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Quantum Theory and Spectroscopy of Proteins as Biomachines

Research Project

Project/Area Number 02305013
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionOkazaki National Research Institutes, Institute for Molecular Science

Principal Investigator

KITAGAWA Teizo  Okazaki National Research Institutes / Professor, 分子科学研究所, 教授 (40029955)

Co-Investigator(Kenkyū-buntansha) MIKI Kunio  Tokyo Institute of Technology Institute for Resources and Chemistry Associate Pr, 資源化学研究所, 助教授 (10116105)
KASHIWAGI Hiroshi  Kyushu Institute of Technology Faculty of Information Engineering Professor, 情報工学部, 教授 (10000853)
HATANO Masahiro  Tohoku University Institute for Chemical Reactions Professor, 非水溶液化学研究所, 教授 (50006293)
AKUTSU Hideo  Yokohama National University Faculty of Engineering Professor, 工学部, 教授 (60029965)
MORISHIMA Isao  Kyoto University Faculty of Engineering Professor, 工学部, 教授 (50026093)
垣谷 俊昭  名古屋大学, 理学部, 助教授 (90027350)
今村 詮  広島大学, 理学部, 教授 (70076991)
郷 信広  京都大学, 理学部, 教授 (50011549)
西本 吉助  大阪市立大学, 理学部, 教授 (20046949)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1991: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1990: ¥7,600,000 (Direct Cost: ¥7,600,000)
KeywordsBiomachine / Proteins / Spectroscopy / MO calculation / Protein dynamics / 量子生物学 / 生体分子分光学 / 時間分解分光学
Research Abstract

This cooperative research aims cooperation of theoreticians in basic chemistry and biophysics with experimentalists in biochemistry and spectroscopy. The two kinds of scientists do not see each other in ordinary scientific meetings. In order to create a new concept about proteins which work as biomachines, it is desirable to prepare opportunities for the two kinds of scientists to discuss. Accordingly, we had three meetings during two years. The main subjects are electron and energy transfer in proteins, ab inito MO calculations for large molecules, time-resolved spectroscopy of biological molecules, and dynamics of proteins. Materials treated by the members include various heme proteins, photosynthetic reaction centers, retinoid proteins, various membrane proteins and enzymes. Practical activities and results are published in a separate booklet.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] S.Hashimoto,Y.Mizutani,Y.Tatsuno,T.Kitagawa: "Resonance Raman characterization of ferric-and ferrylporphyrin π cation radicals and the Fe^<IV>=O stretching frequency" J.Am.Chem.Soc.113. 6742-6549 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Egawa,T.Ogura,R.Makino,Y.Ishimura,T.Kitagawa: "Observation of the O-O stretching Raman band for cytochrome P-450_<cam> under catalytic conditions" J.Biol.Chem.266. 10246-10248 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Natai,Y.Yoneyama,T.Kitagawa: "Unusual CO binding geometry in abnormal subunits of hemoglobin M Boston and Hb M Saskatoon" Biochemistry. 30. 6495-6503 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Mizutani,S.Tokitomi,K.Aoyagi,K.Horitsu,T.Kitagawa: "Resonance Raman study on intact pea phytochrome and its model compounds;Evidence for proton migration during the phototransformation" Biochemistry. 30. 10693-10700 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Ogura,S.Takahashi,K.Shinzawa-Itoh,S.Yoshikawa,T.Kitagawa: "Time-resolved resonance Raman investigation of cytochrome oxidase catalysis:Observation of a new oxygen-isotope sensitive Raman band" Bull.Chem.Soc.Jpn.64. 2901-2907 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Takahashi,T.Ogura,K.Itoh-Shinzawa,S.Yoshikawa,T.Kitagawa: "Observation of the Cu_A-ligand stretching resonance Raman band for cytochrome c oxidase" J.Am.Chem.Soc.113. 9400-9401 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北川 禎三: "ラマン分光,第4版 実験化学講座 6" 372-388 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北川 禎三,紙中 庄司: "紫外共鳴ラマン分光による蛋白質の構造研究,分光研究 40" 193-206 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Sato, M. Asano-Someda, T. Kitagawa: "Time-resolved resonance Raman spectra of free-base octaethylporphyin in the S_1 and T_1 states" Chem. Phys. Lett.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Kaminaka, T. Kitagawa: "Time-resolved UV resonance Raman study on Bohr effects of hemoglobin" J. Am. Chem. Soc.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Kitajima, K. Fujiwara, C. Fujimoto, Y. Moro-oka, S. Hashimoto, T. Kitagawa, K. Toriumi, K. Tatsumi, A. Nakamura: "A new model for dioxygen binding in hemocyanin. Synthesis, characterization, and molecular structure of the mu-eta^2 : eta^2 peroxo dinuclear copper (II) complexes, [Cu(HB(3, 5-R_2pz)_3)]_2(O_2) (R=iPr and Ph)" J. Am. Chem. Soc.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Takahashi, T. Ogura, K. Itoh-Shinzawa, S. Yoshikawa, T. Kitagawa: "Observation of the Cu_A-ligand stretching resonance Raman band for cytochrome c oxidase" J. Am. Chem. Soc.113. 9400-9401 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Kitajima, T. Koda, S. Hashimoto, T. Kitagawa, Y. Moro-oka: "Synthesis and characterization of the dinuclear copper (II) complexes [Cu(HB(3, 5-Me_2pz)_3)]_2X (X=O^<2->, (OH)_2^<2->, CO_3^<2->, O_2^<2->)" J. Am. Chem. Soc.113. 5664-5671 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Ogura, S. Takahashi, K. Shinzawa-Itoh, S. Yoshikawa, T. Kitagawa: "Time-resolved resonance Raman investigation of cytochrome oxidase catalysis : Observation of a new oxygen-isotope sensitive Raman band" Bull. Chem. Soc. Jpn.64. 2901-2907 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Imai, K. Fushitani, G. Miyazaki, K. Ishimori, T. Kitagawa, Y. Wada, H. Morimoto, I. Morishima, D. T. -b Shih, J. Tame: "Site-directed mutagenesis in haemoglobin. Functional role of tyrosine-42 (C7) alpha at the alpha1-beta2 interface" J. Mol. Biol.218. 769-778 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Mizutani, S. Tokutomi, K. Aoyagi, K. Horitsu, T. Kitagawa: "Resonance Raman study on intact pea phytochrome and its model compounds ; Evidence for proton migration during the phototransformation" Biochemistry. 30. 10693-10700 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Nagai, Y. Yoneyama, T. Kitagawa: "Unusual CO binding geometry in abnormal subunits of hemoglobin M Boston and Hb M Saskatoon" Biochemistry. 30. 6495-6503 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Egawa, T. Ogura, R. Makino, Y. Ishimura, T. Kitagawa: "Observation of the O-O stretching Raman band for cytochrome P-450_<cam> under catalytic conditions" J. Biol. Chem.266. 10246-10248 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Kamogawa, T. Kitagawa: "Evidence for direct intermolecular interactions as an origin of the hydration shifts of the C-H stretching vibrations ; 1, 4-dioxane/water system" Chem. Phys. Lett.179. 271-276 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] V. Fidler, T. Ogura, S. Sato, K. Aoyagi, T. Kitagawa: "Resonance Raman study on photoreduction of iron-porphyrins : a novel insigh into the ligandaided process" Bull. Chem. Soc. Jpn.64. 2315-2322 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Hashimoto, Y. Mizutani, Y. Tatsuno, T. Kitagawa: "Resonance Raman characterization of ferric-and ferrylporphyrin pi cation radicals and the Fe^<IV>=O stretching frequency" J. Am. Chem. Soc.113. 6542-6549 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Mizutani, K. Kamogawa, T. Kitagawa, A. Shimizu, Y. Taniguchi, K. Nakanishi: "Solution structures and phase separation in fluoroalcohol/water mixtures ; A study with Raman difference and ^<13>C NMR spectroscopy" J. Phys. Chem.95. 1790-1794 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Nakagawa, A. Maeda, T. Ogura, T. Kitagawa: "Identification of the long-lived L' species of bacteriorhodopsin to the N intermediate by Raman/absorption quasi-simultaneous measurements" J. Mol. Struct.242. 221-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Ogura, S. Takahashi, K. Shinzawa, S. Yoshikawa, T. Kitagawa: "Observation of the Fe^<II>-O_2 stretching Raman band for compound A of cytochrome oxidase at room temperature" J. Am. Chem. Soc.112. 5630-5631 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Mizutani, S. Hashimoto, Y. Tatsuno, T. Kitagawa: "Resonance Raman pursuit of the change from Fe^<II>-O_2 to Fe^<III>-OH via Fe^<IV>=O in autoxidation of ferrous iron-porphyrin" J. Am. Chem. Soc.112. 6809-6814 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Tokutomi, Y. Mizutani, E. Anni, T. Kitagawa: "Resonance Raman scattering from large pea phytochrome at ambient temperature : Difference in chromophore protonation between red-and far-red light absorbing forms" FEBS Lett.269. 341-344 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Kitajima, H. Fukui, Y. Moro-oka, Y. Mizutani, T. Kitagawa: "A synthetic model for dioxygen binding site of non-heme proteins. X-ray structure of Fe (OBz) (MeCN) [HB(3, 5-iPr_2pz)_3] and resonance Raman evidence for reversible formation of peroxo adduct" J. Am. Chem. Soc.112. 6402-6403 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Egawa, Y. Imai, T. Ogura, T. Kitagawa: "Resonance Raman study on mutant cytochrome P-450 obtained by site directed mutagenesis" Biochim. Biophys. Acta. 1040. 211-216 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Ogura, V. Fidler, Y. Ozaki, T. Kitagawa: "Alcohol catalyzed photoreduction of iron porphyrin complexes revealed by resonance Raman and absorption spectroscopies" Chem. Phys. Lett.169. 457-459 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Kamogawa, T. Kitagawa: "A new device for Raman difference spectroscopy and its application to observe frequency shifts due to isotope mixing" J. Phys. Chem.94. 3916-3921 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Kaminaka, T. Ogura, T. Kitagawa: "Time-resolved ultraviolet resonance Raman study of the photolysis of carbonmonoxy hemoglobin : Relaxation of the globin structure" J. Am. Chem. Soc.112. 23-27 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Hashimoto,Y.Mizutani,Y.Tatsuno,T.Kitagawa: "Resonance Raman characterization of ferric^ー and ferrylporphyrin π cation radicals and the Fe^<IV>=0 stretching frequency" J.Am.Chem.Soc.113. 6542-6549 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Egawa,T.Ogura,R.Makino,Y.Ishimura,T.Kitagawa: "Observation of the 0ー0 stretching Raman band for cytochrome Pー450_<cam> under catalytic conditions" J.Biol.Chem.266. 10246-10248 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Nagai,Y.Yoneyama,T.Kitagawa: "Unusual CO binding geometry in abnormal subunits of hemoglobin M Boston and Hb M Saskatoon" Biochemistry. 30. 6495-6503 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Mizutani,S.Tokutomi,K.Aoyagi,K.Horitsu,T.Kitagawa: "Resonance Raman study on intact pea phytochrome and its model compounds;Evidence for proton migration during the phototransformation" Biochemistry. 30. 10693-10700 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Ogura,S.Takahashi,K.ShinzawaーItoh,S.Yoshikawa,T.Kitagawa: "Timeーresolved resonance Raman investigation of cytochrome oxidase catalysis:Observation of a new oxygenーisotope sensitive Raman band" Bull.Chem.Soc.Jpn.64. 2901-2907 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Takahashi,T.Ogura,K.ItohーShinzawa,S.Yoshikawa,T.Kitagawa: "Observation of the Cu_Aーligand stretching resonance Raman band for cytochrome c oxidase" J.Am.Chem.Soc.113. 9400-9401 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北川 禎三: "ラマン分光,第4版 実験化学講座 6" 372-388 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北川 禎三,紙中 庄司: "紫外共鳴ラマン分光による蛋白質の構造研究,分光研究 40" 193-206 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Kaminaka;T.Ogura;T.Kitagawa: "Timeーresolved ultraviolet resonance Raman study of the photolysis of carbonmonoxy hemoglobin: Relaxation of the globin structure." Journal of American Chemical Society,<112>___ー,23ー27 (1990). 112. 23-27 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Ogura;V.Fidler;Y.Ozaki;T.Kitagawa: "Alcohol catalyzed photoreduction of iron porphyrin complexes revealed by resonance Raman and absorption spectroscopies." Chemical Physics Letters. 169. 457-459 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Egawa;Y.Imai;T.Ogura;T.Kitagawa: "Resonance Raman study on mutant cytochrome Pー450 obtained by site directed mutagenesis." Biochimica Biophysica Acta. 1040. 211-216 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Ogura;T.Kitagawa;S.Takahashi;K.Shinzawa;S.Yoshikawa: "Observation of the Fe^<II>ーO_2 stretching Raman band for compound A of cytochrome oxidase at room temperature." Journal of American Chemical Society. 112. 5630-5631 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Mizutani;S.Hashimoto;Y.Tatsuno;T.Kitagawa: "Resonance Raman pursuit of the change from Fe^<II>ーO_2 to Fe^<III>ーOH via Fe^<IV>=O in autoxidation of ferrous ironーporphyrin." Journal of American Chemical Society. 112. 6809-6814 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Ogura;T.Kitagawa;S.Takahashi;K.Shinzawa;S.Yoshikawa: "Observation of the Fe^<4+>+O stretching Raman band for cytochrome oxidase compound B at ambient temperature." Journal of Biological Chemistry. 265. 14721-14723 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Imai;T.Kitagawa;D.Shie;K.Ishimori;Y.Wada;J.Tame;K.Fushitani;H.Morimoto;K.Nagai;G.Miyazaki;I.Morishima: "Protein Structural Analysis,Folding and Design" Japan Science Society Press, 230 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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