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Effect and its intracellular distribution of calcium on poory motile spermot 20a.

Research Project

Project/Area Number 05671336
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionTeikyo Univ.

Principal Investigator

ISHIDA Katsumi  Teikyo Univ.Urology, Assos.Prof., 医学部, 講師 (90101691)

Co-Investigator(Kenkyū-buntansha) ISHIDA Katsumi  Teikyo Univ.Urology, Assos.Prof.
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsSpermatozoa / Motility / Free-Calcium / Image Analyzer / フリーカルシウムイオン / 不妊症 / フラ2-AM / 運動活性化
Research Abstract

Change in intracellular level and distribution of free-calcium following dilution in calcium-free or calcium-enriched medium were investigated using fluorescent image analyzer equipped with fluorescent microscope. When diluted in calcium-free media, intracellular free-calcium level elevated slightly around mitochondrial regeon in spermatozoa of golden hamster. On the contrary, the level increased significantry when diluted in calcium-enriched medium. The extracellular calcium seemed to be incorported via acrosomal regeon. In human well moved spermatozoa, the extra-cellular calcium was incorporated universally all around the head reageon, not remained only acrosomal regeon. However in poorly moved human spermatozoa, extracellular calcium was never incorporated intracellularly even though the calcium presented enough extracellularly.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] K.Ishida,H.Amemiya,T.Umeda,H.Mohri & M.Okuno: "Microflushing analysis of sperm motility" J.Reprod.Develop.39. 7-12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F Aoki,K.Ishida,M.Okuno.& K.Kohmoto: "Analysis of flogellar bending in hyperactireted hamster and mouse spermatozoa" J.Reproduct.Fert.101. 397-403 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Yoshida,K.Inaba,K.Ishida & M.Morisawa: "Calcium and ayclic AMP mediate sperm activation but Ca^<2+> alone contributes sperm chemotaxis in the asciclian,Ciona sarignyl." Develop.Growth Differ.36. 589-595 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Ishida, H.Amemiya, T.Umeda, H.Mohri and M.Okuno: "Microflushing analysis of sperm motility" J.Reprod.Develop.39. 7-12 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Aoki, K.Ishida, M.Okuno and K.Kohmoto: "Analysis of flagellar bending in hyperactivated hamster and mouse spermatozoa" J.Reprd.Fert.101. 397-403 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Yoshida, K.Inaba, K.Ishida and M.Morisawa: "Calcium and cyclic AMP mediated sperm activation, but Ca^<2+> alone contributes sperm chemotaxis in the Ascidian, Ciona savignyl" Develop.Growth Differ.36. 589-595 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Aoki,K.Ishida,M.Okano,and K.Kohimoto: "Analysis of flagellar bending in hyperactiuated hamstar and mouse spermatozoa" J.Reprod.Fert.101. 397-403 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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