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Physiological Research of Photoperiodic nesporse of insect using laser micro-beam.

Research Project

Project/Area Number 03640602
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SHIMIZU Isamu  Kyoto Univ.Center for Elological Research, associate Professor, 生態学研究センター, 助教授 (80025486)

Project Period (FY) 1991 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordslaser-microbeam / Insect / Silkworm / diapause / diapause-hormone / neuro-sencation / phortoperiodism / 昆虫ホルモン / レ-ザ-メス
Research Abstract

An apparatus which is devised to illuminate ultraviolet laser- microbeam to insect tissue and organ, and to examine its effect on the photoperiodic response of insects, was constructed. At first I tried an illumination of the laser-microbeam to subeophageal ganglion(SG) of the silkworm, in which the hormonal effector system is located. Brain(Br)-SG complex was extirpated from the head of pupae of diapause-egg producers, and setted on a glass- plate. The damage of the tissue was confirmed by an observation of some small bubbles which produced on the surface of the ganglions. When the beam was illuminated on the location of neurosecretory cells in SG the diapause percentage became 0%, while the control without illumination was 100%, it means that this laser-treatment was very effective as a micro-treatment technique. Regarding the illumination to brain of the silkworm, only preliminary results were obtained, but the destory of the some parts of protocerebral ganglion suggests that a central regulatory site might be located in this area.

Report

(3 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] AOKI S.: "Mechamism of syntkesis and secreation of diaqaese hormere in the silkwom,Bambyx mori" Zoological Science. 8. 1182-1183 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKAMOTO K.: "Effect of carotenaid deficimay in circadion rhythm in gatding of the silkwom Bambyx mori" Zoological Science. 8. 1037-1038 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 清水 勇: "カイコの化性と光周性反応" 応用動物昆虫学雑誌. 35. 83-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 清水 勇: "カイコ幼虫の走光性行動" 日蚕雑. 60. 91-96 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SHIMIZU.I.: "Comqarison of fatty and comqorition in liqids of diaqanse and xm-diaqasees of Bomayx mori" Comq.Biochem.Physiol. 102B. 713-716 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SHIMIZU.I.: "Regalation of diaqanse karmore secretion in the siekworm,Bomibymori:using laser treatment" Zoological Science. 9. 1271 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shimizu I.: "Regulation of diapause hormone senetion in the Silkworm,Bombyxmori:using laser microbeam treatment." Zoological Science. 9. 1271 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aoki S.: "Mechanism of synthesis and secreation of diapause hormane in the Silkworrn,Bombyx mori." Zoological Science. 8. 1182-1183 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakamoto K.: "Effect of carotenaid detiaimay an circadiam rhythm in latding of the silicwom.Bamvyx mori" Zoological Science. 8. 1037-1038 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 清水 勇: "カイコの化性と光周性反応" 応用動物昆虫学雑誌. 35. 83-91 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 清水 勇: "カイコ幼虫の走光性行動" 日蚕雑. 60. 91-96 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimizu.I.: "Comparison of tatly and comporition in lipids of diapanse and non-diapance eggs of Bombyx mori" Comp.Biochem.Physiol.102B. 713-716 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shimizu.I.: "Regulation of diapanse hormone secretion in the silkwornm,Bonbymori:using laser treat." Zoological Science. 9. 1271- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 清水 勇: "カイコの化性と光周性反応" 応用動物昆虫学雑誌. 35. 83-91 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Aoki,S: "Mechauism of Symtheris and secretion of cliapauce hormone in the silk worn,Bombyxmori" Zoolosical Science. 8. 1181 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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