HPLC Separation of Metal Chelates Utilizing Adduct Formation

Research Project

Project/Area Number	03640486
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	分析・地球化学
Research Institution	Faculty of Engineering, Gunma University
Principal Investigator	TSUNODA Kin-ichi Fac. of Eng., Gunma Univ., Assoc. Prof., 工学部, 助教授 (30175468)
Co-Investigator(Kenkyū-buntansha)	KAWAMOTO Hiroshi Fac. of Eng., Gunma Univ., Prof., 工学部, 教授 (20008460) AKAIWA Hideo Fac. of Eng., Gunma Univ., Prof., 工学部, 教授 (60008414)
Project Period (FY)	1991 – 1992
Project Status	Completed (Fiscal Year 1992)
Budget Amount *help	¥2,000,000 (Direct Cost: ¥2,000,000) Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords	Adduct / HPLC / Metals / 4-(2-thienyl)-4-thioxo-1,1,1-trifluoro-2-butanone / 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione / 金属キレ-ト
Research Abstract	The separation behavior of Co^<III>-, Ni^<II>-, Cu^<II>-, and Zn^<II>-STTAs [4-(2-thieny1)-4-thioxo-1,1,1-trifluoro-2-butanone] and Co^<II>-, Ni^<II>-, and Cu^<II>-TTAs [4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione] in reversed phase high performance liquid chromatography was investigated when various additives were added to the mobile phase. The adduct formation or the masking effect was observed upon addition of additives. When trioctylphosphine oxide was added to the mobile phase, mutual separation of those metal chelates was especially remarkable due to the adduct formation. Moreover, the decomposition of some metal chelates in the HPLC column was also prevented by the adduct formation. On the other hand, 2,2'-bipyridyl and 1,10-phenanthroline showed the masking effect. Such effects of additives were consistent with their synergistic extraction constants in chelate extraction.