THE PATHOGENETICAL ROLE OF VIRUS INFECTION AND ACTIVATION OF ONCOGENES.
Project/Area Number |
02454220
|
Research Category |
Grant-in-Aid for General Scientific Research (B)
|
Allocation Type | Single-year Grants |
Research Field |
内科学一般
|
Research Institution | Fukui Medical School |
Principal Investigator |
HOSHINO Takashi Fukui Medical School, Department of Immunology and Parasitology, Professor, 医学部, 教授 (50026853)
|
Project Period (FY) |
1990 – 1992
|
Project Status |
Completed (Fiscal Year 1992)
|
Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥2,700,000 (Direct Cost: ¥2,700,000)
|
Keywords | SLE / EB virus / EBNA subtypes / C-myc product / C-fos product / HL-60 cell / Transcript activating factor / Production of autoantibody / EBNA subtypes / 膠原病 / 癌遺伝子 / 抗癌遺伝子蛋白抗体 / 抗cーmyc抗体 / 抗cーfos抗体 / EBV感染B細胞 / HLー60分化誘導 / 全身性自己免疫疾患 / RA / EBウイルス核抗原(EBNA) / 抗EBNAサブクラス抗体 |
Research Abstract |
In order to clarify the role of virus infection and subsequent activation of protooncogenes on the triggering process of collagen disease,, the sera from 66 patients with SLE were analyzed by the immunoblotting method to detect antibodies to the antigens on the EB virus genome positive Raji and P3HR-1 cells, as well as on the cultured myeloid cell lines of various stages of differentiation. The sera from SLE patients were found to contain the antibodies to the antigens with Mr of 66K, 70K, 90K, 140K and 160K daltons on Raji cells which were identified as c-myc protein and EB virus nuclear antigen (EBNA) subtypes 1, 2, 3 and 4, respectively. The frequency and amount of antibodies against EBNA-2 and -3 were significantly higher than in 60 control normal sera. Further, the sera from SLE patient were found to have the antibodies to the antigens with Mr of 60K on K-562, KG-1 and HL-60 cells, which are also known to be c-myc product. After an incubation of HL-60 cells with TPA or vitamin D3 to induce their macrophage differentiation, the SLE sera became to detect the 55K and 39K antigens on the differentiated HL-60 cells, while the 60K antigen turned to undetectable or only faintly detected. Polyclonal antibodies to myc-specific and fos-specific peptides were applied to react with these antigens including cross experiments. The results of these studies confirmed that the sera from SLE patients contain antibodies to c-myc, c-fos and possibly c-Jun/AP-1 protooncogen products. In addition, SLE patients were found to show EBNA positive B cells at 3 times higher frequencies than that of B cells from normal subjects. It was suggested that EB virus infection with subsequent activation of several oncogenes may have an important pathogenetical role on the triggering process of SLE.
|
Report
(4 results)
Research Products
(13 results)