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Predictive assay for late radiation injury with fibroblast and lymphocyte

Research Project

Project/Area Number 04670677
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionSchool of Medicine, Keio University

Principal Investigator

ITO Hisao  Keio Univ.Depart.Radiol.Associ.Prof., 医学部, 助教授 (20095574)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Toshitake  Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (90189077)
SHIGEMATSU Naoyuki  Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (30178868)
NISHIGUCHI Iku  Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (20198451)
OGAWA Koichi  Hosei Univ.Fac.of Ergeen.Associate Prof., 理工学部, 助教授 (00158817)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsRadiotherapy / Late radiation injury / Fibroblast / Lymphocyte / Predictive assay / 放射線慢性障害 / 放射線感受性 / 慢性放射線障害 / 耐用線量 / 光学的生存率測定 / 初代培養細胞
Research Abstract

Radiotherapy is very useful treatment modality for cancer patients. However, there are several problems to perform clinical radiotherapy. Those are (1) it is very difficult to prospect radiosensitivity of each cancer before radiotherapy, and (2) it is impossible to predict late radiation injury before it happens. This study was performed to develop the predictive assay method to detect late radiation damage and radio-sensitivity of cancer before radiotherapy. Patients with cervical cancer were taken the cancer tissue, the skin subcutaneous tissue and lymphocytes. Cultured cells were established from these tissues. They were irradiated and radiosensitivities for each cell strain were determined. The survival curves were made with densitometry assay method for cancer cells, and with ordinary colony assay method for fibroblasts and lymphocytes. The growth rates of fibroblast were different in each strain and it was very difficult to establish the useful assay method. For cancer cells, the densitometry assay showed slightly higher survival rates than the colony assay, but there was good correlation between these two methods. On the other hand, the relationship between radiosensitivities of fibroblasts and lymphocytes and late radiation injury was not determined. The reasons were (1) number of successful cultures was short, and (2) an incidence of late radiation injury was small number. We concluded that it is important to develop the more easy and sensitive assay method, for example, measurement of some factors in the serum. In future, the developments of molecular analyzes of radiation damage can provide the some better assay methods.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Hisao Ito, Shoji Kutuki, Iku Nishiguchi et al.: "Radiotherapy for cervical cancer with high dose rate brachy-therapy-Correlation between tumor size, dose and failure" Radiother. Oncol.31. 240-247 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hisao Ito, Rituko Komaki and Luka Milas.: "Protection by WR-2721 against radiation plus cis-diammine dichloroplatinum II caused injury to colonic epithelium in mice." Inter J Radiat Oncol Biol Phys. 28. 899-903 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hisao Ito, Shoji Kutuki, Iku Nishiguchi, et al.: "Radiotherapy for cervical cancer with high dose rate brachytherapy-Correlation between tumor size, dose and failure" Radiother, Oncol.31 (3). 240-247 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hisao Ito, Rituko Komaki, Luka Milas.: "Protection by WR-2721 against radiation plus cis-diamminedichloroplatinum II caused injury to colonic epithelium in mice." Inter J Radiat Oncol Biol Phys. 28 (4). 899-903 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary

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Published: 1992-04-01   Modified: 2016-04-21  

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