| Project/Area Number |
02670334
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
MOTOMIYA Maskichi Res. Inst. Tb. & Cancer, Tohoku Univ. Professor, 抗酸菌病研究所, 教授 (20006092)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Tatsuya Res. Inst. Tb. & Cancer, Tohoku Univ. Res. Associate, 抗酸菌病研究所, 助手 (70222651)
SATOH Ken Res. Inst. Tb. & Cancer, Tohoku Univ. Res. Associate, 抗酸菌病研究所, 助手 (00215782)
永井 宏美 東北大学抗酸菌病研究所, 助手 (60217958)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Lung Cancer / Fibrosis / Fibronectin / Laminin / Receptor / Molecular Biology / ラミニンレセプタ- / 肺腺維症 / フィブロネクチン / ラミニンリセプタ- / 免疫組織染色 / 肺胞マクロファ-ジ |
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| Research Abstract |
1. A high incidence of lung cancer accompanied with pulmonary fibrosis was demonstrated. Cell types of complicated lung cancer were mainly squamous cell carcinoma and differentiated adenocarcinoma.
2. Biochemical analyses of adhesion proteins such as fibronectin and laminin were carried out in the tissues of pulmonary fibrosis and lung cancer. As a result, increased amount of fibronectin with low molecular weight was recognized in pulmonary figrosis. On the other hand, fibronectin with low affinity to heparin was increased in lung cancer. There was no quantitative and qualitative difference in tissue laminin between pulmonary fibrosis and lung cancer.
3. In addition to fibronectin and laminin, it is also necessary to analyze their receptors on the cells associating with pulmonary fibrosis and lung cancer. For that purpose, antibody for the 67kDa-laminin receptor was obtained. Also cDNA for the receptor was cloned from human small cell lung cancer. The experiments using the antibody and cDNA showed that the expression of 67kDa-laminin receptor was increased in undifferentiated lung cancer cell which was rarely accompanied with pulmonary fibrosis.
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