| Project/Area Number |
07807138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SARUHASHI Yasuo Shiga University of Medical Science, Department of Orthopaedic surgery, instrucor, 医学部, 助手 (40273403)
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| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | stretch activated channel / single channel / patch clamp / osteoblast / カリウムチャネル / 伸展活性化チャネル / 線維芽細胞 |
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| Research Abstract |
A clonal cell line, HuO9, was recently established from a human osteosarcoma (Kawai1A.Olin Orthop Res , 1990,259,256-267). HuO9 cells are useful for studies on characteristics of human osteoblasts because HuO9 cells retain a high activity of alkaline phosphatase and secreted osteocalcin. We identified the potassium channel in HuO9 cells cultured for 2-14 days by patch-clamp, single-channel-recording techniques. So far the following properties were observed. Open probability of K+ channel (122 pS) depended upon membrane potentials and Ca concentration of bath solutions. Depolarization and high Ca concentration activated the K+ channel. Applied negative pressure to pipette also increased the open probability of the channel. jpn. J.Physiol. , VoI.48.Suppl. 1998
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