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purification of growth stimulatory factor in rat liver plasma membrane

Research Project

Project/Area Number 06670524
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionUniversity of Tokyo

Principal Investigator

HIRATA Keiichi  University of Tokyo associated professor, 医学部(病), 助手 (50199064)

Co-Investigator(Kenkyū-buntansha) TOMIYA Tomoaki  University of Tokyo associated professor, 医学部(病), 助手 (90227637)
FUJIWARA Kenji  Saitama medical school : professor, 附属病院, 教授 (80101088)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordshepatocytes / plasma membrane / cell growth / 肝細胞増殖 / 蛋白
Research Abstract

We have reported that rat liver plasma membrane contains growth stimulatory activity, which is proven to be a proteineous comitogen of cultured rat hepatocytes.As shown by successive experiments, the factor was solubilized by organic compounds or a detergent. However, the solubilized fractions contained micellaneous proteins, when analyzed by SDS-PAGE.Fractionated proteins was not purified by column fractionation.Using mouse hybridoma cells, production of monoclonal antibody is now in progress.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Hirata K and Fujiwara K.: "Possible contribution of protein kinase C to puining for DNA synthesis induced by epidernal graoth facthr" Biochem.Biophys.Res.Comrun.171(3). 1093-1098 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirata K and Fujiwara K: "Rat liver planin manhana emharces induotion of DNA symthesis by epidermal fuctor and insalih in cultured rat hiparoiytes" Biomol.Res.12(6). 425-428 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirata, K.and Fujiwara, K.: "Possible contribution of protein kinase C activation to priming for DNA synthesis induced by cpidermal growth factor with insulin and its inhibition by plasma membrane in primary cultured rat hepatocytes." Biochem.Biophys.Res.Commun.171(3). 1093-1098 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirata, K.and Fujiwara, K.: "Rat liver plasma membrane enhances induction of DNA synthesis by cpidermal growth factor and insulin in cultured rat hepatocytes." Biomed.Res.12(6). 425-428 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tomiya T.: "Liver regeneration in fulrinant....." Hepatology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fijiwara K.,Hirata K.et al.: "The uye of Prustaglondin I_2 analog in the treatment of Massire Hepatic Necrosis Associated with Endshelial cell injnry and diffuse sinusoidal fibrin deposition." Dig.Dis.and Sci.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nagoshi S.and Fujiwara K.: "Putrescine as a comitogen of epidermal growth facta in rat lirer growth." Hepatology. 20. 725-730 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohno A.,Hirata K.,et al.: "Fat-storing cell abnormality associated with endothelial cell damage after culd ischemic storage of rat liver in UW solution." Dig.Dis.and Sci.39. 861-865 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fijiwara K.,Hirata K.,et al.: "Stimulation of liver growth by exagenous human Nepatocyte gernth factor in normal and partially hepatectomized rats." Hepatology. 18. 1443-1449 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tomiya T.,and Fujiwara K.: "Serum levels of transforming growth factor d in petients after.partinl kepatectomy as determined whth an enzyme-linked immunosorbzut assay." Hepatology. 17. 304-308 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikeda H.,and Fujiwara K.: "Evidence that impaired intracellular collagen synthesis reduces proliteration in cultured rat hepatocytes." Biochen.Biophys.Res.Commun.200. 1701-1707 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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