1995 Fiscal Year Final Research Report Summary

purification of growth stimulatory factor in rat liver plasma membrane

Research Project

Project/Area Number	06670524
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Gastroenterology
Research Institution	University of Tokyo
Principal Investigator	HIRATA Keiichi University of Tokyo associated professor, 医学部(病), 助手 (50199064)
Co-Investigator(Kenkyū-buntansha)	TOMIYA Tomoaki University of Tokyo associated professor, 医学部(病), 助手 (90227637) FUJIWARA Kenji Saitama medical school : professor, 附属病院, 教授 (80101088)
Project Period (FY)	1994 – 1995
Keywords	hepatocytes / plasma membrane / cell growth
Research Abstract	We have reported that rat liver plasma membrane contains growth stimulatory activity, which is proven to be a proteineous comitogen of cultured rat hepatocytes.As shown by successive experiments, the factor was solubilized by organic compounds or a detergent. However, the solubilized fractions contained micellaneous proteins, when analyzed by SDS-PAGE.Fractionated proteins was not purified by column fractionation.Using mouse hybridoma cells, production of monoclonal antibody is now in progress.

(4 results)