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Basic study of Cultured Artificial Skin for Future Clinical Application and Its assesment for invitro Experimental Model

Research Project

Project/Area Number 05454352
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SUZUKI Shigehiko  Kyoto University Faculty of Med.Dept.of Plastic Surgery assist.Professor, 医学部, 助教授 (30187728)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsSkin Equivalent / Artificial Skin / Wound Dressing / Cultured Epithelium / Collagen / Fibroblast / Wound Healing / Experimental Model / 分化
Research Abstract

1) A new cellular artificial skin composed of an outer keratinocytic layr and an inner collagen sponge containing fibroblasts was produced and its morphology was studied ultrastructurally and immunohistochemically. The histological appearance of keratinocytic layr was similar to that of normal skin. The production of fibronectin was confirmed with immunohistochemical staining. The results of immunohistochemical staining of the keratinocytic layr in the cellular artificial skin using some kinds of anti-keratin antibodies showed almost normal differentiation.The structure of the basement membrane zone in the cellular artificial skin also resembled that of normal skin ultrastructurally.
2) The cellular artificial skin, the collagen sponge containing only fibroblasts and the acellular collagen sponge were grafted onto the skin defect produced in the back of nude mouse. The defect where the cellular artificial skin was grafted was epithelialized earliest. The defect used the collagen sponge containing fibroblasts was secondly epithelialized.
3) An acellular "bilayr artificial skin" was successfully used clinically. The newly synthesized tissue by the artificial skin after long following-up indicates more similarity to the normal dermis than the scar tissue light microscopically and electromicroscopically.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 鈴木茂彦: "新しい人工皮膚の開発" バイオインダストリー. 10. 539-545 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 鈴木茂彦: "新しい「2層性人工皮膚」の開発" 日皮協ジャーナル. 16. 68-72 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsuda K.: "Re‐freeze‐dried artificial skin." Biomaterials. 14. 1030-1035 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horiguchi Y.: "Ultra‐Structual and immunohistochemical Characterization of basal Cells in three‐dimensional culture modelsot the Skin." Archives of Dermatological Research. 286. 53-61 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maruguchi T.: "A new skin equivalenti Keratinocytes proliferated and differentiated on Collagen sponge Containing fibroblasts." Plastic and Reconstractive Surgery. 93. 537-544 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki S.: "Further application of “bilayer artificlal skin."" British Journal of Plastic Surgery. inpress.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki S.: "Advance in wound dressing and Cultured Skin Substitute" Kitasato University Edited by Kuroyanagi Y and Shioya S., 226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maruguchi T.: "Advance in wound dressing and Cultured Skin Substitute" Kitasato University Edited by Kuroyanagi Y and Shioya S., 226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsuda K.: "Re-freeze-dried artificial skin" Biomaterials. 14. 1030-1035 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horiguchi Y.: "Ultra-structual and immunohistochemical characterization of basal cells in three-dimensional culture models of the skin." Archives of Dermatological Research.286. 53-61 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maruguchi T.: "A new skin equivalent : Keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts." Plastic and Reconstructive Surgery.93. 537-544 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki S.: "Further application of "bilayr artificial skin"." British Journal of Plastic Surgery. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suzuki S.: Kitasato University.Edited by Kuroyanagi Y.and Shioya S.Advance in wound dressing and cultured skin substitute.226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maruguchi T: Kitasato University Edited by Kuroyanagi Y.and Shioya S.Advance in wound dressing and cultured skin substitute.226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Horiguchi Y.: "Ultra-structual and immunohistochemical Characterization of basal cells in three-dimensional culture models of the skin." Archives of Dermatological Research. 286. 53-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Maruguchi T.: "A new skin equivalent:Keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts." Plastic and Reconstructive Surgery. 93. 537-544 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 鈴木 茂彦: "人工皮膚の実用化" 臨床皮膚科. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] 鈴木 茂彦: "人工真皮の開発とその臨床応用" 医学のあゆみ. (印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki S.: "Further application of “bilayer artificial skin"." British Journal of Plastic Surgery.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki S: "Advance in wound dressing and cultured skin substitute" Kitasato University,Edited by Kuroyanagi Y.and Shioya,S., 78-85/226 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Maruguchi T: "Advance in wound dressing and cultured skin substitute" Kitasato University,Edited by Kuroyanagi Y.and Shioya S., 136-146/226 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki S,Matsuda K,Maruguchi T,Isshiki N: "Clinical application of a bilayer wound dressing composed of collagen sponge and silicone." Sen-i Gakkai Symposia Preprints(A).65-68 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木茂彦,松田和也: "新しい人工皮膚の開発" バイオインダストリー. 10. 539-545 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 鈴木茂彦: "新しい「2層性人工皮膚」の開発" 日皮協ジャーナル. 16. 68-72 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Matsuda K,Suzuki S,Isshiki N,Ikada Y.: "Re-freeze-dried artificial skin." Biomaterials. 14. 1030-1035 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Maruguchi T,Maruguchi Y,Suzuki S,et al.: "A new skin equivalent:Keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts." Plast.Reconstr.Surg.(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Horiguchi Y,Maruguchi T,Maruguchi Y,Suzuki S,et al.: "Ultra-structural and immunohistochemical characterization of basal cells in three-dimensional culture models of the skin." Archiv.Dermatol.Res.(in press).

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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