APPLICATION OF TISSUE CULTURE TECHNIQUE TO THE DEVELOPMENT OF ACID-SOIL TOLERANT PLANT.

• Project/Area Number: 05556010
• Research Category: Grant-in-Aid for Developmental Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: TOHOKU UNIVERSITY
• Principal Investigator: MAE Tadahiko (1994) TOHOKU UNIV.AGRIC.PROFESSOR., 農学部, 教授 (60134029); 小島 邦彦 (1993) 東北大学, 農学部, 教授 (70005669)
• Co-Investigator(Kenkyū-buntansha): YOKOTA Satoshi TOHOKU UNIV.AGRIC.ASSISTANT, 農学部, 助手 (60220555) ; 三枝 正彦 東北大学, 農学部, 教授 (10005655) ; 菅原 和夫 東北大学, 農学部, 教授 (20005672) ; 小山 博之 岐阜大学, 農学部, 助手 (90234921) ; 前 忠彦 東北大学, 農学部, 助教授 (60134029)
• Project Period (FY): 1993 – 1994
• Project Status: Completed (Fiscal Year 1994)
• Budget Amount: ¥6,600,000 (Direct Cost: ¥6,600,000); Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000); Fiscal Year 1993: ¥4,600,000 (Direct Cost: ¥4,600,000)
• Keywords: Aluminum stress / Acid soil / Tissue culture / Cultured cells / Carrot / ストレス選抜 / アルミニウム / 植物組織培養 / ストレス耐性 / ニンジン培養細胞

Research Abstract

We attempted to assess acid-soil-tolerance of carrot plants originated from calli which were selected as aluminum-tolerant (selected) cell lines. Both D1 and D2 seeds were avilable, and they were used for soil experiment and solution experiment. On typical acidic volcanic soil, root growth of selected D1 and D2 plants was more than that of non-selected D1 and D2 plants. Similar results were also obtained in the Al-stressed solution culture system. In selected plants, adsorption of Al to their root was significantly reduced. From these results, aluminum-stress selection in plant cell culture system is said to be effective method for development of new type of plants tolerant to acidic soils.

Research Products

• [Publications] Koyama H,Ojima K,Yamaya.T and Sonoda Y: "Plant-Soil Interactions at Low pH" Kluwer Academic publishers (Netherlands), 5 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yokota S and Ojima K: "Plant-Soil Interactions at Low pH" Kluwer Academic Publishers. (Netherlands), 3 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Koyama H,Ojima K,Yamaya T and Sonoda Y: Plant-soil interactions at low pH (Ed.) Date RA, "Characteristics of carrot plants regenerated from two cell lines selected as either ionic-Al tolerant cells or Al-phosphate using cells". Kluwer Academic Publishers (Netherlands), 353-357 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yokota S and Ojima K: Plant-soil interactions at low pH (Ed.), Date RA, "Physiological response of root-tip of alfalfa to low pH and aluminium stres in water culture". Kluwer Academic Publishers (Netherlands), 329-311 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Koyama,H.,Ojima,K.,Yamaya,T.,Sonoda,Y.: "Characteristics of carrot plants regenerated from two cell lines selected as either ionic-Al tolerant cells or Al-phosphate using cells." Plant-soil interactions at low pH.(Ed).Date,R.A.,Kluwer Academic Publishers,The Netherlands. (印刷中).
• [Publications] Yokota,S.,Ojima,K.: "Physiological response of root tip of alfalfa to low pH and Al stress in water culture" Plant-soil interactions at low pH.(Ed).Date,R.A.,Kluwer Academic Publishers,The Netherlands. (印刷中).