| Project/Area Number |
07405011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thermal engineering
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| Research Institution | Kanazawa University |
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Principal Investigator |
HAYASHI Yujiro Kanazawa University, Faculty of Engineering, Professor, 工学部, 教授 (30019765)
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| Co-Investigator(Kenkyū-buntansha) |
MOMOSE N. Toyama Prefectural University, Faculty of Engineering, Assistant Professor, 工学部, 助手 (80239590)
TADA Y. Kanazawa University, Faculty of Engineering, Associate Professor, 工学部, 助教授 (20179708)
TAKIMOTO A Kanazawa University, Graduate school of Natural Science and Technology, Professo, 自然科学研究科, 教授 (20019780)
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| Project Period (FY) |
1995 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥19,100,000 (Direct Cost: ¥19,100,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥14,300,000 (Direct Cost: ¥14,300,000)
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| Keywords | Cryopresevation / Biological substance / Viability / Mechanism of freezing injury / Microscale Heat Transfer / 生体細胞 / 凍結障害の機序 |
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| Research Abstract |
Freezing can slow down or stop some biological reactions for preserving the biological tissue, and thawing can bring it back to life. It is also true that freezing and thawing are lethal to the living system. During freezing, the extra- and intra-cellular ice formation, osmotic water permeation through cell membrane, deformation of cell and other behaviors occur at microscale and they bring serious injuries connecting to the life-and-death of living cells. These micro behaviors occurred on at least cell size level seem to be due to the physicochemical complexity of biological materials. Water is chemically restrained in the states of aqueous solution and colloidal solution, and those are physically confined in small spaces or compartmentalized by the cross-linked network structures.
In this research project, firstly, simplified freezing model of cell element was proposed, and the extra- and intra-cellular ice formation, osmotic water permeation through cell membrane and other microscale behavior during the freezing of biological cell were discussed as a function of temperature.
Secondly, viability of the cell was studied in relation with the micro-behaviors during freezing and thawing. In experiments, microscopic observation of wheat protoplasts was performed, and the cell viability was inspected by the fluorescence test. The osmotic conditions encountered during freeze-thaw process were also realized by osmotic manipulations. Summarizing these results, the cell damage during freezing process was estimated taking account of "solution effect injury" and "intracellular ice injury".
Thirdly, the freezing process of biological tissue was numerically simulated using heat transfer and thermodynamic model. On the basis of these results, viability of the cell during freezing of biological tissue was discussed in conjunction with the heat transfer process.
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