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Clinical and experimental study on chromosomal abnormalies detected by FISH in primary lung cancer

Research Project

Project/Area Number 07671456
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionKanazawa University

Principal Investigator

ODA Makoto (1996)  Kanazawa Univ., Depart.of Surgery (1), Assistant, 医学部・附属病院, 助手 (50224241)

清水 淳三 (1995)  金沢大学, 医学部, 講師 (50201554)

Co-Investigator(Kenkyū-buntansha) OHTA Yasuhiko  Kanazawa Univ., Depart.of Surgery (1), Assistant, 医学部・附属病院, 助手 (00272964)
WATANABE Yoh  Kanazawa Univ., Depart.of Surgery (1), Professor, 医学部, 教授 (20019897)
小田 誠  金沢大学, 医学部・附属病院, 助手 (50224241)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsFISH / chromosome 17 / p53 / DNA ploidy / PCNA / 肺癌 / 分子生物学 / FISH
Research Abstract

Objective ; We analyzed the chromosomal abnormalities in primary lung cancer cells and compared them with biological parameters of malignancy and a clinical background. Materials ; This study included 74 cases of primary non-small cell lung cancer resected at Kanazawa University Hospital between 1986-1989. Fifty-eight were male and 16 were female, and the mean age was 64.7 years. According to the TNM classification 19 were in stage I and 11,22,7,15 cases were in stage II,IIIA,IIIB,IV respectively. Methods ; We performed as follows. 1) The count of the chromosome 17 by FISH.2) The immunohistochemical staining against p53 protein. 3) The immunohistochemical staining against PCNA.4) The measurement of nuclear DNA contents by flowcytometry. Results ; 1) p53 protein expression was noted in 37 cases (50%) and observed significantly more frequently in cases of squamous cell carcinoma, cases with advanced disease and cases with DNA aneuploidy. 2) High level of PCNA was noted in 35 cases (47.3% … More ) and observed significantly more frequently in cases of squamous cell carcinoma, cases of poorly differentiated type, cases with advanced disease, cases with advanced T factor, cases with high CEA level and cases with DNA aneuploidy. 3) Abnormalities of the chromosome 17 were noticed in 22 cases (29.7%). 7 cases were monosomy (CCN1) and 15 were trisomy (CCN3). 4) The significant difference was noticed in the pathological stage, the T factor, the N factor, the histological type, the expression of p53 protein, the PCNA labeling index and the survival rate comparing CCN1 and CCN3 with CCN2.5) There was significant difference in the M factor comparing CCN1 with others, and in the pathological stage, the N factor, the histological type, the expression of p53 protein and PCNA labelling index comparing CCN3 with others. 6) There was significant correlation between FITC spots mean and the pathological stage, the N factor. 7) The prognosis was significantly better in patients with CCN2 than in those with CCN1 and CCN3 (5-year survival rate : 31.4% vs 4.9% respectively). In cases with DNA aneuploidy, the same result was obtained (5-year survival rate : 30.8% vs 5.0%). Conclusions ; Investigation and comparision of chromosomal abnormalities, oncogenes and proliferating potential clarify the degree of progression and malignancy in lung cancer. They should be taken account into when predicting the survival and deciding the treatment. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 家接 健一: "非小細胞癌の予後因子としてのp53蛋白発現,PCNA標識率およびDNA ploidyの異議" 肺癌の予後因子-研究の目的と展望-. 101-109 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akitaka Nonomura: "Clinicopathological study of primary malignant tumors of the lung" J. Surgical Oncology. 58. 5-11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 渡辺 洋宇: "肺癌の予後因子" 日本胸部臨床. 54. 535-546 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 林 義信: "I期肺癌切除例の遠隔成績と予後関連因子-生物学的悪性度を含めた総合評価から-" 日本胸部外科学会誌. 43. 672-674 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森田 克哉: "原発性肺癌切除例における肺癌細胞の腫瘍内多様性に関する研究-増殖細胞核抗原と核DNA量の二重染色による同時定量的解析-" 金沢大学十全医学会雑誌. 104. 600-610 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森田 克哉: "PCNA/DNA同時定量的解析による非小細胞肺癌の核DNA量と細胞増殖能に関する検討" 癌と化学療法. 22. 164-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 大竹 由美子: "原発性非小細胞肺癌におけるBcl-2蛋白およびp53蛋白発現の予後因子としての異議について" 金沢大学十全医学会雑誌. 106. 96-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kenichi Ietsugu, Yosinobu Hayashi, and Yoh Watanage: "p53 protein expression, PCNA,and cellular DNA content as a prognostic factor in non-small cell lung cancer" Prognostic factor in lung cancer, Nakayama shoten (in Japanese). 101-109 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akitaka Nonomura, Yuji Mizukami, Junzo Simizu, Makoto Oda, Yoh Watanabe, Takeshi Kobayashi, Ryoichi Kamimura, Tsutomu Takashima, and Masanobu Kitagawa.: "Clinicopathological study of primary malignant tumors of the lung : An analysis of 993 tumors resected at Kanazawa University Hospital between 1979-1993" J Surgical Oncology. 58. 5-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoh Wayanabe: "Prognostic factors in non-small cell lung cancer" Nippon Kyobu Rinsho (in Japanese with English abstract). 54. 535-546 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yosinobu Hayashi, Kenichi Ietsugu, katsuya Morita, Shin Nagao, Junnzo Shimizu, Shinya Murakami, Makoto Oda, Ushio Yzaki, Tetsuhiko Go, and Yoh Watanabe: "Prognosis and prognostic factors in stage I lung cancer" J Jpn.Assn.Thorac.Surg. (in Japanese with English abstract). 43. 672-674 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katsuya Morita: "Clinical and experimental studies on the intratumoral heterogeneity in lung cancer" Juzen Med.Soc. (in Japanese with English abstract). 104. 600-610 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Katsuya Morita, Yoshinobu Hayashi, Shin Nagao, Yoh Watanabe: "Relationship between cellular DNA contents and cell proloferation of non-small cell lung cancer estimated by simultaneous quntification of PCNA and DNA contents by flow cytometer" Jpn.J Cancer Chemother (in Japanese with English abstract). 22 : Supplement II. 164-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yumiko Ohtake: "Bcl-2 and p53 protein expression in non-small cell lung cancers : correlation with survival time" Juzen Med.Soc. (in Japanese with English abstract). 106. 96-104 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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