• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic studies on production and application of transgenic animals in ruminants

Research Project

Project/Area Number 08406018
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionThe University of Tokyo

Principal Investigator

TOJO Hideaki (1997-1998)  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20041668)

舘 鄰 (1996)  東京大学, 大学院・農学生命科学研究科, 教授 (30011711)

Co-Investigator(Kenkyū-buntansha) YAMANOUCHI Keitaro  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assi, 大学院・農学生命科学研究科, 助手 (70272440)
SAKAI Senkichi  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (80114487)
SAWASAKI Tooru  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (00012047)
TKAHASHI Michio  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Prof, 大学院・農学生命科学研究科, 教授 (30011943)
TACHI Chikashi  Azabu University, School of Veterinary Sciences, Professor, 獣医学部, 教授 (30011711)
東條 英昭  東京大学, 大学院・農学生命科学研究科, 助教授 (20041668)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥34,300,000 (Direct Cost: ¥34,300,000)
Fiscal Year 1998: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1997: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1996: ¥21,000,000 (Direct Cost: ¥21,000,000)
Keywordstransgenic / homeobox / c-kit / mouse / caprine hircus / EGFP / 形質転換家畜 / 遺伝子導入 / α-ガラクトース / 制限酵素 / 蛍光蛋白 / 胚 / トランスジーン / シバヤギ / ホメオボックス / c-kit / SCF
Research Abstract

1. The complete open reading tram of the c-kit cDNA was clone from the Shiba goat (Capra hircus var Shiba) with the dominant black-eyed white phenotype. The analysis of the deduced amino acid sequence revealed the presence of a single amino acid insertion (alanin) in the kinase insert region. 2. The expressions of homeobox genes in murine and caprine placentae were surveyed. In the murine placentae, all the Hox 9 paralogues except for Hox c9 were expressed. In the caprine placentae, the HOX 9 paralogues except for HOX d9 were detected. 3. Attempts were made to investigate whether co-injection of foreign DNA constructs with bacterial restriction endonuciease into the pronucleus of mouse zygotes will be capable of elevating integration frequency of foreign DNA into the host genome. The results showed that co-injection of the genes with restriction enzyme appeared the slight elevation of successful integration in the embryos and the pups. 4. One way to improve the efficiency of transgenic animal production would be to identify the transgenic embryos before transfer into foster mothers. Green fluorescent protein (GFP) can be a suitable marker for the selection of transgenic embryos. CMV beta-actin/EGFP gene was microinjected into the pronucleus of mouse zygotes. The DNA injected embryos were cultured in vitro and observed for fluorescence. The fluorescent embryos were transferred into the uteri of recipient mice. Pups born were analyzed for transgenic. A part of the embryos were analyzed for the transgene by the Dpn I-Bal31 digestion method. The results showed that approximately 77% of the embryos with uniform fluorescence were transgenic. The present study demonstrated that the method using EGEP as a marker be very reliable for selecting the transgenic embryos.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Ikeda A: "Different female reproductive phenotypes determined by human growth hormone (hGH) levels in hGH-transgenic rats" Biol.Reprod.,. 56. 847-851 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo B.B: "Efficient selection of preimplantaion transgenic embryos by the improved procedures of the Dpn I-Bal 31 digestion and polymerase chain reaction" Reprod.Fetril.Dev.,. 9. 263-269 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S: "Cell-cell contact down-regulates expression of membrane type metalloproteinase-I (MT-MMP) in a mouse mammary gland epitherial cell line" Zool.Sci.,. 14. 95-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sasaki F: "Growth hormone releasing hormone (GHRH)^GH-somatic growth and luteinizing hormone (LH) RH-LH-ovarian axes in adult female transgenic mice expressing human GH gene." J.Neuroendocri.,. 9. 615-626 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyooka Y: "Wilms' tumor suppressor gene (WT1) as a target gene of SRY function in a mouse ES cell line transfected with SRY." Int.J.Dev.Biol.42. 1143-1151 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano K: "Skeletal muscle of transgenic mice expressing human sonoN,a homologue of c-ski." J.Reprod.Develop.,. 44. 253-260 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 東條英昭: "動物をつくる遺伝子工学" 講談社, 227 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S,et al.: "Molecular cloning of c-kit encoding the c-kit receptor of Shiba goats and a novel alanine insertion specific to goats and sheep in the kinase insert region." Biochem.Biophys.Acta.1352. 151-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S,et al: "Cell-cell contact down-regulates expression of membrane type metalloproteinase-1 (MT-MMP) in a mouse mammary glad epitherial cell line." Zool.Sci.14. 95-99 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seo B.B,et al: "Efficient selection of preimplantation transgenic embryos by the improved procedures of the Dpm I-Bal 3I digestion and polymerase chain reaction" Reprod.Fetril.Dev.9. 263-269 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Inoue K,et al.: "Quantitative analysisi of striped coat-color patterns in large white/Duroc chimeric pigs with special reference to the genetic control mechanisms of the dominant black eyed white phenotype." Pigment Cell Res.9. 289-297 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda A,et al: "Different female reproductive phenotypes determined by human growth hormone (hGH) levels in hGH-transgenic rats" Biol.Reprod.56. 847-851 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sasaki F,et al: "Growth hormone releasing hormone (GHRH)^GH-somatic growth and luteinizing hormone (LH) RH-LH-ovarian axes in adult female transgenic mice express-ing human GH gene." J.Neuroendocri.9. 615-626 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamanouchi K.: "Equine inhibin/actibin subunit is expressed in the endometrial gland, but not in the trophoblast, during pregnancy." J.Reprod.Devlop.47. 363-369 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamanouchi K,et al: "Expression and cellular localization of inhibin alpha-subunit mRNA in equine fetal gonads." J.Vet.Med.Sci.59. 569-573 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yamanouchi K,et al: "Studies on expression of the c-ski gene in equine (Thouroughbred) tissues." J.Equine Sci.8. 13-19 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kano K,Tojo H,et al.: "Skeletal muscle of transgenic mice expressing human snoN,a homologue of c-ski." J.Reprod.Develop.44. 253-260 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyooka Y,et al.: "Wilms'tumor suppressor gene (WT1) as a target gene of SRY function in a mouse ES cell line transfected with SRY." Int.J.Dev.Biol.42. 1143-1151 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikeda A: "Different female reproductive phenotypes determined by human growth hormone (hGH) levels in hGH-transgenic rats." Biology of Reproduction.56. 847-851 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sasaki F: "Growth hormone releasing hormone-GH-somatic growth and luteininzing hormone RH-LH-ovarian axes in adult female transgenic mice" Journal of Neuroendocrinology. 9. 615-626 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka S: "Molecular cloning of c-kit encoding the c-kit receptor of Shida goats and a novel alanine insertion specific to goats and sheep inthe kinase." Biochemica Biophysica Acta.1352. 151-155 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Seo B.B: "Efficient selection of preimplantaion transgenic embryos by the improved of the Dpn l-Bal 31 digestion and polymerase chain reaction." Reproduction, Fertility and Development. 9. 263-269 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kano K: "Skeletal muscle of transgenic mice expressing human snoN, a homologue of c-ski." Jouranl of Reproduction and Development. 44. 253-260 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toyookoa Y: "Wilm's tumor suppressor gene (WT1) as a target gene of SRY function in a mouse ES cell line transfected with SRY." International Journal of Development and Biology. 42. 1143-1151 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Suenaga A: "Quantitative analysis of the spreading of the mouse trophoblast in vitro : a model for early invasion." Placenta. 17. 583-590 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirota O.: "Effects of transfected human and mouse SRY/Sry genes upon the expression of hypothtical SRY・caseade genes in cultured mouse sertoli cell" Journal of Reproduction and Development. 42. 255-265 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hase W: "Transgenic mice overexpressing forein whey acidie WAP genes driven by methallothionein promoter." Journal of Reproduction and Development. 42. 235-271 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagasawa H: "Changes of plasma level of human growth hormone with age in relation to mammary tumour appearance in wheyacidic protein/human" in vivo. 10. 503-506 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seo B.B: "Efficient selection of preimplantation transgenic embryos by the improved procedures of the Dpn1-Bal 31 digestion and polymerase chain" Reproduction.Fertility and Development. 9. 263-269 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sasaki F: "Growth hormone releasing hormone (GHRH) GH-somatic growth and luteininzing hormone (LH) RH-LH-ovarian axes in adult female transgenic" Journal of Neuroendocrinology. 9. 615-626 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 東條英昭: "動物をつくる遺伝子工学" 講談社, 227 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suenaga A: "Quantitative analysis of the spreading of the mouse trophoblast in vitro:a model for early invasion." Placenta. 17. 583-590 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirota O.: "Effects of transfected human and mouse SRY/Sry genes upon the expression of hypothtical SRY-cascade genes in cultured mouse sertoli cell." Journal of Reproduction and Development. 42. 225-265 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hase W.: "Transgenic mice overexpressing forein whey acidic WAP genes driven by methallothionein promoter." Journal of Reproduction and Development. 42. 265-271 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanaka S.: "Cell-cell contact down-regulates expression of membrane type metallo-proteinase(MT-MMP)in a mouse mammary gland epithrial cell line." Zoological Science. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Seo B.B,: "Efficient selection of preimplantation transgenic embryos by the improved procedures of the DpnI-Bal 31 digestion and polymerase chain" Reproduction.Fertility and Development. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Sasaki F: "Growth hormone releasing hormone(GHRH)GH-somatic growth and luteininzing hormone(LH)RH-LH-ovarian axes in adult female transgenic" Journal of Neuroendocrinology. (印刷中).

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi