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Measurement of extracellular pH decrease cased by hyperthermiaand drugs on tumor cells.

Research Project

Project/Area Number 04670682
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionKansai Medical University

Principal Investigator

TANAKA Yoshimasa  KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (40131445)

Co-Investigator(Kenkyū-buntansha) HARIMA Youko  KANSAI MEDICAL UNIVERSITY,FUCULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (80140276)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHeat treatment / tumor cell / intracellular pH / Fluorecent dye / 螢光色素 / 温熱耐性 / 温熱療法 / 温熱感受性 / 膜作用物質
Research Abstract

without affect of metabolic changes the authors measured intratumor pH by using 19-F magnetic resonance spectroscopy (MRS) with a fluorine compound (ZK-150471) on the basis of a curve by pH electrode.
Using the 4.7 tesla magnetic resonance apparatus, a fluorine compound that had acid-base equilibrial change and was impermeable within cell membranes was used. The fluorine compound was injected intravenously. The signal was obtained from mouse mammary cancer by creating an experimental tumor on the leg of mice, And the tumors, which were heated with and without hydralazine. The pH evaluated from chemical shift of the fluorine compound. The pH data was obtained from an electrode for reference.
The pH of nontreated tumors were 6.94. The p11 decreased to 6.722 at 20 minutes even after 20 minutes of heating.
It is possible to measure the extracellular pH by 19F-MRS with the fluorine compound noninvasively in vivo, even after heating.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Norihiko Kitada: "Evaluation of thermal damage hyperthermia on murine experimental tumor by 31P-NMR spectroscopy-Correlation between ATP and growth dalays." J of Radiat.Res.138. 65-73 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshizumi Aoki: "Measurement of intratumor pH by pH indicator used in 19F-magnetic resonance spectroscopy-Measurement of extra cellular pH decrease caused by hyperthermia conbined with hydralazin." InvestigaTive Radiology. 31. 680-689 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takuro Nishida: "Correlation between cell killing effect and cell membrane potential after heat treatment : analysis using fluorescent dye and flow cytometry" Int.J.Hyperthermia. 13. 227-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Itagaki: "Rule of intracellular calcium concentration on tumor cell death from hyperthermia" Oncology Report. 5. 139-141 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Norihiko Kitada, Kiyoshi Akagi, Yoshimasa Tanaka, Bernnardd Fritz Zieroth.: "Evaluation of thermal damage hyperthermia on murine experimental tumor by 31P-NMR spectroscopy -Correlation between ATP and growth delays" J.of Radiat.Res. 138. 65-73 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshizumi Aoki, Kiyoshi Akagi, Yoshimasa Tanaka, Jun Kawai, Masaya Takahashi: "Measurement of intratumor pH by pH indicator used in 19F-magnetic resonance spectroscopy -Measurement of extra cellular pH decrease caused by hyperthermia combined with hydralazine." Investigative Radiology. 31. 680-689 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takuro Nishida, Kiyoshi Akagi, Yoshimasa Tanaka: "Correlation between cell killing effect and cell membrane potential after heat treatment : analysis using fluorescent dye and flow cytometry" Int.J.Hyperthermia. 13. 227-234 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasushi Itagaki, Kiyoshi Akagi, Mitunobu Uda, Yoshimasa Tanaka.: "Rule of intracellular calcium concentration on tumor cell death from hyperthemia." Oncology Reports. 5. 139-141 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 永田 憲司.田中 敬正: "蛍光pH指示薬を用いた細胞内pH測定と温熱による細胞内pHへの影響" CYTOMETRY RESEARCH. 2(1). 12-17 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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