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Evaluation of blood protein and cell adsorption on plasma filters

Research Project

Project/Area Number 03670761
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionTOKAI UNIV.SCHOOL OF MEDICINE

Principal Investigator

SATOH Takeshi  TOKAI UNIV.SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (20055792)

Co-Investigator(Kenkyū-buntansha) KITAMURA Makoto  TOKAI UNIV.SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (00214817)
HIRAGA Seigo  TOKAI UNIV.SCHOOL OF MEDICINE, ASS.PROFESSOR, 医学部, 助教授 (10114779)
HIDA Miho  TOKAI UNIV.SCHOOL OF MEDICINE, ASS.PROFESSOR, 医学部, 助教授 (20147169)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
KeywordsPlasma filter / membrane adsorption / electron microscopy / SDS-PAGE / Hydrophilic membrane / Nonhydrophilic membrane / 蛋白・血球の付着 / ヘパリン / メシル酸ナファモスタット / 血球・蛋白付着 / 電子顕微鏡写真 / SDS電気泳動
Research Abstract

Membrane materials interact differently with blood. In the present report, adsorption of blood components(proteins and cells) on hydrophilic polyvinyl alcohol (PVA) and nonhydrophilic polysulfon (PS) filters were investigated. Proteins and cells were detected by scanning electron microscopy and the amounts of adsorbed proteins were analyzed by SDS-PAGE methods. The following results were obtained.
1, Blood components were slightly adsorbed on the PVA filter. The structure of the inner wall of the filter was well preserved. On the other hand, large quantities of blood components were seen on the PS filter, and the inner wall structure was barely recognizable.
2, When the amount of adsorbed albumin on the PVA filter was fixed at 1.0, the amount of adsorbed proteins on this filter was calculated to be 2.0-2.5, the sum of proteins such as albumin (1.0), IgG (0.6-0.8), fibrin (0.1-0.2) and erythrocytic proteins (0.5-0.7). The amount of adsorbed protein on the PS filter, calculated to be 4.0-4.5, was the sum of proteins such as albumin (1.2-1.5), IgG (0.8-1.1), fibrin (0.4-0.5) and erythrocytic proteins (0.9-1.1). In conclusion, these results may contribute to understanding the anti-thrombogenecity of PVA filters.

Report

(4 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 渡辺順 飛田美穂 佐藤威: "血漿分離膜に吸着した血液成分の検討" 日本透析療法学会雑誌. 26. 1369-1373 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Jun Watanabe, Miho Hida, Takeshi Satoh: "Evaluation of blood protein and cell adsorption on plasma filters" Journal of Japanese Society for dialysis Therapy. 26. 1369-1373 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 渡辺順: "血漿分離膜に吸着した血液成分の検討" 日本透析療法学会雑誌. 26. 1369-1374 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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