| Project/Area Number |
08458169
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioorganic chemistry
|
|---|
| Research Institution | Tohoku Univ. |
|---|
Principal Investigator |
NISHINO Tokuzo Tohoku Univ., Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (90005827)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大沼 信一 東北大学, 工学部, 助手 (30221831)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥8,700,000 (Direct Cost: ¥8,700,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥7,500,000 (Direct Cost: ¥7,500,000)
|
|---|
| Keywords | Prenyltransferase / farnesyl diphosphate / Escherichia coli / mutagenesis / geranylgeranyl diphosphate / ファルネシルニリン酸 |
|---|
| Research Abstract |
1.We detected two novel prenyltransferase activities in a farnesyl transferase null mutant of Escherichia coli. Although the amounts of these enzymes are slight, they are thought to substitute for the detected enzyme. They can be purified by adding a detergent into buffer solution, like membrane proteins. one of them was characterized as octaprenyl diphosphate synthase, and the other was shown to synthesize farnesyl diphosphate and longer prenyl diphosphates
2.We tried to elucidate the mechanism by which prenyltransferases decided chain length of their products, aiming at production of prenyl diphosphates in Escherichia coli. Using random mutagenesis technique, we succeeded to change chain length of the products.of prenyltransferases derived from a thermophilic eubacteria and a high-degree thermophilic archaea, and revealed that an amino acid residue which locates on the fifth position before the first of two aspartate-rich motifs, which are highly conserved between prenyltransferases, is important for the mechanism of product detemination. They were the first reports on the artificial control of the product's chain length of prenyltransferases. Besides, we compared surrounding regions of the important amino acid residues of various prenyltransferases and mutagenized them according to the result of comparison. Therefore we found that the mechanisms of product determination of prenyltransferases can be differentiated phylogenctically.
|
