| Project/Area Number |
05558117
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
OKANO Teruo Tokyo Women's Medical College, Inst.of Biomedical Engineering, Professor, 医学部, 教授 (00130237)
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| Co-Investigator(Kenkyū-buntansha) |
SENSHU Kazuhisa Terumo Co.Inst.of Biomedical cience, 開発研究部, 研究員
SUZUKI Ken Tokyo Women's Medical College, Inst.of Biomedical Engineering, 医学部, 助手 (90216375)
NOJIRI Chisato Terumo Co.Inst.of Biomedical Science, 医科学研究所, 主任研究員
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1995: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1994: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1993: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | block copolymer / micro-domaim structure / protein absorption / nonthorombogenicty / small caliber vascular graft / 血小板 / cAMP / 細胞内遊離カルシウム濃度 / ミクロドメイン構造 / ブロックコポリマー / 抗血栓性材料 / 血小板活性化阻害 / 細胞内カルシウム |
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| Research Abstract |
Although many synthetic vascular grafts have been developed and evaluated experimentally or clinically, none of them have met long-term patency when applied as small diameter vascular substitution. We have developed a small caliber vascular graft (3 mm id.) using 2-hydroxyethylmetacrylate (HEMA) and Stirene (St) block copolymer coating. HEMA-St block copolymer formed micro-domain structure and demonstrated improved blood compatibility in both in vitro and in vivo experiments. The graft consists of three layred structure : Dacron for the outer layr, polyurethane in middle layr, and a HEMA-St block copolymer coating for the inner layr. The grafts were evaluated in dogs bilateral carotid replacement model. All grafts were patent without detachable thrombi along the graft length including anastomotic sites. Scanning electron micrographs of retrieved graft lumen showed fairly clean surfaces covered with a homogeneous protein-like layr without microthrombi or endotherial ell lining.The thickness of the surface protein layr measured by a transmission electron microscopy was about 200A which can be described as monolayr protein absorption regardless of implantation period of as much as one year. A stable monolayr absorbed protein layr formed on HEMA-St block copolymer surfaces demonstrated nonthorombogenic activities in vivo and secure long-term patency of small caliber vascular grafts with the absence of an endotherial cell lining.
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