐々木 茂.磯崎 太一: "絨毛性疾患について" 日本医事新報. 第3488号. 37-40 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.SAKAKI,Y.OHTA,T.ISOZAKI,N.NOMURA,T.ARAKI,R.A.PATTILLO: "Study on expression and amplification of c-oncogenes in choriocarcinoma cell lines" Tumor & Infection. 3. 1-10 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.ISOZAKI,S.SASAKI,Y.OHTA,T.ARAKI: "Expression and amplification of oncogenes in fresh hydatidiform mole" Tumor & Infection. 4. (1990)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi