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The Effect of Nerve Growth Factor on the Genesis of Neuropathic Pain

Research Project

Project/Area Number 07671672
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionMiyazaki Medical College

Principal Investigator

UNO Takeshi  Miyazaki Medical College, Medical Department, Associate Professor, 医学部, 助教授 (70038842)

Co-Investigator(Kenkyū-buntansha) KOSAI Kazuko  Miyazaki Medical College, Medical Department, Instructor, 医学部, 助手 (00234740)
浜川 俊朗  宮崎医科大学, 医学部, 助手 (50253836)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsNerve Growth Factor / Neuropathic Pain
Research Abstract

We observed the effect of nerve growth factor (NGF) on a noxious behavior in rat neuropathic pain model. [Methods] Male Sprague-Dawley rats weighing 200-250g at the biginning of the experiment were used. The rats were anesthetized with sodium pentobarbital (50mg/kg, i.p.) , and the right sciatic nerve was ligated with 4-0 chromic gut suture under microscopic observation. At the level of the mid-thigh, four loose ligatures were done just proximal to its trifurcation with 1-2mm distance between each ligature. Then, osmotic minipump connected to sillicone tube was implanted subcutaneously in the back for the continuous infusion of NGF (human recombinant NGF,1mug/day) or vehicle (sodium phosphate buffer, pH=7.0, containing 10%BSA) for 2 weeks. Thermal hyperalgesia was assessed before sciatic nerve ligation and 1,3,5,7 postoperative day. Thermal noxious threshold was determined to measure withdrawal latency against radiant heat which was applied to a plantar surface of each hind paw by using of plantar test (UGO BASILE) The magnitude of hyperalgesia was estimated by difference score (DS) which was obtained by subracting the latency of the opposite side from that of the ligated. Therefore, negative scores indicate thermal hypralgesia. [Results] DS in control group increased negatively from 3 to 7 postoperative day, whereas DS in NGF group did not increase negatively compared to a control. [Conclusion] We concluded that NGF could be useful for the treatment of neuropathic pain in clinical situation.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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