| Project/Area Number |
05671336
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Teikyo Univ. |
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Principal Investigator |
ISHIDA Katsumi Teikyo Univ.Urology, Assos.Prof., 医学部, 講師 (90101691)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Katsumi Teikyo Univ.Urology, Assos.Prof.
|
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| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Spermatozoa / Motility / Free-Calcium / Image Analyzer / フリーカルシウムイオン / 不妊症 / フラ2-AM / 運動活性化 |
|---|
| Research Abstract |
Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.
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