| Project/Area Number |
07680668
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
NAKAMURA Toshiya School of Allied Medical Sciences, Associate Professor, 医学部, 助教授 (00155847)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MUNAKATA Hidekazu School of Medicine, Research Associate, 医学部, 助手 (80271807)
TAKAGAKI Keiichi School of Medicine, Associate Professor, 医学部, 助教授 (70163160)
|
|---|
| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
|
|---|
| Keywords | Hyaluronic acid / Inhibitor / Extracellular matrix / Human skin fibroblasts / 4-Methylumbelliferone / Decorin |
|---|
| Research Abstract |
Cultivation of human skin fibroblasts in the presence of 0.5 mM 4-methylumbelliferone repressed hyaluronan production of the cell and reduced its chain length, resulting establishment of hyaluronan-deficient extracellular matrix. Cell-free experiments suggested that 4-methylumbelliferone may repress the expression of hyaluronan synthase on the cell surface. Electron-microscopic analysis of the hyaluronan-deficient extracellular matrix showed decrease of extracellular matrix component morphologically. Analysis of hyaluronan-binding proteins in medium showed the decrease of the amount of hyaluronan binding proteins, especially 83kDa protein, in the medium of the cells cultured in the presence of 4-methylumballiferone. Synthesis of proteoglycans of the cells were not affected by 4-methylumbelliferone signifcantly except for that of decorin. The synthesis of decorin was stimulated by 4-methylumbelliferone and, moreover, the number of glycosaminoglycan chains of decorin was increased from 1 to 2 by 4-methylumbelliferone.
|
