1993 Fiscal Year Final Research Report Summary
Predictive assay for late radiation injury with fibroblast and lymphocyte
| Project/Area Number |
04670677
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | School of Medicine, Keio University |
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Principal Investigator |
ITO Hisao Keio Univ.Depart.Radiol.Associ.Prof., 医学部, 助教授 (20095574)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Toshitake Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (90189077)
SHIGEMATSU Naoyuki Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (30178868)
NISHIGUCHI Iku Keio Univ.Depart.Radiol.Associate, 医学部, 助手 (20198451)
OGAWA Koichi Hosei Univ.Fac.of Ergeen.Associate Prof., 理工学部, 助教授 (00158817)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Radiotherapy / Late radiation injury / Fibroblast / Lymphocyte / Predictive assay |
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| Research Abstract |
Radiotherapy is very useful treatment modality for cancer patients. However, there are several problems to perform clinical radiotherapy. Those are (1) it is very difficult to prospect radiosensitivity of each cancer before radiotherapy, and (2) it is impossible to predict late radiation injury before it happens. This study was performed to develop the predictive assay method to detect late radiation damage and radio-sensitivity of cancer before radiotherapy. Patients with cervical cancer were taken the cancer tissue, the skin subcutaneous tissue and lymphocytes. Cultured cells were established from these tissues. They were irradiated and radiosensitivities for each cell strain were determined. The survival curves were made with densitometry assay method for cancer cells, and with ordinary colony assay method for fibroblasts and lymphocytes. The growth rates of fibroblast were different in each strain and it was very difficult to establish the useful assay method. For cancer cells, the densitometry assay showed slightly higher survival rates than the colony assay, but there was good correlation between these two methods. On the other hand, the relationship between radiosensitivities of fibroblasts and lymphocytes and late radiation injury was not determined. The reasons were (1) number of successful cultures was short, and (2) an incidence of late radiation injury was small number. We concluded that it is important to develop the more easy and sensitive assay method, for example, measurement of some factors in the serum. In future, the developments of molecular analyzes of radiation damage can provide the some better assay methods.
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