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Genetic analysis of a closed herd of Japanese Black cattle in Tajima district using DNA polymorphisms

Research Project

Project/Area Number 02454093
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionKobe University

Principal Investigator

GOTO Nobuo  Kobe Univ., Faculty of Agr., Professor, 農学部, 教授 (80195940)

Co-Investigator(Kenkyū-buntansha) MUKAI Fumio  Kobe Univ., Faculty of Agr., Research assistant, 農学部, 助手 (50093323)
TSUJI Soichi  Kobe Univ., Faculty of Agr., Associate professor, 農学部, 助教授 (10031220)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsDNA fingerprint / Japanese Black cattle / parentage identification / RFLP / bovine lipoprotein lipase cDNA / 但馬牛 / 個体識別
Research Abstract

To utilize a closed herd of 'Tajima cattle' having high quality beef for genetic improvement of Japanese Black cattle effectively, genetic constitution of the herd was investigated by using DNA polymorphism analysis. The analysis was also applied to parentage identification.
1. DNA fingerprinting was examined with a M13 phage DNA probe in the cattle of 42 families extracted from the herd. The parentage of each family was identified by using some restriction endonucleases such as Hae III.
2. A linear regression of Genetic Similarity level(X) estimated from DNA fingerprints on relationship coefficient(Y) yielded the equation, Y = 1.149X - 0.026, with highly significant correlation coefficient,indicating that DNA fingerprinting could be applied to estimate the relation coefficient in cattle.
3. Cluster analysis based on banding patterns in DNA fingerprints showed that the herd is roughly divided into two groups. This result depended mainly on the frequencies of two bands(minisatellite loci).
4. Using bovine cDNA of lipoprotein lypase as a probe, restriction fragment length polymorphisms(RFLP) have been examined in the herd. The 1.3kb probe(5'-half containing open reading frame) gave distinctive RFLPs with 5 restriction enzymes such as Pst I. Types of RFLP being given with each restriction enzyme were closely related to each other and expressed as haplotypes A and B, which suggests all RFLP types are linking. It was shown that Aand B RFLP types are codominantly inherited. Heifers of 116 Japanese Black of the herd were classified into 3 groups, AA(15 heads), AB(65) and BB(35), with the gene frequencies of 0.418 (haplotype A) and 0.582 (B).

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Fumio Mukai: "Body size and reproductive performance traits of a closed herd of Japanese Black cattle" Journal of Animal Breeding and Genetics. 107. 282-290 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 万年 英之: "家畜におけるDNAフィンガープリント法" 動物蛋白質血液型多型研究会報. 19. 10-17 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 万年 英之: "M13ファージ反復配列をプローブとしたウシのDNAフィンガープリント分析" 日本畜産学会報. 63. 928-934 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 向井 文雄: "黒毛和種閉鎖育種集団における繁殖雌牛の体測定値の遺伝的趨勢" 日本畜産学会報. 63. 398-406 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 向井 文雄: "性により記録される形質が異なる場合のアニマルモデルによる育種価評価値の正確度" 日本畜産学会報. 63. 488-494 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 向井 文雄: "アニマルモデルBLUP法による枝肉形質に関する育種価予測値の正確度の近似値" 日本畜産学会報. 64. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hideyuki Mannen: "Application of DNA fingerprinting to domestic animals" Animal Blood Research Information. 19. 11-18 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fumio Mukai: "Body size and reproductive performance traits of a closed herd of Japanese Black cattle" Journal of Animal Breeding and Genetics. 107. 282-290 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fumio Mukai: "Genetic trends of body measurements of cows in a closed Japanese Black cattle population" Animal Science and Technology. 63. 398-406 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fumio Mukai: "Accuracy of predicted breeding values by animal models when main and secondary traits are recorded in different sex" Animal Science and Technology. 63. 488-494 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hideyuki Mannen: "DNA fingerprint analysis of cattle using bacteriophage M13 repetitive sequence as a probe" Animal Science and Technology. 63. 928-934 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Fumio Mukai: "Approximate accuracies of predicted breeding values for carcass traits under an animal model" Animal Science and Technology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 万年 英之: "M13ファージ反復配列をプローブとしたウシのDNAフィンガープリント分析" 日本畜産学会報. 63. 928-934 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 向井 文雄: "黒毛和種閉鎖育種集団における繁殖雌牛の体測定値の遺伝的趨勢" 日本畜産学会報. 63. 398-406 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 向井 文雄: "性により記録される形質が異なり場合のアニマルモデルによる育種価評価値の正確度" 日本畜産学会報. 63. 488-494 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 向井 文雄: "アニマルモデルBLUP法による枝肉形質に関する育種価予測値の正確度の近似値" 日本畜産学会報. 64. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 万年 英之: "M13ファ-ジ反復配列をプロ-ブとしたウシのDNAフィンガ-プリント分析" 日本畜産学会報.

    • Related Report
      1991 Annual Research Report
  • [Publications] Hideyuki Mannen: "Relationship between relationship coefficient and genetic similarity using DNA fingerprinting in cattle" Journal of Heredity.

    • Related Report
      1991 Annual Research Report
  • [Publications] Fumio Mukai: "History and population structure of a closed strain of Japanese Black Cattle" Journal of Animal Breeding and Genetics. 106. 254-264 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fumio Mukai: "Body size and reproductive performance traits of a closed herd of Japanese Black Cattle" Journal of Animal Breeding and Genetics. 107. 282-290 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 万年 英之: "家畜におけるDNAフィンガ-プリント法" Anim.Blood Res.Inform.19. 10-17 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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