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Interaction between brain microsomal long-chain fatty acid synthesis systems and cytoskeletons.

Research Project

Project/Area Number 01580167
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionOita Medical University

Principal Investigator

SAKAI KUMIKO (1990-1991)  Oita Medical University, Medicine, Technical Associate., 医学部, 教務職員 (60225753)

吉田 敏 (1989)  大分医科大学, 医学部, 助教授 (50158440)

Co-Investigator(Kenkyū-buntansha) YUBISUI Toshitsugu  Oita Medical University, Associate Professor, 医学部, 助教授 (00019564)
TAKESHITA Masazumi  Oita Medical University, Professor, 医学部, 教授 (50019551)
YOSHIDA Satoshi  Oita Medical University, Medicine, Associate Professor, 医学部, 助教授 (50158440)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
KeywordsLong-Chain-Fatty Acid / Cytoskeleton / Oligodendroglia / Microsomes / Fatty Acid Elongation / Condensation / Calcium / Acyl-CoA Synthetase / 脂肪酸伸長反応 / コルヒチン / サイクリックAMP / 脳ミクロソ-ム / 脂肪酸伸長 / 脂肪酸縮合 / 極長鎖脂肪酸 / パ-コ-ル法 / 細胞培養
Research Abstract

(1) The condensation activity for long-chain tatty acid was largely decreased in microsomes of isolated oligodendroslia (OLG) cells, and this decrease was caused by the change of microsomal neiibrane structure. When OLG cells were subjected to cell culture for one day with cyclic AMP analogue, condensing enzymes were distributed in microsomal membranes during culture depending on the development of cytoskeletons and also depending on enzyme species.
(2)The purified microsomal membranes were aggregated around the microtubules when ATP and GTP were included in the reaction medium, which was observed by a microscope. When microtubules were interacted with microsomal membranes, the condensation activity for the endogenous tatty acid was decreased by 30-40%, whereas that for exogenous acyl-CoA was not.
(3)The condensation activities for polyunsaturated fatty acids in rat brain microsomes were maintained high in the post-myelination period. When multiple fatty acid substrates were incubated to … More gether, the condensation activity for each tatty acid was inhibited with each other in a non-competitive manner. This inhibition manner may contribute in some part for the tatty acid homeostasis in brain.
(4)The change of membrane fluidity, or membrane disorder, by 1-5 aN calcium ion in brain microsomes was detected by Fourier transform infrared spectroscopy. Under this condition, the condensation activity for several kinds of fatty acids was increased 2- to 3-fold. This activation was caused by a change of interaction between acyl-CoA synthetase and condensing enzyme, and by a change of transfer efficiency of acyl-CoA substrates from acyl-CoA synthetase to condensins enzyme.
(5)The calcium uptake and release in microsomal membranes were largely inhibited in the presence of 18 : 4(n-3)fatty acid and malony-CoA, but not in the presence of only 18 : 4 or malonyl-CoA alone or 18 : 3(n-6)plus malonyl-CoA. ATP alone decreased the scattering of light by microsomal membrane, and under this condition the volume of microsome was increased more than twice as detected by a microscope, but without any change of condensation activity. This suggests that the elonsation system for 18 : 4(n-3)affects on the calcium mobility in microsomes, but the physiological concentration of calcium ion increased the condensation activity for polyunsaturated fatty acids with only 30 %. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Yoshida,S.and Bourre,J.M.: "Condensation activity for polyunsaturated fatty acids with malonyl-CoA in rat brain microsomes.-Characteristics and developmental change.-" Biochim.Biophys.Acta. 1123. 316-325 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida,S.,Miyata,M.,Yoshida,H.and Takeshita,M.: "change of membrane fluidity in pulmonary artery of monocrotaline-injected rats detected in situ by Fourier transform infrared spectroscopy." Vibrational Spectrosc.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kugi,M.,Yoshida,S.and TAkeshita,M.: "Characteristics of fatty acid elongation system in porcine neutrophil microsomes." Biochim.Biophys.Acta. 1043. 83-90 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida,S.,Makino,N.and Takeshita,M.: "Effect of anoxia on carotid of spontanesously hypertensive rat:Studies by scanning electron microscopy and Fourier transform infrared spectroscopy." J.Clin.Biochem.Nutr.8. 41-50 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida,S.,Miki,T.,Orii,Y.,and Takeshita,M.: "Specific inhibition of redox-linked proton pump activity of cytochrome oxidase by oleate hydroperoxide and involvment of ferrocytochrome c in the catabolism of hydroperoxide." Biochem.Int.21. 33-40 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murakami,K.,Yoshida,S.,and Takeshita,M.: "Effect of fatty-acyl-CoAs on the elongation of saturated fatty acid in porcine aorta microsomes." Biochem.Int.21. 297-304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 竹下 正純,斉藤 寿仁,吉田 敏: "ペルオキシソ-ムと神経疾患(分担)長鎖脂肪酸の合成系" 宮武 正編,、科学評論社, 249 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida, S., Saitoh, T. and Takeshita, M.: "Effect of phospholiase A2 and free fatty acids on lipid-protein interactions in long-and very-long-chain fatty acyl-CoA elongation enzyme systems of brain microsomes." Biochem. Biophys. Acta. 1004. 239-244 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takeshita, M., Yoshida, S. and Yubisui, T: "Concomitant decrease in the elongation and condensation activities of very-long chain fatty acyl-CoA in gimpy mouse." Neurochem. Int.15. 497-502 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tamura, M., Yoshida, S., Tamura, T., Saitoh, T., and Takeshita, M.: "Effect of divalent cations on NADH-dependent and NADPH-dependent cytochrome b5 reduction by hepatic microsomes." Arch. Biochem. Biophys.280. 313-319 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murakami, K., Yoshida, S., and Takeshita, M.: "Effect of fatty-acyl-CoAs on the elongation of saturated fatty acid in porcine aorta microsomes." Biochem. Int.21. 297-304 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida, S., Miki, T., Orii, Y., and Takeshita, M.: "Specific inhibition of redox-linked proton pump activity of cytochrome oxidase by oleate hydroperoxide and involvement of ferrocytochrome c in the catabolism of hydroperoxide." Biochem. Int.21. 33-40 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida, S., Makino, N. and Takeshita, M.: "Effect of anoxia on carotid of spontaneously hypertensive rat : Studies by scanning electron microscopy and Fourier transform infrared spectroscopy." J. Clin. Biochem. Nutr.8. 41-50 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kugi, M., Yoshida, S. and Takeshita, M.: "Characteristics of fatty acid elongation system in porcine neutrophil microsomes." Biochem. Biophys. Acta. 1043. 83-90 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida, S. and Bourre, J. M.: "Condensation activity for polyunsaturated fatty acids with malonyl-CoA in rat brain microsomes. -characteristics and developmental change-" Biochem. Biophys. Acta. 1123. 316-325 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida, S., Miyata, M., Yoshida, H. and Takeshita, M.: "Change of membrane fluidity in pulmonary artery of monocrotaline-injected rats detected in situ by Fourier transform infrared spectroscopy." Vibrational Spectrosc. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshida,S.and Bourre,J.M.: "Condensation activity for polyunsaturated fatty acids with malonylーCoA in rat brain microsomes.ーCharacteristics and developmental change.ー" Biochim.Biophys.Acta. 1123. 316-325 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshida,S.,Miyata,M.,Yoshida,H.and Takeshita,M.: "Change of membrane fluidity in pulmonary of monocrotalineーinjected rats detected in situ by Fourier transform infrared spectroscopy." Vibratinal Spectrosc.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kugi,M.,Yoshida,S.and Takeshita,M.: "Characteristics of fatty acid elongation system in porcine neutrophil microsomes." Biochim.Biophys.Acta. 1043. 83-90 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshida,S.,Makino,N.and Takeshita,M.: "Effect of anoxia on carotid of spontanesously hypertensive rat:Studies by scanning electron microscopy and Fourier transform infrared spectroscopy." J.Clin.Biochem.Nutr.8. 41-50 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshida,S.,Miki,T.,Orii,Y.,and Takeshita,M.: "Specific inhibition of redoxーlinked proton pump activity of cytochrome oxidase by oleate hydroperoxide and involvment of ferrocytochrome c in the catabolism of hydroperoxide." Biochem.Int.21. 33-40 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Murakami,K.,Yoshida,S.,and Takeshita,M.: "Effect of fattyーacylーCoAs on the elongation of saturated fatty acid in porcine aorta microsomes." Biochem.Int.21. 297-304 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 竹下 正純,斉藤 寿仁,吉田 敏: "ペルオキシソ-ムと神経疾患(分担)長鎖脂肪酸の合成系" 宮武正編、科学評論社, 249 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kugi Mari: "Characterization of Fatty Acid Elongation System in Porcine Neutrophil Microsomes" Biochimica et Biophysica Acta. 1043. 83-90 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida Satoshi: "Effect of Anoxia on Carotid of Spontaneously Hypertensive RatーーーStudies by Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy" Journal of Clinical Biochemistry and Nutrition. 8. 41-50 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida Satoshi: "Specific Inhibition of RedoxーLinked Proton Pump Activity of Cytochrome Oxidase by oleate Hydroperoxide and Involvement of Ferrocytochrome C in the Catabolism of Hydroperoxide" Biochemistry International. 21. 33-40 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Murakami Kazunari: "Effect of FattyーacylーCoAs on the Elongation of Saturated Fatty Acid in Porcine Aorta Microsomes" Biochemistry International. 21. 297-304 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida Satoshi: "Condensation Activity for GammaーLinolenic Acid With MalonylーCoA in Rat Brain Microsomes During Development." Lipids. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida Satoshi: "Condensation Activity for Longー and VeryーLongーChain Fatty AcylーCoAs with MalonylーCoA in Isolated Oligodendroglia." Neurochemistry International.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida,S.,Saitoh,T.and Takeshita,M.: "Effect of phospholipase A_2 and free fatty acids on lipid-protein interactions in long-and very-long-chain fatty acyl-CoA elongation systems of brain microsomes." Biochim.Biophys.Acta. 1004. 239-244 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kugi,M.,Yoshida,S.and TAkeshita,M.: "Characterization of fatty acid elongation system in porcine neutrophil microsomes." Biochim.Biophys.Acta. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshida,S.,Miki,T.Orii,Y.and Takeshita,M.: "Specific inhibition of redox-linked proton pump activity of cytochrome oxidase by oleate hydroperoxide and involvement of ferrocytochrome c in the catabolism of hydroperoxide." Biochem.International. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshida,S.,Makino,N.and Takeshita,M.: "Effect of anoxia on carotid of spontaneously hypertensive rat:Studies by scanning electron microscopy and Fourier transform infrared spectroscopy." J.Clin.Biochem.Nutr.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshida,S.,Saitoh,T.Kugi,M.and Takeshita,M.: "Characteristics of C26 fatty acid synthesis from C20 acyl-CoA in swine cerebral microsomes." Arch.Biochem.Biophys.

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshida,S.,Watari,H.Kawato,S.and Takeshita,M.: "Effect of temperature and calcium ion on long-chain acyl-CoA condensation activity and membrane dynamics in swine cerebral microsomes." J.Biol.Chem.

    • Related Report
      1989 Annual Research Report
  • [Publications] 竹下正純、斉藤寿仁、吉田敏: "ペルオキシソ-ムと神経疾患(新潟神経内科シンポジュウム シリ-ズNo.7)" 科学評論社, 249 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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