| Project/Area Number |
07671979
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
TAKEDA Masako Dentistry, Anatomy, Health Sciences University of Hokkaido Professor, 歯学部, 教授 (40001953)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | taste bud / denervation / apoptosis / colchicine / circumvallate papilla / epithelial cell / cell death / マウス / 舌咽神経 |
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| Research Abstract |
Apoptotic cells in the taste buds of mouse circumvallate papillae after the sectioning of bilateral glossopharyngeal nerves and the treatment with colchicine (inducer of microtubules depolimerization) were examined by the method of DNA nick-end labeling (TUNEL), together with standard electron microscopy. The taste buds decreased in number and size 3-11 days after denervation and disappeared at 11 days. The TUNEL method revealed only a few positively stained nucliei in normal taste buds but, in those of mice 1-5 days after denervation, the number of positive nuclei had increased to 3-5 times that of taste buds from normal mice. Electron-microscopic obsevation after denervation demonstrated taste bud cells containing codensed and fragmentary nuclei in a cytoplasm with increased density. The colchicine treatment after 15 hours and 1 day showed many TUNEL-positive nuclei and the condensed and fragmentary nuclei in the taste bud cells.
The results show that taste bud cells under normal conditions die by apoptosis at the end of their life span, and that gustatory nerve sectioning and colchicine treatment cause apoptosis of taste bud cells.
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