| Project/Area Number |
06454575
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | KANAGAWA DENTAL COLLEGE |
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Principal Investigator |
KINOSHITA Yukihiko KANAGAWA DENTAL COLLEGE,FACULTY OF DENTISTRY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (70084770)
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| Co-Investigator(Kenkyū-buntansha) |
KUZUHARA Takesi KANAGAWA DENTAL COLLEGE,FACULTY OF DENTISTRY,ASSISTANT, 歯学部, 助手 (40234439)
OSTUKA Toru KANAGAWA DENTAL COLLEGE,FACULTY OF DENTISTRY,ASSISTANT, 歯学部, 助手 (20168991)
KOBAYASHI Masaru KANAGAWA DENTAL COLLEGE,FACULTY OF DENTISTRY,INSTRUCTOR, 歯学部, 講師 (00162024)
IKADA Yoshito KYOTO UNIVERSITY,RESEARCH CENTER FOR BIOMEDICAL ENGINEERING,PROFESSOR, 生体医療工学センター, 教授 (00025909)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Dental Implant / Periodontal Ligament / Cell Curture / Collagen / Collagen-Immobilization / Plasma-polymerization |
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| Research Abstract |
The purpose of this study was to examine if periodontal ligament (PDL) formation can be accomplished on collagen-immobilized dental implants by the seeding of PDL cells cultured in collagen gel.
1.Culture of PDL cells in collagen gel and mitogenic resposes to growth factors
PDL cells obtained from five adult beagle dogs were cultured in collagen gel, and their mitogenic resposes to transforming growth factor -beta_1 (TGF-beta_1) and/or platelet-derived growth factor-AB (PDGF-AB) were examined. At 9 days, proliferation in cultures supplemented with TGF-beta_1 10ng/ml was 115% of control (10% FBS alone), PDGF-AB 100ng/ml was 121% of control, and the combination of both was 134% of control. The Combination also resulted in a higher level of alkaline phosphatase activity (positivecell rate : 58.8%) compared with that observed in monolayr cultures (31.3%). Therefore, culture with TGF -beta_1 10ng/ml + PDGF-AB 100ng/ml was considered to be the most suitable method for the following examination
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2.Collagen-immobilization onto dental implants
Polyethylene (PE) implants, 3.0mm in diameter and 8.0mm in hight, were prepared. Type I,III or IV aterocollagen were immobilized onto the implant surfaces by plasma polymerization method. This method resulted in a stable fixation of collagen onto the implants, and the amount of type I,III and IV immobilized were 30mug/cm^2,30mug/cm^2 and 20mug/cm^2, repectively.
3.Cell-reaction to dental implants in vitro
Type I or III collagen-immobilized PE implants were coated with 0.2ml of the PDL cell-collagen mixture (2.5*10^6 cell/ml), and were cultered for 7 days. Lightmicrosopic examination showed that PDL cells elongated their projections and formed a three-dimensional network in the gel. Electron microscopic examination revealed that cytoplasmic projections were attached to the implant surface, and newly formed collagenous fibrils were secreted through the cell membrane and deposited perpendicularly on the implant surface. These findings were not observed in non-collagen-immobilized PE implants.
4.Implantation in dogs
Implants (type I and III) coated with cell-seeded collagen gel were implanted in the lower jaws of five adult dogs. Two months after implantation, non-collagen-immobilizedimplants were encapsulated by a thick fibrous layr, fibers of which were running parallel to the implant surfaces. On the other hand, both types of collagen-immobilized implants showed a similar reaction, and they were surrounded by highly cellular and well vascularized conective tissues. However, fibers perpendicularly oriented to the implant surface as seen in the PDL were not observed. Less
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