| Project/Area Number |
05453176
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Mie University |
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Principal Investigator |
NAKASHIMA Kunio Mie University Faculty of Medicine, Professor, 医学部, 教授 (40022800)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOKAWA Yoshitaka Mie Univ.Faculty of Medicine, Assistant, 医学部, 助手 (60229193)
TANAKA Minoru Mie Univ.Faculty of Medicine, Associate Professor, 医学部, 助教授 (90024736)
宇城 啓至 三重大学, 医学部, 助教授 (10151854)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Rat / Nb2 lymphoma cell / G1 cyclin / Cyclin D2 / Cyclin D3 / Gene expression / Promoter / Prolactin / プレーTリンフォーマ / Nb2 / 下細胞分化増殖 / プロククチン / Nb2細胞 / T抗原レセプターγ / サイクリンD2・D3 / シグナル伝達経路 / 遺伝子発現制御 / G1 / S移行 / T細胞分化 / T細胞抗原γ鎖 |
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| Research Abstract |
Prolactin has been demonstrated to induce the expression of the genes encoding cyclin D2 and D3 during promotion of the G1/S transition in rat Nb2 pre-T lymphoma cells. cDNA clones encoding these rat cyclins D2 and D3 were isolated and sequenced. The genes for cyclin E,cdk2, cdk5 and E2F-1 are also expressed synergistically at the same stage, suggesting the presence of a common cellular mechanism to stimulate these genes. It has also been found that, during the same G1/S transition phase, prolactin stimulates TCR-nu gene expression in 4 h and represses TCR-alpha and beta genes in the Nb2 cells. These results suggest that prolactin participates in the regulation of mammalian T-cell differentiation and proliferation.
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