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Development of novel diagnosis method for minutecancer by infrared fluorescence endoscopy using fluorescence-emitting antibody by infrared ray excitation.

Research Project

Project/Area Number 08457168
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionThe University of Tokushima

Principal Investigator

ITO Susumu  The University of Tokushima, Institute for Enzyme Research Professor, 医学部, 教授 (70093838)

Co-Investigator(Kenkyū-buntansha) KIDO Hiroshi  The University of Tokushima, Institute for Enzyme Research Professor, 分子酵素学研究センター, 教授 (50144978)
II Kunio  Bis-President Japan Neulogy Institute, 副所長(研究職) (50035507)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥4,800,000 (Direct Cost: ¥4,800,000)
KeywordsICG Delivative / Endoscopic Diagnosis / Fluorescence Marker / Infrared Fluorescence / Infrared Fluorescenct Endoscopy / Vital Immunohistochemical staining
Research Abstract

ICG-sulfo-OSu (ICG N-hydroxy sulfo succinimide ester), as the labeling material that is fluorescent on excitation with infrared ray(IR) (Bioorg Med Chem Letters 1995 ; 5 : 2689-94, Bioorganic &Medicinal Chemistry 6(11) : 2179-2184, 1998) and the imaging system for IR fluorecent microscope(Dig Endosc 1997 ; 278-82) were developed in this experiment. The intensity of fluorescence of ICG-sulfo-OSu is too low for detection of microlesions by IR imaging system. Therefore, in the present study, we developed an imaging system using a highly sensitive charge-coupled-device (ICCD) (Digestive Endoscopy 11, 1999 (in press)) and the reinforcement agents (Bioorganic &Medicinal Chemistry 6(5) : 613-618, 1998) that can be used to image this labeling substance at the tissue level. Therefore, provide the fact that some antibodies which are specific to cancer cells can detect cancer cells by IR fluorescence also by using such antibodies bound with ICG.(J Gastroenterol 1998 ; 33 : 467-71. J.Medical Investigation 46, 1999 (in press), Digestive Endoscopy 11, 1999 (in press))

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Ito S: "Development of an imaging system using fluorescent labeling substances excited by infrared rays." Digestive Endoscopy. Vol.9 No.4. 278-282 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito S: "Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining." Bioorganic & Medicinal Chemistry. Vol.6. 613-618 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Muguruma N: "Antibodies labeled with fluorescence-agent excitable by infrared rays." J Gastroenterol. Vol.33 No.4. 467-471 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirata T: "Synthesis and reactivities of 3-indocyanine-green-acyl-1,3-thiazolidine-2-thione (ICG-ATT) as a new near-infrared fluorescent-labeling reagent." Bioorganic & Medicinal Chemistry. Vol.6. 2179-2184 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taoka S: "Reflected Illumination-Type Imaging System for the DevelopmenInfrared Fluorescence Endoscopy." Digestive Endoscopy. Vol.11 in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayashi S: "The Basic Study on Vital Immunohistochemical Staining for A Novel Method of Diagnosis with Video Endoscopy (Examination of immunohistochemical staining to non-fixed fresh tissue)." J.Medical Investigation. Vol.46 in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito S, Muguruma N, Hayashi S, Taoka S, Tsutsui A, Fukuda T, Okahisa T, Ohkita Y, Matsunaga H, Shimizu I, Nakamura k, Imaizumi K, Takesako K, Shibamura S: "Development of an imaging system using fluorescent labeling substances excited by infrared rays." Digestive Endoscopy. 9 (4). 278-282 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ito S, Muguruma N, Hayashi S, Taoka S, Bando T, Inayama K, Sogabe M, Okahisa T, Okamura S, Shibata H, Irimura T, Takesako K, Shibamura S: "Development of agents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining." Bioorganic & Medicinal Chemistry. 6. 613-618 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Muguruma N, Ito S, Hayashi S, Taoka S, Kakehashi H, Ii K, Shibamura S, Takesako K: "Antibodies labeled with fluorescence-agent excitable by infrared rays." J Gastroenterol. 33 (4). 467-471 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirata T, Kogiso H, Morimoto K, Miyamoto S, Taue H, Sano S, Muguruma N,Ito S, Nagao Y: "Synthesis and reactivities of 3-indocyanine-green-acyl-1, 3-thiazolidine-2-thione (ICG-ATT) as a new near-infrared fluorescent-labeling reagent." Bioorganic & Medicinal Chemistry. 6. 2179-2184 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Taoka S, Ito S, Muguruma N, Hayashi S, Kusaka Y, Ii K, Nakamura K, Imaizumi K, Takesako K, Shibamura S: "Reflected Illumination-Type lmaging System for the the DevelopmenInfrared Fluorescence Endoscopy." Digestive Endoscopy. 11 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hayashi S, Muguruma N, Bando T, Taoka S, Ito S, Ii K: "The Basic Study on Vital Immunohistochemical Staining for A Novel Method of Diagnosis with Video Endoscopy (Examination of immunohistochemical staining to non-fixed fresh tissue)." J.Medical lnvestigation. 46 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Susumu Ito: "Development of aqents for reinforcement of fluorescence on near-infrared ray excitation for immunohistological staining" Bioorganic & Medicinal Chemistry. Vol.6,No.6. 613-618 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N Muguruma: "Antibodies labeled with fluorescent-agent excitable by infrared rays" Journal of Gastroenterology. Vol.33,No.4. 467-471 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] N.Muguruma: "A new diagnostic method using an infrared endoscope for digestive tract micro-lesions" Gastroenterology. Vol.112 No.4. A620 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Ito: "Development of an imaging system using fliorescent labelling substances excited by infrared rays" Digestive Endoscopy. Vol.9 No.4. 278-282 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Ito: "Development of new diagnostic method for digestive tract micro-lesions using fluorecent substances exciteble by infrared rays" Endoscopy. Vol.29 No.7. E17 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Muguruma: "Development of labeled antibodies using fluorescence emitting substance upon excitation with infrared rays" Journal of Gastroenterology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Susumu Ito: "Possibility of an Infrared ray fluorescent videoendos copy for diagnosis of minute cancer by vital immunostaining" 10th Asian-Pasific Congress of Gastroenterology(APCDE)/7th Asian-Pasific Congress of Digestive Endoscopy.(APCDE). (Abstracts). 499 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Naoki Muguruma: "Development of vital immunostaining by flourescen ce-labelled antibody under near infrared ray excitation" 10th Asian-Pasific Congress of Gastroenterology(APCDE)/7th Asian-Pasific Congress of Digestive Endoscopy.(APCDE). (Abstracts). 499 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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