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Cell Biological Study on the Response Against Mechanical Stress on Periodontal Ligament Fibroblasts Derived from Human Deciduous Teeth.

Research Project

Project/Area Number 05671726
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 矯正・小児・社会系歯学
Research InstitutionKanagawa Dental College

Principal Investigator

KIMOTO Shigenari  Kanagawa Dental College, Assistant, 歯学部, 助手 (90205013)

Co-Investigator(Kenkyū-buntansha) HOMMA Akihiko  Kanagawa Dental College, Assistant, 歯学部, 助手 (90173674)
KAWASE Toshio  Kanagawa Dental College, Assistant Professor, 歯学部, 助教授 (30084784)
SAITO Shigeru  Kanagawa Dental College, Professor, 歯学部, 教授 (80084713)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsHuman Periodontal Ligament / Fibroblast / Mechanical Stress / Cell Growth / Cell Differentiation / ヒト乳歯 / 歯根膜 / 培養細胞
Research Abstract

Periodontal ligament fibroblasts derived from human deciduous (HPLF-Y) and permanent (HPLF) teeth were established by means of explant method reported by Kawase and Saito. The effects of mechanical stress on cell growth and differentiation were studied on HPLF-Y and HPLF in culture. Those cells were loaded with intermittent mechanical strain at 5 or 10% elongation for 10 second with the resting for 10 or 50 second using the flexible bottomed culture plates and vacuum system in the presence or absence of retinoic acid (RA), 1,25 dihydroxy vitamin D_3 (D_3) or both of them. After the stretching for 24 hours, we measured alkaline phosphatase (ALPase) activity and DNA quantity of both cells. DNA synthesis of HPLF-Y and HPLF were suppressed by treatment with 0.1 mM RA for 8 days. The growth of both cells was more suppressed by RA treatment in the latter half of culture period than by D_3 or by RA and D_3 together. Alkaline phosphatase (ALPase) activity of HPLF-Y was increased by treatment w … More ith 0.1 mM RA for 8 days, however no significant change was observed on HPLF after treatment with RA at the same concentration. After the loading of intermittent stretching (5%, 3 cycle/min., 24 hr) on the day 5 in culture, cell growth of HPlF was stimulated, whereas that of HPLF-Y was suppressed. On the other hand, ALPase synthesis was stimulated on both HPLF-Y and HPLF.The mechanical stress (5%) caused significant increase of ALPase on HPLF-Y treated with D_3 or with RA and D_3 together at confluent phase. ALPase activity was increased by stretching at growth phase of HPLF treated with RA or D_3 separately or with RA and D_3 together. These data demonstrate that there are obvious differences between HPLF-Y and HPLF in terms of the response to RA or mechanical stress. The present study suggests that HPLF-Y and HPLF have different functions in the remodeling of periodontal tissue during development and that D_3 and RA are able to modify of the response to the mechanical strain on the cell growth and differentiation of HPLF and HPLF-Y. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 牧野徹夫: "ヒト乳歯および永久歯歯根膜由来線維芽細胞の細胞応答に対するL-Ascorbic Acid,L-Ascorbic Acid-2-phosphateおよび成長因子の影響について" 神奈川歯学. 28. 286-298 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 長谷川 知史: "ヒト乳歯オヨビ永久歯歯根膜由来線維芽細胞に関する細胞生化学的研究-細胞増殖と分化に対するretinoic acidおよび機械的外力の影響について-" 神奈川歯学. 28. 440-451 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 菅野 茂広: "ヒト乳歯と永久歯歯根膜由来線維芽細胞の成長因子による増殖と分化の調節" 神奈川歯学. 28. 452-462 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 吉野 練太朗: "機械的外力によるヒト歯周組織由来細胞の応答に関する研究" 神奈川歯学. 28. 166-184 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takafumi Sakamoto: "Partial Purification of Cell Attachnent and Spreading Factors Derived from the Conditioned medium of Human Periodontal Ligament Fibroblasts." Bulletin of Kanagava Dental College. 21. 25-34 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katsuhiro Nishiyama: "Effect of Mechanical Stress on Protein Synthesis in Human Periodontal Ligament Cells." Bulletin of Kanagawa Dental College. 23. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tetsuo Makino: "Effects of L-Ascorbic Acid, L-Ascorbic Acid-2-phosphate and Growth Factors on the Cellular Responses of Fibroblasts Derived from Human Deciduous and Permanent Periodontal Ligaments" KANAGAWASHIGAKU. 28-3. 286-298 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Satoshi Hasegawa: "Cytobiochemical Study on Human Periodontal Ligament Fibroblasts Derived from Deciduous and Permanent Teeth -Effects of Retinoic Acid and Mechanical Stress on Cell Growth and Differentiation-" KANAGAWASHIGAKU. 28-4. 440-451 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shigehiro Sugano: "Proliferation and Differentiation Regulated by Growth Factors on Human Periodontal Ligament Fibroblasts Derived from Deciduous and Permanent Teeth" KANAGAWASHIGAKU. 28-4. 452-462 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Rentaro Yoshino: "Cellular Response of Human Periodontium Derived Cells by Mechanical Stress" KANAGAWASHIGAKU. 28-2. 166-184 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takafumi Sakamoto: "Partial Purification of Cell Attachment and Spreading Factors Derived from the Conditioned medium of Human Periodontal Ligament Fibroblasts" Bulletin of Kanagawa Dental College. 21-1. 25-34 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Katsuhiro Nishiyama: "Effect of Mechanical Stress on Protein Synthesis in Human Periodontal Ligament Cells" Bulletin of Kanagawa Dental College. 23-1. 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 牧野 徹夫: "ヒト乳歯および永久歯歯根膜由来線維芽細胞の細胞応答に対するL-Ascorbic Acid,L-Ascorbic Acid-2-phosphateおよび成長因子の影響について" 神奈川歯学. 28. 286-298 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 長谷川 知史: "ヒト乳歯および永久歯歯根膜由来線維芽細胞に関する細胞生化学的研究-細胞増殖と分化に対するretinoic acidおよび機械的外力の影響について-" 神奈川歯学. 28. 440-451 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 菅野 茂広: "ヒト乳歯と永久歯歯根膜由来線維芽細胞の成長因子による増殖と分化の調節" 神奈川歯学. 28. 452-462 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 吉野 練太朗: "機械的外力によるヒト歯周組織由来細胞の応答に関する研究" 神奈川歯学. 28. 166-184 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takafumi Sakamoto: "Partial Purification of Cell Attachnent and Spreading Factors Derived from the Conditioned medium of Hunan Periodontal Ligament Fibroblasts." Bulletin of Kanagawa Dental College. 21. 25-34 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katsuhiro Nishiyama: "Effect of Mechanical Stress on Protein Synthesis in Human Periodontal Ligament Cells." Bulletin of Kanagawa Dental College. 23. 1-8 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 長谷川知史: "ヒト乳歯および永久歯歯根膜由来線維芽細胞に関する細胞生化学的研究-細胞増殖と分化に対するretinoic acidおよび機械的外力の影響について-" 神奈川歯学. 28. (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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