1990 Fiscal Year Final Research Report Summary
Study on Expression and Amplification of C-Oncogenes in Trophoblastic Diseases
| Project/Area Number |
01570942
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SASAKI Shigeru M. D. Ph. D. Nippon Medical School Obstetrics & Gynecology Associate Professor, 医学部, 助教授 (60089737)
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| Co-Investigator(Kenkyū-buntansha) |
野村 信夫 日本医科大学老人病研究所, 助教授 (20147862)
NOMURA Nobuo Ph. D. Nippon Veterinary & Zootechnical College Molecular Oncology Associate Professor
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| Project Period (FY) |
1989 – 1990
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| Keywords | Trophoblastic disease / Hydatidiform mole / Choriocarcinoma / Oncogene / Expression / Amplification / DNA / mRNA |
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| Research Abstract |
As a first step in an ongoing study on correlation of oncogene expression and tumorigenesis in trophoblastic diseases. We determined which oncogenes were expressed in hydatidiform mole and choriocarcinoma cell lines. Southern blot hybridization and Northern blot hybridization were done with eighteenkinds of oncogene probes. No rearrangement and amplification of oncogenes were noted in fresh hydatidiform mole. But, when we checked the localization of myconcogen product at light microscopic level by avidine-biotin technique using the monoclonal antibody in the mole, strong expression of myc product was detected in cytoplasm of molar trophoblast compared with normal villi. This findings indicate us that there is an apparent over expression of myconcogene at the transcriptional level. On the other hand, in choriocarcinoma cell, Southern blot hybridization of eleventh oncogenes revealed no amplification and rearrangement in any of twelfth different choriocarcinoma cell lines. Northern blot analysis revealed moderate to abundant transcripts of five oncogenes.
These elevated transcription of oncogenes at the same time may play an important role in carcinogenesis.
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