• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on correlation between intra-tumoral heterogeneity and tumor invasiveness in urological cancers.

Research Project

Project/Area Number 04404064
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionSchool of Medicine, Keio University

Principal Investigator

TAZAKI Hiroshi  Keio Univ., Dept.of Urology, Professor, 医学部, 教授 (90051268)

Co-Investigator(Kenkyū-buntansha) MIYAKAWA Harumi  Keio Univ., Dept.of Urology, Assistant, 医学部・泌尿器科, 助手 (40239356)
SAITO Shiro  Keio Univ., Dept.of Urology, Assistant, 医学部・泌尿器科, 助手 (80170504)
MARUMO Ken  Keio Univ., Dept.of Urology, Assistant Professor, 医学部・泌尿器科, 講師 (80138130)
BABA Shiro  Keio Univ., Dept.of Urology, Assistant Professor, 医学部・泌尿器科, 講師 (00051889)
TACHIBANA Masaaki  Keio Univ., Dept.of Urology, Assistant Professor, 医学部・泌尿器科, 講師 (70129526)
中島 淳  慶應義塾大学, 医学部, 助手 (10167546)
出口 修宏  慶應義塾大学, 医学部, 助教授 (90118977)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsUrological cancers / Heterogeneity / Tumor investiveness / Cell growth / Flow cytometry / 浸潤性判定
Research Abstract

In as much as cancer consists of a spectrum of diseases with diverse natural hitories, the conventional morphological classifications provided by classical histology still remain the hallmarks in the decision-making process. Intra-tumoral heterogeneity thought to be the one of principal factors for the indicator of tumor invasiveness and/or responses to various treatments. In the pesent study, an attempt was made to clarify what tumor heterogeneity provide significantly strong parameter for determination of the malignant potential and/or invasiveness of cancer. We have found followings
1. Flow cytometric deoxyribonucleic acid (DNA) /bromodeoxyuridine bivariate analysis provide important information on the malignant potential of bladder cancer. Also was found that the proliferative rate of the tumor cells in tumor may have significant importance since the rate of DNA synthesis is directly related to the rate of tumor growth or to tumor involvement.
2. p53 supressor gene mutatin can be detected in cases which revealed tumor recurrence in the bladder. Among then, primary and recurrent tumor exhibited identical point mutations of p53.
3. Biologically active proteins producing renal cell carcinomas were successfully established. Erythropoietin and granulocyte macrophage-colony stimulating factor (GM-CSF) producing cells demonstrated their rapid cell cycling and metastatic tendency by cloning.
4. Also was found tumor necrosis factor producing prostate cancer cells exhibited higher degree of biologically malignant potential.
5. Meanwhile, alpha-fetoprotein (AFP) producing testicular cancer cells revealed relatively low proliferative activity, and the AFP production can be increased by treatment of recinoic acid.
6. These results indicate that intra-tumoral heterogeneity strongly correlate with differentiation of tumor cells, and this type of differentiations such as meker protein productions can be identified as a biologically worse characteristics.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Tachibana,M.,et al.: "Bromodeoxyuridine bnd deoxyribonusleic asid bivariate analysis in human renal cell carcinoma:Does flow cytometric determination predic malignant potential or prognosis of patients with renal cell carcinoma?" American Journal of Clinical Pathology. 97. S38-S47 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakanoma,T.et al.: "Immunohistological analysis of tumor infiltrating lymphocytes in seminoma using monoclonal antibodies" Virchows Archiv A Pathol Anat.421. 409-413 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橘 政昭、他: "腎細胞癌における生理活性物質とその随伴症状する検討" KARKINOS. 5. 1141-1146 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 宮川絢子、他: "泌尿器科領域における遺伝子診断・治療の最近の動向膀" Human Cell. 5. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 田崎 寛、他: "研究室で役に立つつ細胞株-ヒト細胞癌:U-7,KU-1,NBT-2" 生体の科学. 43. 407-408 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 田崎 寛、他: "前立腺肥大症前立腺癌の鑑別診断膀胱腫瘍治療の新しい" ライフ・サイエンス. 30. 1919-1925 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 宮川絢子、他: "Anchored cell analysis sorting (ACAS)system" Human Cell. 5. 392-397 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橘 政昭、他: "Kock回腸膀胱造設における手術手技変更に伴う早期ならびに晩期合併症とその成因に関する検討" 日本泌尿器科学会雑誌. 83. 1890-1897 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橘 政昭、他: "表在性膀胱癌に体するBCG膀胱内注入療法の平均観察期間5年における再発予防効果" 日本泌尿器科学会雑誌. 84. 656-661 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tachibana,M.et al.: "Prognostic significance of bromodeoxyuridine high labeled bladder cancer measured by flow cytometry:Does flow cytometric determination predict the prognosis of patients with transitional cell carcinoma of the bladder." The Journal of Urology. 149. 739-743 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橘 政昭: "細胞診とFlow cytometry:膀胱腫瘍治療の新しい展開" 泌尿器科 Mook. 5. 51-64 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 橘 政昭: "若年者膀胱癌の病態と特徴" KARKINOS. 6. 741-745 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tachibana,M.et al.: "A new scoring system based on the histological behavior and proliferative activity of tumor cells for grading tne malignant potential of bladder cancers" International Journal of Urology. 1. 37-42 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shibayama,T.,et al.: "Disappearance of metastatic renal cell carcinoma to the base of the tongue after systemic administration of interferon-alpha." Eur.,Urol.24. 297-299 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 田崎 寛、他: "胃癌のサイトカイン療法" 癌と化学療法. 21. 29-36 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 成松芳明、他: "術後の画像診断-治療効果判定のポイント" 臨床画像. 66-78 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa,A.et al.: "Flow cytometric bromodeoxyuridine/deoxyribonucleic acid bivariate analysis for predicting tumor invasiveness of upper tract urothelial cancer" The Journal of Urology. 152. 76-80 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa,A.et al.: "Flow cytometric measurements of deoxyribonucleic acid ploidy and proloferative activity for eveluating malignant potential of human bladder cancers" Cancer Detection and Prevention. 19. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshimura,I.,et al: "p53gane mutation in recurrent superficial bladder cancer." The Journal of Urology. 153. 1-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tachibana, M., Deguchi, N., Baba, S., Jitsukawa, S., Jitsukawa, S., Hata, M.and Tazaki, H.: "Bromodeoxyuridine and deoxyribonusleic asid bivariate analysisin human renal cell carcinoma : Does flow cytometric determination predic malignant potential or prognosis of patients with renal cell carcinoma?" American Journal of Clinical Pathology. 97. s83-s47 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakanoma, T., Nakamura, K., Deguchi, N., Fujimoto, J., Tazaki, H.and Hata, J.: "Immunohistological analysis of tumor infiltrating lymphocytes in seminoma using monoclonal antibodies" Virchows Archiv A Pathol Anat.421. 409-413 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa, A., Tachibana, M.and Tazaki, H.: "Ahchored cell analysis sorting (ACAS) system" Human Cell. 5. 392-397 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tachibana, M., Deguchi, N., Baba, S., Jitsukawa, S., Hata, M.and Tazaki, H.: "Prognostic significance of bromodeoxyuridine high labeled bladder cancer measured by flow cytometry : Does flow cytometric determination predict the prognosis of patients with transitional cell carcinoma of the bladder." The Journal of Urology. 149. 739-743 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tachibana, M., Miyakawa, A., Deguchi, N., Baba, S., Murai, M.and Tazaki, H.: "A new scoring system based on the histological behavior and proliferative activity of tumor cells for grading the malignant potential of bladder cancers" International Journal of Urology. 1. 37-42 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shibayama, Hasegawa, S., T., Nakamura, S.and Tachibana, M.: "Disappearance of metastatic renal cell carcinoma to the base of the tongue after systemic administration of interferon-alpha." Eur., Urol.24. 297-299 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa, A., Tachibana, M., Nakashima, J., Deguchi, N., Baba, S.and Tazaki H.: "Flow cytometric bromodeoxyuridine/deoxyribonucleic acid bivariate analysis for predicting tumor invasiveness of upper tract urothelial cancer" The Journal of Urology. 152. 76-80 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa, A., Tachibana, M.and Tazaki, H.: "Flow cytometric measurements of deoxyribonucleic acid ploidy and proloferative activity for eveluating malignant potential of human bladder cancers" Cancer Detection and Prevention. 19. 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshimura, I., Kudoh, J., Saito, S., Tazaki, H.and Shimizu N.: "The Journal of Urology" p53 gene mutation in recurrent superficial bladder cancer.153. 1-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masaaki Tacahibana: "A new scoring system based on the histological behavior and proliferative activity of tumor cells for grading the malignent potontial of bladder cancers" Int'1 Journal of Urology. 1. 37-42 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ichiro Yoshimura: "p53 gene mutation in recurrent superficial bladder cqncer" The Journal of Urology. 153(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田崎 寛: "腎癌のサイトカイン療法" 癌と化学療法. 21. 29-36 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] TACHIBANA M,Hazaki H,et al.: "Rrognostic significance of BrdU hifh labeled bladder cancer measured by flow cytometry" The Jornal of Urology. 149. 739-743 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shibayama T,Tashibana M,et al.: "Disoppearance of metastatic renal cell carcinoma to the base of the tongul after systemic administration ofinterferon-alpha" European Urology. 24. 297-299 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 橘 政昭、田崎 寛他: "表在性膀胱癌に対するBCG膀胱内注入療法の平均観察期間5年における再発予防効果" 日本泌尿器科学会雑誌. 84. 656-661 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 橘 政昭: "若年者膀胱癌の病態と治療の特徴" KARKINOS. 6. 741-745 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Miyakawa A,Hazaki H,et al.: "Flow cytometric BrdU/DNA bivariate analysls for predicting tumor invasiveness of upper tract urothelial cancer cancer" The Journal of Urology. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Tachibane M.et al: "Bromodeoxyuridine and Deoxyribonucleic Acid Bivariate Analysis in Human Renal Cell Carcinoma" J.Clin.Pathol. 97. S58-S47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 宮川 絢子,他: "泌尿器科領域における遺伝子診断・治療の最近の動向" Human Cell. 5. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakanoma T.et al: "Immunohistlogical analysis of tumor infiltratting lymphocytes in seminoma using monoclonal antibodies" Virchows Archiv.A Pathol Anat. 421. 409-413 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 宮川 絢子,他: "Anchored Cell Analysis and Sorting(ACAS)System" Human Cell. 5. 392-397 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tachibana M. et al: "Prognostic Significance of bromodeoxyuridine high-labeled bladder cancer" J.Urol.

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi